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The prevention of endothelial dysfunction through endothelial cell apoptosis inhibition in a hypercholesterolemic rabbit model: the effect of L-arginine supplementation.

Nematbakhsh M, Haghjooyjavanmard S, Mahmoodi F, Monajemi AR - Lipids Health Dis (2008)

Bottom Line: No significant differences were observed in cholesterol level between two groups, but the nitrite concentration in L-arginine group was significantly higher than other group (control group: 11.8 +/- 1; L-arginine group: 14.7 +/- 0.5 micromol/l); (p < 0.05).The number of intimal apoptotic cells/500 cells of aorta in two groups of experiment were statistically different (control group: 39.3 +/- 7.6; L-arginine group: 21.5 +/- 5.3) (p < 0.05).The inhibition of endothelial cells apoptosis by L-arginine restores endothelial function in a model of hypercholesterolemia.

View Article: PubMed Central - HTML - PubMed

Affiliation: Deparment of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran. nematbakhsh@gmail.com

ABSTRACT

Background: The impact of L-arginine on atherogenesis and its ability to prevent endothelial dysfunction have been studied extensively during the past years. L-arginine is a substance for nitric oxide synthesis which involves in apoptosis. Hypercholesterolemia promotes endothelial dysfunction, and it is hypothesized that L-arginine prevents endothelial dysfunction through endothelial cells apoptosis inhibition. To test this hypothesis, thirty rabbits were assigned into two groups. The control group received 1% cholesterol diet for 4 weeks, and the L-arginine group received same diets plus 3% L-arginine in drinking water.

Results: No significant differences were observed in cholesterol level between two groups, but the nitrite concentration in L-arginine group was significantly higher than other group (control group: 11.8 +/- 1; L-arginine group: 14.7 +/- 0.5 micromol/l); (p < 0.05). The aorta score of fatty streak in control group was 0.875 +/- 0.35, but no fatty streak lesion was detected in L-arginine group (p < 0.05). The number of intimal apoptotic cells/500 cells of aorta in two groups of experiment were statistically different (control group: 39.3 +/- 7.6; L-arginine group: 21.5 +/- 5.3) (p < 0.05).

Conclusion: The inhibition of endothelial cells apoptosis by L-arginine restores endothelial function in a model of hypercholesterolemia.

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TUNEL performed in rabbit's aorta. Aorta section from rabbit that apoptosis of arterial cells is a prominent feature of atherosclerotic lesions (control group), but less apoptotic cells were observed in the aorta of L-arginine group.
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Figure 2: TUNEL performed in rabbit's aorta. Aorta section from rabbit that apoptosis of arterial cells is a prominent feature of atherosclerotic lesions (control group), but less apoptotic cells were observed in the aorta of L-arginine group.

Mentions: The number of intimal apoptotic cells/500 cells in rabbit's aortas is demonstrated in figure 1 (control group: 39.3 ± 7.6, L-arginine group: 21.5 ± 5.3) (p < 0.05). In situ detection of apoptotic cells indicate that in aorta section from control group, the apoptosis of intimal cells is a prominent feature of atherosclerotic lesions, but less apoptosis cells were observed in L-arginine group (figure 2).


The prevention of endothelial dysfunction through endothelial cell apoptosis inhibition in a hypercholesterolemic rabbit model: the effect of L-arginine supplementation.

Nematbakhsh M, Haghjooyjavanmard S, Mahmoodi F, Monajemi AR - Lipids Health Dis (2008)

TUNEL performed in rabbit's aorta. Aorta section from rabbit that apoptosis of arterial cells is a prominent feature of atherosclerotic lesions (control group), but less apoptotic cells were observed in the aorta of L-arginine group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2533308&req=5

Figure 2: TUNEL performed in rabbit's aorta. Aorta section from rabbit that apoptosis of arterial cells is a prominent feature of atherosclerotic lesions (control group), but less apoptotic cells were observed in the aorta of L-arginine group.
Mentions: The number of intimal apoptotic cells/500 cells in rabbit's aortas is demonstrated in figure 1 (control group: 39.3 ± 7.6, L-arginine group: 21.5 ± 5.3) (p < 0.05). In situ detection of apoptotic cells indicate that in aorta section from control group, the apoptosis of intimal cells is a prominent feature of atherosclerotic lesions, but less apoptosis cells were observed in L-arginine group (figure 2).

Bottom Line: No significant differences were observed in cholesterol level between two groups, but the nitrite concentration in L-arginine group was significantly higher than other group (control group: 11.8 +/- 1; L-arginine group: 14.7 +/- 0.5 micromol/l); (p < 0.05).The number of intimal apoptotic cells/500 cells of aorta in two groups of experiment were statistically different (control group: 39.3 +/- 7.6; L-arginine group: 21.5 +/- 5.3) (p < 0.05).The inhibition of endothelial cells apoptosis by L-arginine restores endothelial function in a model of hypercholesterolemia.

View Article: PubMed Central - HTML - PubMed

Affiliation: Deparment of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran. nematbakhsh@gmail.com

ABSTRACT

Background: The impact of L-arginine on atherogenesis and its ability to prevent endothelial dysfunction have been studied extensively during the past years. L-arginine is a substance for nitric oxide synthesis which involves in apoptosis. Hypercholesterolemia promotes endothelial dysfunction, and it is hypothesized that L-arginine prevents endothelial dysfunction through endothelial cells apoptosis inhibition. To test this hypothesis, thirty rabbits were assigned into two groups. The control group received 1% cholesterol diet for 4 weeks, and the L-arginine group received same diets plus 3% L-arginine in drinking water.

Results: No significant differences were observed in cholesterol level between two groups, but the nitrite concentration in L-arginine group was significantly higher than other group (control group: 11.8 +/- 1; L-arginine group: 14.7 +/- 0.5 micromol/l); (p < 0.05). The aorta score of fatty streak in control group was 0.875 +/- 0.35, but no fatty streak lesion was detected in L-arginine group (p < 0.05). The number of intimal apoptotic cells/500 cells of aorta in two groups of experiment were statistically different (control group: 39.3 +/- 7.6; L-arginine group: 21.5 +/- 5.3) (p < 0.05).

Conclusion: The inhibition of endothelial cells apoptosis by L-arginine restores endothelial function in a model of hypercholesterolemia.

Show MeSH
Related in: MedlinePlus