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Complete genome of Phenylobacterium zucineum--a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562.

Luo Y, Xu X, Ding Z, Liu Z, Zhang B, Yan Z, Sun J, Hu S, Hu X - BMC Genomics (2008)

Bottom Line: Notably, P. zucineum has a gene that is strikingly similar, both structurally and functionally, to the cell cycle master regulator CtrA of C. crescentus, and most of the genes directly regulated by CtrA in the latter have orthologs in the former.This work presents the first complete bacterial genome in the genus Phenylobacterium.Comparative genomic analysis indicated that the CtrA regulon is well conserved between C. crescentus and P. zucineum.

View Article: PubMed Central - HTML - PubMed

Affiliation: Cancer Institute, (Key Laboratory for Cancer Intervention and Prevention, Key Laboratory of Molecular Biology in Medical Sciences), Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, PR China. luoyf@big.ac.cn

ABSTRACT

Background: Phenylobacterium zucineum is a recently identified facultative intracellular species isolated from the human leukemia cell line K562. Unlike the known intracellular pathogens, P. zucineum maintains a stable association with its host cell without affecting the growth and morphology of the latter.

Results: Here, we report the whole genome sequence of the type strain HLK1T. The genome consists of a circular chromosome (3,996,255 bp) and a circular plasmid (382,976 bp). It encodes 3,861 putative proteins, 42 tRNAs, and a 16S-23S-5S rRNA operon. Comparative genomic analysis revealed that it is phylogenetically closest to Caulobacter crescentus, a model species for cell cycle research. Notably, P. zucineum has a gene that is strikingly similar, both structurally and functionally, to the cell cycle master regulator CtrA of C. crescentus, and most of the genes directly regulated by CtrA in the latter have orthologs in the former.

Conclusion: This work presents the first complete bacterial genome in the genus Phenylobacterium. Comparative genomic analysis indicated that the CtrA regulon is well conserved between C. crescentus and P. zucineum.

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List of top 10 complete sequenced bacteria closest to P. zucineum. All 10 are alphaproteobacteria. Among all the sequenced bacterial genomes, C. crescentus shares the greatest number of similar ORFs with P. zucineum
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Figure 4: List of top 10 complete sequenced bacteria closest to P. zucineum. All 10 are alphaproteobacteria. Among all the sequenced bacterial genomes, C. crescentus shares the greatest number of similar ORFs with P. zucineum

Mentions: Comparative genomic analysis demonstrated that P. zucineum is phylogenetically the closest to C. crescentus [18] (Figure 4), consistent with the phylogenetic analysis based on 16S RNA gene sequences (Figure 5).


Complete genome of Phenylobacterium zucineum--a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562.

Luo Y, Xu X, Ding Z, Liu Z, Zhang B, Yan Z, Sun J, Hu S, Hu X - BMC Genomics (2008)

List of top 10 complete sequenced bacteria closest to P. zucineum. All 10 are alphaproteobacteria. Among all the sequenced bacterial genomes, C. crescentus shares the greatest number of similar ORFs with P. zucineum
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2529317&req=5

Figure 4: List of top 10 complete sequenced bacteria closest to P. zucineum. All 10 are alphaproteobacteria. Among all the sequenced bacterial genomes, C. crescentus shares the greatest number of similar ORFs with P. zucineum
Mentions: Comparative genomic analysis demonstrated that P. zucineum is phylogenetically the closest to C. crescentus [18] (Figure 4), consistent with the phylogenetic analysis based on 16S RNA gene sequences (Figure 5).

Bottom Line: Notably, P. zucineum has a gene that is strikingly similar, both structurally and functionally, to the cell cycle master regulator CtrA of C. crescentus, and most of the genes directly regulated by CtrA in the latter have orthologs in the former.This work presents the first complete bacterial genome in the genus Phenylobacterium.Comparative genomic analysis indicated that the CtrA regulon is well conserved between C. crescentus and P. zucineum.

View Article: PubMed Central - HTML - PubMed

Affiliation: Cancer Institute, (Key Laboratory for Cancer Intervention and Prevention, Key Laboratory of Molecular Biology in Medical Sciences), Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, PR China. luoyf@big.ac.cn

ABSTRACT

Background: Phenylobacterium zucineum is a recently identified facultative intracellular species isolated from the human leukemia cell line K562. Unlike the known intracellular pathogens, P. zucineum maintains a stable association with its host cell without affecting the growth and morphology of the latter.

Results: Here, we report the whole genome sequence of the type strain HLK1T. The genome consists of a circular chromosome (3,996,255 bp) and a circular plasmid (382,976 bp). It encodes 3,861 putative proteins, 42 tRNAs, and a 16S-23S-5S rRNA operon. Comparative genomic analysis revealed that it is phylogenetically closest to Caulobacter crescentus, a model species for cell cycle research. Notably, P. zucineum has a gene that is strikingly similar, both structurally and functionally, to the cell cycle master regulator CtrA of C. crescentus, and most of the genes directly regulated by CtrA in the latter have orthologs in the former.

Conclusion: This work presents the first complete bacterial genome in the genus Phenylobacterium. Comparative genomic analysis indicated that the CtrA regulon is well conserved between C. crescentus and P. zucineum.

Show MeSH
Related in: MedlinePlus