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Brazilian propolis: correlation between chemical composition and antimicrobial activity.

Salomão K, Pereira PR, Campos LC, Borba CM, Cabello PH, Marcucci MC, de Castro SL - Evid Based Complement Alternat Med (2008)

Bottom Line: Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) and of trypomastigotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4) and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN).When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliable results.For group C no association was observed between the anitmicrobial effect and any component of the extracts.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ultra-estrutura e Biologia Celular, Departamento de Bacteriologia, Departamento de Micologia, Departamento de Genética, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro and Pós-graduação em Farmácia, Núcleo de Pós-graduação, Pesquisa e Extensão, Universidade Bandeirante de São Paulo, São Paulo, Brazil.

ABSTRACT
The chemical composition of ethanol extracts from samples of Brazilian propolis (EEPs) determined by HPLC and their activity against Trypanosoma cruzi, Staphylococcus aureus, Streptococcus pneumoniae, Klebisiella pneumoniae, Candida albicans, Sporothrix schenckii and Paracoccidioides brasiliensis were determined. Based on the predominant botanical origin in the region of samples' collection, the 10 extracts were separated into three groups: A (B. dracunculifolia + Auraucaria spp), B (B. dracunculifolia) and C (Araucaria spp). Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) and of trypomastigotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4) and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN). When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliable results. However, an overall analysis revealed for group A an association of S. aureus with caffeic acid (CAF) and dicaffeoylquinic acid 3 (CAFQ3), of S. pneumoniae with CAFQ3 and monocaffeoylquinic acid 2 (CAFQ2) and of T. cruzi also with CAFQ3. For group B, a higher activity against S. pneumoniae was associated DCBEN and for T. cruzi with CAF. For group C no association was observed between the anitmicrobial effect and any component of the extracts. The present study reinforces the relevance of PCUM and derivatives, especially prenylated ones and also of caffeolyquinic acids, on the biological activity of Brazilian propolis.

No MeSH data available.


Related in: MedlinePlus

Concentration expressed in mg g−1 dried extract (mean ± standard deviation) of individual components of the ethanol extracts of Brazilian propolis samples separated by botanical origin: (A) group A; (B) group B; (C) group C.
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Figure 1: Concentration expressed in mg g−1 dried extract (mean ± standard deviation) of individual components of the ethanol extracts of Brazilian propolis samples separated by botanical origin: (A) group A; (B) group B; (C) group C.

Mentions: The chemical composition of each EEP quantified by HPLC is presented in Table 1. In order to better visualize the distribution of the components in groups A, B and C based on the predominant vegetation the concentration of each component was included in each group expressed as mean ± standard deviation (Fig. 1).Figure 1.


Brazilian propolis: correlation between chemical composition and antimicrobial activity.

Salomão K, Pereira PR, Campos LC, Borba CM, Cabello PH, Marcucci MC, de Castro SL - Evid Based Complement Alternat Med (2008)

Concentration expressed in mg g−1 dried extract (mean ± standard deviation) of individual components of the ethanol extracts of Brazilian propolis samples separated by botanical origin: (A) group A; (B) group B; (C) group C.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2529390&req=5

Figure 1: Concentration expressed in mg g−1 dried extract (mean ± standard deviation) of individual components of the ethanol extracts of Brazilian propolis samples separated by botanical origin: (A) group A; (B) group B; (C) group C.
Mentions: The chemical composition of each EEP quantified by HPLC is presented in Table 1. In order to better visualize the distribution of the components in groups A, B and C based on the predominant vegetation the concentration of each component was included in each group expressed as mean ± standard deviation (Fig. 1).Figure 1.

Bottom Line: Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) and of trypomastigotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4) and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN).When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliable results.For group C no association was observed between the anitmicrobial effect and any component of the extracts.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ultra-estrutura e Biologia Celular, Departamento de Bacteriologia, Departamento de Micologia, Departamento de Genética, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro and Pós-graduação em Farmácia, Núcleo de Pós-graduação, Pesquisa e Extensão, Universidade Bandeirante de São Paulo, São Paulo, Brazil.

ABSTRACT
The chemical composition of ethanol extracts from samples of Brazilian propolis (EEPs) determined by HPLC and their activity against Trypanosoma cruzi, Staphylococcus aureus, Streptococcus pneumoniae, Klebisiella pneumoniae, Candida albicans, Sporothrix schenckii and Paracoccidioides brasiliensis were determined. Based on the predominant botanical origin in the region of samples' collection, the 10 extracts were separated into three groups: A (B. dracunculifolia + Auraucaria spp), B (B. dracunculifolia) and C (Araucaria spp). Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM) and 3-(4-hydroxy-3-(oxo-butenyl)-phenylacrylic acid (DHCA1) and of trypomastigotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4) and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN). When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliable results. However, an overall analysis revealed for group A an association of S. aureus with caffeic acid (CAF) and dicaffeoylquinic acid 3 (CAFQ3), of S. pneumoniae with CAFQ3 and monocaffeoylquinic acid 2 (CAFQ2) and of T. cruzi also with CAFQ3. For group B, a higher activity against S. pneumoniae was associated DCBEN and for T. cruzi with CAF. For group C no association was observed between the anitmicrobial effect and any component of the extracts. The present study reinforces the relevance of PCUM and derivatives, especially prenylated ones and also of caffeolyquinic acids, on the biological activity of Brazilian propolis.

No MeSH data available.


Related in: MedlinePlus