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Knockout mice reveal a role for protein tyrosine phosphatase H1 in cognition.

Patrignani C, Magnone MC, Tavano P, Ardizzone M, Muzio V, Gréco B, Zaratin PF - Behav Brain Funct (2008)

Bottom Line: PTPH1 expression pattern was evaluated by LacZ staining in the brain and PTPH1-KO and WT mice (n = 10 per gender per genotype) were also behaviorally tested for CNS functions.In CNS, PTPH1 is expressed during development and in adulthood and mainly localized in hippocampus, thalamus, cortex and cerebellum neurons.These results demonstrate for the first time a neuronal expression of PTPH1 and its functionality at the level of cognition.

View Article: PubMed Central - HTML - PubMed

Affiliation: MerckSerono Ivrea, Colleretto G, (TO) 10010, Italy. claudia.patrignani@merckserono.net.

ABSTRACT

Background: The present study has investigated the protein tyrosine phosphatase H1 (PTPH1) expression pattern in mouse brain and its impact on CNS functions.

Methods: We have previously described a PTPH1-KO mouse, generated by replacing the PTP catalytic and the PDZ domain with a LacZ neomycin cassette. PTPH1 expression pattern was evaluated by LacZ staining in the brain and PTPH1-KO and WT mice (n = 10 per gender per genotype) were also behaviorally tested for CNS functions.

Results: In CNS, PTPH1 is expressed during development and in adulthood and mainly localized in hippocampus, thalamus, cortex and cerebellum neurons. The behavioral tests performed on the PTPH1-KO mice showed an impact on working memory in male mice and an impaired learning performance at rotarod in females.

Conclusion: These results demonstrate for the first time a neuronal expression of PTPH1 and its functionality at the level of cognition.

No MeSH data available.


PTPH1-KO cerebral cortex. a: colocalization of NeuN-ir and LacZ staining signal in the Rc (100×, scale bar: 4.5 μm).; b: detail of the cytoplasmatic signal of LacZ in neurons.
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Figure 4: PTPH1-KO cerebral cortex. a: colocalization of NeuN-ir and LacZ staining signal in the Rc (100×, scale bar: 4.5 μm).; b: detail of the cytoplasmatic signal of LacZ in neurons.

Mentions: In cortical regions, LacZ was expressed in the external pyramidal (III) and internal granular layer (IVA) of the cerebral cortex (Figures 2a, 2b), in the retrosplenial cortex (Figures. 3a, 3b 3c, 4a and 4b) and indusium griseum (Figures 3a, 3b). In the cerebellum, in spite of a strong staining in the whole mount (Figure 2), only a faint LacZ signal was observed in sections (Figures 5a, 5b) in particular in the granule cells, close to the nuclei. The RT-PCR on cortical and cerebellar extracts confirmed the presence of LacZ expression in these brain areas (Figure 6).


Knockout mice reveal a role for protein tyrosine phosphatase H1 in cognition.

Patrignani C, Magnone MC, Tavano P, Ardizzone M, Muzio V, Gréco B, Zaratin PF - Behav Brain Funct (2008)

PTPH1-KO cerebral cortex. a: colocalization of NeuN-ir and LacZ staining signal in the Rc (100×, scale bar: 4.5 μm).; b: detail of the cytoplasmatic signal of LacZ in neurons.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2531118&req=5

Figure 4: PTPH1-KO cerebral cortex. a: colocalization of NeuN-ir and LacZ staining signal in the Rc (100×, scale bar: 4.5 μm).; b: detail of the cytoplasmatic signal of LacZ in neurons.
Mentions: In cortical regions, LacZ was expressed in the external pyramidal (III) and internal granular layer (IVA) of the cerebral cortex (Figures 2a, 2b), in the retrosplenial cortex (Figures. 3a, 3b 3c, 4a and 4b) and indusium griseum (Figures 3a, 3b). In the cerebellum, in spite of a strong staining in the whole mount (Figure 2), only a faint LacZ signal was observed in sections (Figures 5a, 5b) in particular in the granule cells, close to the nuclei. The RT-PCR on cortical and cerebellar extracts confirmed the presence of LacZ expression in these brain areas (Figure 6).

Bottom Line: PTPH1 expression pattern was evaluated by LacZ staining in the brain and PTPH1-KO and WT mice (n = 10 per gender per genotype) were also behaviorally tested for CNS functions.In CNS, PTPH1 is expressed during development and in adulthood and mainly localized in hippocampus, thalamus, cortex and cerebellum neurons.These results demonstrate for the first time a neuronal expression of PTPH1 and its functionality at the level of cognition.

View Article: PubMed Central - HTML - PubMed

Affiliation: MerckSerono Ivrea, Colleretto G, (TO) 10010, Italy. claudia.patrignani@merckserono.net.

ABSTRACT

Background: The present study has investigated the protein tyrosine phosphatase H1 (PTPH1) expression pattern in mouse brain and its impact on CNS functions.

Methods: We have previously described a PTPH1-KO mouse, generated by replacing the PTP catalytic and the PDZ domain with a LacZ neomycin cassette. PTPH1 expression pattern was evaluated by LacZ staining in the brain and PTPH1-KO and WT mice (n = 10 per gender per genotype) were also behaviorally tested for CNS functions.

Results: In CNS, PTPH1 is expressed during development and in adulthood and mainly localized in hippocampus, thalamus, cortex and cerebellum neurons. The behavioral tests performed on the PTPH1-KO mice showed an impact on working memory in male mice and an impaired learning performance at rotarod in females.

Conclusion: These results demonstrate for the first time a neuronal expression of PTPH1 and its functionality at the level of cognition.

No MeSH data available.