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Unusually stable abnormal karyotype in a highly aggressive melanoma negative for telomerase activity.

Gagos S, Papaioannou G, Chiourea M, Merk-Loretti S, Jefford CE, Mikou P, Irminger-Finger I, Liossi A, Blouin JL, Dahoun S - Mol Cytogenet (2008)

Bottom Line: Interestingly this tumor had no detectable telomerase activity as indicated by the Telomere Repeat Amplification Protocol.Telomeric Flow-FISH and quantitative telomeric FISH on mitotic preparations showed that malignant cells had relatively short telomeres.Microsatellite instability was ruled out by the allelic pattern of two major mononucleotide repeats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Genetics, Biomedical Research Foundation of the Academy of Athens, Greece (BRFAA). sgagos@bioacademy.gr.

ABSTRACT
Malignant melanomas are characterized by increased karyotypic complexity, extended aneuploidy and heteroploidy. We report a melanoma metastasis to the peritoneal cavity with an exceptionally stable, abnormal pseudodiploid karyotype as verified by G-Banding, subtelomeric, centromeric and quantitative Fluorescence in Situ Hybridization (FISH). Interestingly this tumor had no detectable telomerase activity as indicated by the Telomere Repeat Amplification Protocol. Telomeric Flow-FISH and quantitative telomeric FISH on mitotic preparations showed that malignant cells had relatively short telomeres. Microsatellite instability was ruled out by the allelic pattern of two major mononucleotide repeats. Our data suggest that a combination of melanoma specific genomic imbalances were sufficient and enough for this fatal tumor progression, that was not accompanied by genomic instability, telomerase activity, or the engagement of the alternative recombinatorial telomere lengthening pathway.

No MeSH data available.


Related in: MedlinePlus

The cytologic examination of the ascitic fluid showed malignant cells with high mitotic index (Giemsa × 400) (A). Immunocytochemistry against the melanocyte specific antibody S-100 confirmed the presence of malignant melanocytes (Hematoxylin and DAB × 400).
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Figure 1: The cytologic examination of the ascitic fluid showed malignant cells with high mitotic index (Giemsa × 400) (A). Immunocytochemistry against the melanocyte specific antibody S-100 confirmed the presence of malignant melanocytes (Hematoxylin and DAB × 400).

Mentions: Peritoneal fluid samples were obtained by two subsequent paracenteses (within a 12-day interval) of a 38-year-old woman, presented at the Department of Gynecology, Laikon Hospital, with ascites and solid structures at her ovaries as revealed by CT-scan. Two years ago the patient had a less than 1.5 cm large, cutaneous nevus excised from the anterior surface of her left hip. The primary tumor was characterized as a nodular melanoma, Clark's level 3, Breslow's depth 2.0 mm. One out of 14 inguinal nodes, excised in a subsequent operation, was found to be invaded. She received 6 cycles of chemotherapy (cis-platin-dacarbazine) and remained disease-free for 15 months. The cytologic examination of the ascitic aspiration confirmed the presence of malignant cells positive for the melanocyte specific antibody S-100 (Figure. 1). The patient refused to be operated, gave her written consent for further research on the specimens obtained, and expired 40 days after presentation.


Unusually stable abnormal karyotype in a highly aggressive melanoma negative for telomerase activity.

Gagos S, Papaioannou G, Chiourea M, Merk-Loretti S, Jefford CE, Mikou P, Irminger-Finger I, Liossi A, Blouin JL, Dahoun S - Mol Cytogenet (2008)

The cytologic examination of the ascitic fluid showed malignant cells with high mitotic index (Giemsa × 400) (A). Immunocytochemistry against the melanocyte specific antibody S-100 confirmed the presence of malignant melanocytes (Hematoxylin and DAB × 400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2533344&req=5

Figure 1: The cytologic examination of the ascitic fluid showed malignant cells with high mitotic index (Giemsa × 400) (A). Immunocytochemistry against the melanocyte specific antibody S-100 confirmed the presence of malignant melanocytes (Hematoxylin and DAB × 400).
Mentions: Peritoneal fluid samples were obtained by two subsequent paracenteses (within a 12-day interval) of a 38-year-old woman, presented at the Department of Gynecology, Laikon Hospital, with ascites and solid structures at her ovaries as revealed by CT-scan. Two years ago the patient had a less than 1.5 cm large, cutaneous nevus excised from the anterior surface of her left hip. The primary tumor was characterized as a nodular melanoma, Clark's level 3, Breslow's depth 2.0 mm. One out of 14 inguinal nodes, excised in a subsequent operation, was found to be invaded. She received 6 cycles of chemotherapy (cis-platin-dacarbazine) and remained disease-free for 15 months. The cytologic examination of the ascitic aspiration confirmed the presence of malignant cells positive for the melanocyte specific antibody S-100 (Figure. 1). The patient refused to be operated, gave her written consent for further research on the specimens obtained, and expired 40 days after presentation.

Bottom Line: Interestingly this tumor had no detectable telomerase activity as indicated by the Telomere Repeat Amplification Protocol.Telomeric Flow-FISH and quantitative telomeric FISH on mitotic preparations showed that malignant cells had relatively short telomeres.Microsatellite instability was ruled out by the allelic pattern of two major mononucleotide repeats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Genetics, Biomedical Research Foundation of the Academy of Athens, Greece (BRFAA). sgagos@bioacademy.gr.

ABSTRACT
Malignant melanomas are characterized by increased karyotypic complexity, extended aneuploidy and heteroploidy. We report a melanoma metastasis to the peritoneal cavity with an exceptionally stable, abnormal pseudodiploid karyotype as verified by G-Banding, subtelomeric, centromeric and quantitative Fluorescence in Situ Hybridization (FISH). Interestingly this tumor had no detectable telomerase activity as indicated by the Telomere Repeat Amplification Protocol. Telomeric Flow-FISH and quantitative telomeric FISH on mitotic preparations showed that malignant cells had relatively short telomeres. Microsatellite instability was ruled out by the allelic pattern of two major mononucleotide repeats. Our data suggest that a combination of melanoma specific genomic imbalances were sufficient and enough for this fatal tumor progression, that was not accompanied by genomic instability, telomerase activity, or the engagement of the alternative recombinatorial telomere lengthening pathway.

No MeSH data available.


Related in: MedlinePlus