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Herbex-kid Inhibits Immediate Hypersensitivity Reactions in Mice and Rats.

Kumar A, Prasad R, Jogge NM, Bhojraj S, Emerson SF, Prabakar S - Evid Based Complement Alternat Med (2008)

Bottom Line: In C 48/80-induced skin itch model, all dose levels of HK significantly (P < 0.001) decreased the time spent in itching and the number of itches.HK did not produce any significant inhibition in histamine induced contraction in guinea pig ileum.From the above findings we conclude that the HK possesses antiallergic activity mediated by reducing of the release mediators from mast cells and also by 5-HT antagonism without the involvement of histamine (H1) receptors.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, TIFAC CORE in Herbal Drugs, JSS College of Pharmacy, Ootacamund 643001 and Green Milk Concepts, Herbal Division of Apex Laboratories, Chennai 600038, India.

ABSTRACT
Herbex-kid (HK), a polyherbal formulation was evaluated in various experimental allergic models of Type I hypersensitivity reactions. Compound 48/80 (C 48/80) has been shown to induce rat mesentery mast cell degranulation and HK (1.07, 10.75 and 107.5 mg ml(-1)) inhibited the mast cell degranulation in a dose dependent manner. HK (1.07, 10.75 and 107.5 mg kg(-1); p.o.) showed dose-dependent protection against C 48/80 induced systemic anaphylaxis in male Balb/C mice. In active anaphylaxis model, male Wistar rats orally administered with 10.75 and 107.5 mg kg(-1) of HK showed significant (P < 0.01) protection against mast cell degranulation, while in passive anaphylaxis model, only at 107.5 mg kg(-1) showed significant (P < 0.01) reduction in mast cell degranulation. HK at all dose levels was able to significantly decrease the time spent in nasal rubbing in Wistar rats sensitized to ovalbumin, while only at 107.5 mg kg(-1) it showed significant (P < 0.01) reduction in number of sneezes. In C 48/80-induced skin itch model, all dose levels of HK significantly (P < 0.001) decreased the time spent in itching and the number of itches. HK did not produce any significant inhibition in histamine induced contraction in guinea pig ileum. From the above findings we conclude that the HK possesses antiallergic activity mediated by reducing of the release mediators from mast cells and also by 5-HT antagonism without the involvement of histamine (H1) receptors.

No MeSH data available.


Related in: MedlinePlus

Effect of Herbex-kid (HK) in C 48/80 induced systemic anaphylaxis in Balb/C mice. Each bar represents mean ± SEM of six mice each. **P < 0.01 vs control. One way ANOVA followed by Dunnett's multiple comparison test. C 48/80: Compound 48/80.
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Figure 2: Effect of Herbex-kid (HK) in C 48/80 induced systemic anaphylaxis in Balb/C mice. Each bar represents mean ± SEM of six mice each. **P < 0.01 vs control. One way ANOVA followed by Dunnett's multiple comparison test. C 48/80: Compound 48/80.

Mentions: In vehicle treated mice, systemic anaphylaxis was observed within 19.3 ± 1.5 min. Oral pre-treatment with HK, 1 h before the administration of C 48/80, showed a dose-dependent increase in latency to death time. Only HK at 107.5 mg kg−1 produced significant increase (P < 0.01) in latency to death time, namely, 49.5 ± 9.6 min when compared with controls (Fig. 2).Figure 2.


Herbex-kid Inhibits Immediate Hypersensitivity Reactions in Mice and Rats.

Kumar A, Prasad R, Jogge NM, Bhojraj S, Emerson SF, Prabakar S - Evid Based Complement Alternat Med (2008)

Effect of Herbex-kid (HK) in C 48/80 induced systemic anaphylaxis in Balb/C mice. Each bar represents mean ± SEM of six mice each. **P < 0.01 vs control. One way ANOVA followed by Dunnett's multiple comparison test. C 48/80: Compound 48/80.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2529394&req=5

Figure 2: Effect of Herbex-kid (HK) in C 48/80 induced systemic anaphylaxis in Balb/C mice. Each bar represents mean ± SEM of six mice each. **P < 0.01 vs control. One way ANOVA followed by Dunnett's multiple comparison test. C 48/80: Compound 48/80.
Mentions: In vehicle treated mice, systemic anaphylaxis was observed within 19.3 ± 1.5 min. Oral pre-treatment with HK, 1 h before the administration of C 48/80, showed a dose-dependent increase in latency to death time. Only HK at 107.5 mg kg−1 produced significant increase (P < 0.01) in latency to death time, namely, 49.5 ± 9.6 min when compared with controls (Fig. 2).Figure 2.

Bottom Line: In C 48/80-induced skin itch model, all dose levels of HK significantly (P < 0.001) decreased the time spent in itching and the number of itches.HK did not produce any significant inhibition in histamine induced contraction in guinea pig ileum.From the above findings we conclude that the HK possesses antiallergic activity mediated by reducing of the release mediators from mast cells and also by 5-HT antagonism without the involvement of histamine (H1) receptors.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, TIFAC CORE in Herbal Drugs, JSS College of Pharmacy, Ootacamund 643001 and Green Milk Concepts, Herbal Division of Apex Laboratories, Chennai 600038, India.

ABSTRACT
Herbex-kid (HK), a polyherbal formulation was evaluated in various experimental allergic models of Type I hypersensitivity reactions. Compound 48/80 (C 48/80) has been shown to induce rat mesentery mast cell degranulation and HK (1.07, 10.75 and 107.5 mg ml(-1)) inhibited the mast cell degranulation in a dose dependent manner. HK (1.07, 10.75 and 107.5 mg kg(-1); p.o.) showed dose-dependent protection against C 48/80 induced systemic anaphylaxis in male Balb/C mice. In active anaphylaxis model, male Wistar rats orally administered with 10.75 and 107.5 mg kg(-1) of HK showed significant (P < 0.01) protection against mast cell degranulation, while in passive anaphylaxis model, only at 107.5 mg kg(-1) showed significant (P < 0.01) reduction in mast cell degranulation. HK at all dose levels was able to significantly decrease the time spent in nasal rubbing in Wistar rats sensitized to ovalbumin, while only at 107.5 mg kg(-1) it showed significant (P < 0.01) reduction in number of sneezes. In C 48/80-induced skin itch model, all dose levels of HK significantly (P < 0.001) decreased the time spent in itching and the number of itches. HK did not produce any significant inhibition in histamine induced contraction in guinea pig ileum. From the above findings we conclude that the HK possesses antiallergic activity mediated by reducing of the release mediators from mast cells and also by 5-HT antagonism without the involvement of histamine (H1) receptors.

No MeSH data available.


Related in: MedlinePlus