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Brazilian propolis: correlation between chemical composition and antimicrobial activity.

Salomão K, Pereira PR, Campos LC, Borba CM, Cabello PH, Marcucci MC, de Castro SL - Evid Based Complement Alternat Med (2008)

Bottom Line: Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) and of trypomastigotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4) and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN).When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliable results.For group C no association was observed between the anitmicrobial effect and any component of the extracts.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ultra-estrutura e Biologia Celular, Departamento de Bacteriologia, Departamento de Micologia, Departamento de Genética, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro and Pós-graduação em Farmácia, Núcleo de Pós-graduação, Pesquisa e Extensão, Universidade Bandeirante de São Paulo, São Paulo, Brazil.

ABSTRACT
The chemical composition of ethanol extracts from samples of Brazilian propolis (EEPs) determined by HPLC and their activity against Trypanosoma cruzi, Staphylococcus aureus, Streptococcus pneumoniae, Klebisiella pneumoniae, Candida albicans, Sporothrix schenckii and Paracoccidioides brasiliensis were determined. Based on the predominant botanical origin in the region of samples' collection, the 10 extracts were separated into three groups: A (B. dracunculifolia + Auraucaria spp), B (B. dracunculifolia) and C (Araucaria spp). Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) and of trypomastigotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4) and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN). When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliable results. However, an overall analysis revealed for group A an association of S. aureus with caffeic acid (CAF) and dicaffeoylquinic acid 3 (CAFQ3), of S. pneumoniae with CAFQ3 and monocaffeoylquinic acid 2 (CAFQ2) and of T. cruzi also with CAFQ3. For group B, a higher activity against S. pneumoniae was associated DCBEN and for T. cruzi with CAF. For group C no association was observed between the anitmicrobial effect and any component of the extracts. The present study reinforces the relevance of PCUM and derivatives, especially prenylated ones and also of caffeolyquinic acids, on the biological activity of Brazilian propolis.

No MeSH data available.


Related in: MedlinePlus

Bactericidal activity of Brazilian propolis extracts expressed as MIC values in µg ml−1: (a) Streptococcus pneumoniae; (b) Staphylococcus aureus.
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Figure 3: Bactericidal activity of Brazilian propolis extracts expressed as MIC values in µg ml−1: (a) Streptococcus pneumoniae; (b) Staphylococcus aureus.

Mentions: The MIC range for S. pneumoniae was 0.2–0.8 μg ml−1 and for S. aureus, 1.6–52.4 μg ml−1 (Fig. 3). All the extracts were inactive against K. pneumoniae. Analysis by the method of multiple regression of all the extracts together showed for S. aureus a positive correlation for p-coumaric acid (PCUM) (P = 0.0002) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) (P = 0.006), meaning higher concentrations of each compound led to higher bactericidal effect and a negative one for 3-methoxy-4-hydroxycinnamaldehyde (G2) (P = 0.001) and vanillin (VAN) (P = 0.026). While for S. pneumoniae, a negative correlation between the activity and the concentration of G2 (P = 0.024) was observed. When the same approach was employed for each group, since the number of extracts was four, three and three, for groups A, B and C, respectively, the multiple regression analysis gave unreliable results. However, an overall analysis revealed for group A (B. dracunculifolia + Auraucaria spp) there was an association of concentrations of caffeic acid (CAF) and dicaffeoylquinic acid 3 (CAFQ3) with the activity against S. aureus, while for S. pneumoniae such association occurred and between CAFQ3 and monocaffeoylquinic acid 2 (CAFQ2). In group B (B. dracunculifolia) higher activity against S. pneumoniae was associated with 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN). For group C (Araucaria spp) no association was observed between the bactericidal effect and any component of the extracts.Figure 3.


Brazilian propolis: correlation between chemical composition and antimicrobial activity.

Salomão K, Pereira PR, Campos LC, Borba CM, Cabello PH, Marcucci MC, de Castro SL - Evid Based Complement Alternat Med (2008)

Bactericidal activity of Brazilian propolis extracts expressed as MIC values in µg ml−1: (a) Streptococcus pneumoniae; (b) Staphylococcus aureus.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2529390&req=5

Figure 3: Bactericidal activity of Brazilian propolis extracts expressed as MIC values in µg ml−1: (a) Streptococcus pneumoniae; (b) Staphylococcus aureus.
Mentions: The MIC range for S. pneumoniae was 0.2–0.8 μg ml−1 and for S. aureus, 1.6–52.4 μg ml−1 (Fig. 3). All the extracts were inactive against K. pneumoniae. Analysis by the method of multiple regression of all the extracts together showed for S. aureus a positive correlation for p-coumaric acid (PCUM) (P = 0.0002) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) (P = 0.006), meaning higher concentrations of each compound led to higher bactericidal effect and a negative one for 3-methoxy-4-hydroxycinnamaldehyde (G2) (P = 0.001) and vanillin (VAN) (P = 0.026). While for S. pneumoniae, a negative correlation between the activity and the concentration of G2 (P = 0.024) was observed. When the same approach was employed for each group, since the number of extracts was four, three and three, for groups A, B and C, respectively, the multiple regression analysis gave unreliable results. However, an overall analysis revealed for group A (B. dracunculifolia + Auraucaria spp) there was an association of concentrations of caffeic acid (CAF) and dicaffeoylquinic acid 3 (CAFQ3) with the activity against S. aureus, while for S. pneumoniae such association occurred and between CAFQ3 and monocaffeoylquinic acid 2 (CAFQ2). In group B (B. dracunculifolia) higher activity against S. pneumoniae was associated with 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN). For group C (Araucaria spp) no association was observed between the bactericidal effect and any component of the extracts.Figure 3.

Bottom Line: Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) and of trypomastigotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4) and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN).When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliable results.For group C no association was observed between the anitmicrobial effect and any component of the extracts.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ultra-estrutura e Biologia Celular, Departamento de Bacteriologia, Departamento de Micologia, Departamento de Genética, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro and Pós-graduação em Farmácia, Núcleo de Pós-graduação, Pesquisa e Extensão, Universidade Bandeirante de São Paulo, São Paulo, Brazil.

ABSTRACT
The chemical composition of ethanol extracts from samples of Brazilian propolis (EEPs) determined by HPLC and their activity against Trypanosoma cruzi, Staphylococcus aureus, Streptococcus pneumoniae, Klebisiella pneumoniae, Candida albicans, Sporothrix schenckii and Paracoccidioides brasiliensis were determined. Based on the predominant botanical origin in the region of samples' collection, the 10 extracts were separated into three groups: A (B. dracunculifolia + Auraucaria spp), B (B. dracunculifolia) and C (Araucaria spp). Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) and of trypomastigotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4) and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN). When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliable results. However, an overall analysis revealed for group A an association of S. aureus with caffeic acid (CAF) and dicaffeoylquinic acid 3 (CAFQ3), of S. pneumoniae with CAFQ3 and monocaffeoylquinic acid 2 (CAFQ2) and of T. cruzi also with CAFQ3. For group B, a higher activity against S. pneumoniae was associated DCBEN and for T. cruzi with CAF. For group C no association was observed between the anitmicrobial effect and any component of the extracts. The present study reinforces the relevance of PCUM and derivatives, especially prenylated ones and also of caffeolyquinic acids, on the biological activity of Brazilian propolis.

No MeSH data available.


Related in: MedlinePlus