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Comparative mapping of quantitative trait loci associated with waterlogging tolerance in barley (Hordeum vulgare L.).

Li H, Vaillancourt R, Mendham N, Zhou M - BMC Genomics (2008)

Bottom Line: Twenty QTLs for waterlogging tolerance related traits were found in the two barley double haploid (DH) populations.Improving waterlogging tolerance in barley is still at an early stage compared with other traits.QTLs identified in this study have made it possible to use marker assisted selection (MAS) in combination with traditional field selection to significantly enhance barley breeding for waterlogging tolerance.

View Article: PubMed Central - HTML - PubMed

Affiliation: Tasmanian Institute of Agricultural Research and School of Agricultural Science, University of Tasmania, P,O, Box 46, Kings Meadows, TAS 7249, Australia. haobingl@utas.edu.au

ABSTRACT

Background: Resistance to soil waterlogging stress is an important plant breeding objective in high rainfall or poorly drained areas across many countries in the world. The present study was conducted to identify quantitative trait loci (QTLs) associated with waterlogging tolerance (e.g. leaf chlorosis, plant survival and biomass reduction) in barley and compare the QTLs identified across two seasons and in two different populations using a composite map constructed with SSRs, RFLP and Diversity Array Technology (DArT) markers.

Results: Twenty QTLs for waterlogging tolerance related traits were found in the two barley double haploid (DH) populations. Several of these QTLs were validated through replication of experiments across seasons or by co-location across populations. Some of these QTLs affected multiple waterlogging tolerance related traits, for example, QTL Qwt4-1 contributed not only to reducing barley leaf chlorosis, but also increasing plant biomass under waterlogging stress, whereas other QTLs controlled both leaf chlorosis and plant survival.

Conclusion: Improving waterlogging tolerance in barley is still at an early stage compared with other traits. QTLs identified in this study have made it possible to use marker assisted selection (MAS) in combination with traditional field selection to significantly enhance barley breeding for waterlogging tolerance. There may be some degree of homoeologous relationship between QTLs controlling barley waterlogging tolerance and that in other crops as discussed in this study.

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Related in: MedlinePlus

Comparison of quantitative trait loci (QTLs) identified for waterlogging tolerance in two different barley doubled haploid populations: tf = Franklin/TX9425; yf = Franklin/Yerong. Markers flanking the one LOD support interval of each QTL identified in the individual population were re-located on a barley composite map [35] so that their relative position could be compared. Centromeres are indicated as in [35]. A general name (such as Qwt1-1) was given to each chromosome region associated with waterlogging tolerance, the first number was the chromosome number and the second number was the serial number of regions identified on that chromosome.
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Figure 3: Comparison of quantitative trait loci (QTLs) identified for waterlogging tolerance in two different barley doubled haploid populations: tf = Franklin/TX9425; yf = Franklin/Yerong. Markers flanking the one LOD support interval of each QTL identified in the individual population were re-located on a barley composite map [35] so that their relative position could be compared. Centromeres are indicated as in [35]. A general name (such as Qwt1-1) was given to each chromosome region associated with waterlogging tolerance, the first number was the chromosome number and the second number was the serial number of regions identified on that chromosome.

Mentions: In order to compare the QTLs identified in different populations, the markers flanking the one LOD support intervals for each QTL were relocated on the consensus map [35] including the two populations used in this study. Comparison of the identified QTLs between the two populations (Table 5; Figure 3) showed that many of the QTLs identified in Franklin/TX9425 mapped to similar chromosomal regions compared to those identified in Franklin/Yerong (such as QTLs identified on chromosome 3H and 7H), or mapped very close to one another with almost touching or overlapping two LOD support intervals (such as QTLs identified on chromosome 1 H, 2H and 4H) (Figure 3).


Comparative mapping of quantitative trait loci associated with waterlogging tolerance in barley (Hordeum vulgare L.).

Li H, Vaillancourt R, Mendham N, Zhou M - BMC Genomics (2008)

Comparison of quantitative trait loci (QTLs) identified for waterlogging tolerance in two different barley doubled haploid populations: tf = Franklin/TX9425; yf = Franklin/Yerong. Markers flanking the one LOD support interval of each QTL identified in the individual population were re-located on a barley composite map [35] so that their relative position could be compared. Centromeres are indicated as in [35]. A general name (such as Qwt1-1) was given to each chromosome region associated with waterlogging tolerance, the first number was the chromosome number and the second number was the serial number of regions identified on that chromosome.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2533678&req=5

Figure 3: Comparison of quantitative trait loci (QTLs) identified for waterlogging tolerance in two different barley doubled haploid populations: tf = Franklin/TX9425; yf = Franklin/Yerong. Markers flanking the one LOD support interval of each QTL identified in the individual population were re-located on a barley composite map [35] so that their relative position could be compared. Centromeres are indicated as in [35]. A general name (such as Qwt1-1) was given to each chromosome region associated with waterlogging tolerance, the first number was the chromosome number and the second number was the serial number of regions identified on that chromosome.
Mentions: In order to compare the QTLs identified in different populations, the markers flanking the one LOD support intervals for each QTL were relocated on the consensus map [35] including the two populations used in this study. Comparison of the identified QTLs between the two populations (Table 5; Figure 3) showed that many of the QTLs identified in Franklin/TX9425 mapped to similar chromosomal regions compared to those identified in Franklin/Yerong (such as QTLs identified on chromosome 3H and 7H), or mapped very close to one another with almost touching or overlapping two LOD support intervals (such as QTLs identified on chromosome 1 H, 2H and 4H) (Figure 3).

Bottom Line: Twenty QTLs for waterlogging tolerance related traits were found in the two barley double haploid (DH) populations.Improving waterlogging tolerance in barley is still at an early stage compared with other traits.QTLs identified in this study have made it possible to use marker assisted selection (MAS) in combination with traditional field selection to significantly enhance barley breeding for waterlogging tolerance.

View Article: PubMed Central - HTML - PubMed

Affiliation: Tasmanian Institute of Agricultural Research and School of Agricultural Science, University of Tasmania, P,O, Box 46, Kings Meadows, TAS 7249, Australia. haobingl@utas.edu.au

ABSTRACT

Background: Resistance to soil waterlogging stress is an important plant breeding objective in high rainfall or poorly drained areas across many countries in the world. The present study was conducted to identify quantitative trait loci (QTLs) associated with waterlogging tolerance (e.g. leaf chlorosis, plant survival and biomass reduction) in barley and compare the QTLs identified across two seasons and in two different populations using a composite map constructed with SSRs, RFLP and Diversity Array Technology (DArT) markers.

Results: Twenty QTLs for waterlogging tolerance related traits were found in the two barley double haploid (DH) populations. Several of these QTLs were validated through replication of experiments across seasons or by co-location across populations. Some of these QTLs affected multiple waterlogging tolerance related traits, for example, QTL Qwt4-1 contributed not only to reducing barley leaf chlorosis, but also increasing plant biomass under waterlogging stress, whereas other QTLs controlled both leaf chlorosis and plant survival.

Conclusion: Improving waterlogging tolerance in barley is still at an early stage compared with other traits. QTLs identified in this study have made it possible to use marker assisted selection (MAS) in combination with traditional field selection to significantly enhance barley breeding for waterlogging tolerance. There may be some degree of homoeologous relationship between QTLs controlling barley waterlogging tolerance and that in other crops as discussed in this study.

Show MeSH
Related in: MedlinePlus