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Prognostic value analysis of urokinase-type plasminogen activator receptor in oral squamous cell carcinoma: an immunohistochemical study.

Bacchiocchi R, Rubini C, Pierpaoli E, Borghetti G, Procacci P, Nocini PF, Santarelli A, Rocchetti R, Ciavarella D, Lo Muzio L, Fazioli F - BMC Cancer (2008)

Bottom Line: Among tumour progression biomarkers, already known for their involvement in other neoplasia, a crucial role is ascribed to the urokinase-type plasminogen activator receptor (uPAR), which plays a multiple role in extracellular proteolysis, cell migration and tissue remodelling not only as a receptor for the zymogen pro-uPA but also as a component for cell adhesion and as a chemoattractant.The purpose of this study was to gain information on the expression of uPAR in OSCC and to verify whether this molecule can have a role as a prognostic/predictive marker for this neoplasia.The results obtained in this study suggest a role of uPAR as a potential biomarker useful to identify higher risk subgroups of OSCC patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Molecular Pathology and Innovative Therapies, Polytechnic University of the Marche Region, Ancona, Italy. bacchiocchi@univpm.it

ABSTRACT

Background: Oral squamous cell carcinoma (OSCC) represents the most common oral malignancy. Despite recent advances in therapy, up to 50% of the cases have relapse and/or metastasis. There is therefore a strong need for the identification of new biological markers able to predict the clinical behaviour of these lesions in order to improve quality of life and overall survival. Among tumour progression biomarkers, already known for their involvement in other neoplasia, a crucial role is ascribed to the urokinase-type plasminogen activator receptor (uPAR), which plays a multiple role in extracellular proteolysis, cell migration and tissue remodelling not only as a receptor for the zymogen pro-uPA but also as a component for cell adhesion and as a chemoattractant. The purpose of this study was to gain information on the expression of uPAR in OSCC and to verify whether this molecule can have a role as a prognostic/predictive marker for this neoplasia.

Methods: In a retrospective study, a cohort of 189 OSCC patients was investigated for uPAR expression and its cellular localization by immunohistochemistry. As standard controls, 8 normal oral mucosal tissues free of malignancy, obtained from patients with no evidence or history of oral cavity tumours, were similarly investigated. After grouping for uPAR expression, OSCCs were statistically analyzed for the variables age, gender, histological grading (G), tumour size, recurrence, TNM staging and overall survival rate.

Results: In our immunohistochemical study, 74 cases (39.1%) of OSCC showed a mostly cytoplasmic positivity for uPAR, whereas 115 were negative. uPAR expression correlated with tumour differentiation grade and prognosis: percentage of positive cases was the greatest in G3 (70.4%) and patients positives for uPAR expression had an expectation of life lower than those for uPAR negatives.

Conclusion: The results obtained in this study suggest a role of uPAR as a potential biomarker useful to identify higher risk subgroups of OSCC patients.

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Related in: MedlinePlus

Prognostic value of uPAR expression. Kaplan-Meier curves for OSCC patients correlating uPAR expression and overall survival (A) (p = 0.0053), survival in grade 1 (B) (p = 0.0447), grade 2 (C) (p = 0.3427) and grade 3 (D) (p = 0.7143) tumours. Dotted line, uPAR negative cases ; continuous line, uPAR positive cases.
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Figure 2: Prognostic value of uPAR expression. Kaplan-Meier curves for OSCC patients correlating uPAR expression and overall survival (A) (p = 0.0053), survival in grade 1 (B) (p = 0.0447), grade 2 (C) (p = 0.3427) and grade 3 (D) (p = 0.7143) tumours. Dotted line, uPAR negative cases ; continuous line, uPAR positive cases.

Mentions: To investigate whether uPAR expression was of prognostic relevance, survival analysis was performed and overall survival was calculated using the log-rank statistics. Interestingly, Kaplan-Meier plot, comparing overall survival rates for patients with tumours displaying positive immunohistochemical staining for uPAR versus patients with uPAR negative OSCC (Fig. 2A), revealed a statistically significant association with prognosis (log-rank test, p = 0.0053). Patients with uPAR positive tumours had a lower life expectancy than those with uPAR negative tumours. Kaplan-Meier curves correlating uPAR expression with grading showed a statistical correlation between overall survival and grade 1 with a shorter overall survival for uPAR positives patients (log-rank test, p = 0.0447) (Fig. 2B), while no correlation was found with grade 2 and 3 (Fig. 2C, D).


Prognostic value analysis of urokinase-type plasminogen activator receptor in oral squamous cell carcinoma: an immunohistochemical study.

Bacchiocchi R, Rubini C, Pierpaoli E, Borghetti G, Procacci P, Nocini PF, Santarelli A, Rocchetti R, Ciavarella D, Lo Muzio L, Fazioli F - BMC Cancer (2008)

Prognostic value of uPAR expression. Kaplan-Meier curves for OSCC patients correlating uPAR expression and overall survival (A) (p = 0.0053), survival in grade 1 (B) (p = 0.0447), grade 2 (C) (p = 0.3427) and grade 3 (D) (p = 0.7143) tumours. Dotted line, uPAR negative cases ; continuous line, uPAR positive cases.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2527016&req=5

Figure 2: Prognostic value of uPAR expression. Kaplan-Meier curves for OSCC patients correlating uPAR expression and overall survival (A) (p = 0.0053), survival in grade 1 (B) (p = 0.0447), grade 2 (C) (p = 0.3427) and grade 3 (D) (p = 0.7143) tumours. Dotted line, uPAR negative cases ; continuous line, uPAR positive cases.
Mentions: To investigate whether uPAR expression was of prognostic relevance, survival analysis was performed and overall survival was calculated using the log-rank statistics. Interestingly, Kaplan-Meier plot, comparing overall survival rates for patients with tumours displaying positive immunohistochemical staining for uPAR versus patients with uPAR negative OSCC (Fig. 2A), revealed a statistically significant association with prognosis (log-rank test, p = 0.0053). Patients with uPAR positive tumours had a lower life expectancy than those with uPAR negative tumours. Kaplan-Meier curves correlating uPAR expression with grading showed a statistical correlation between overall survival and grade 1 with a shorter overall survival for uPAR positives patients (log-rank test, p = 0.0447) (Fig. 2B), while no correlation was found with grade 2 and 3 (Fig. 2C, D).

Bottom Line: Among tumour progression biomarkers, already known for their involvement in other neoplasia, a crucial role is ascribed to the urokinase-type plasminogen activator receptor (uPAR), which plays a multiple role in extracellular proteolysis, cell migration and tissue remodelling not only as a receptor for the zymogen pro-uPA but also as a component for cell adhesion and as a chemoattractant.The purpose of this study was to gain information on the expression of uPAR in OSCC and to verify whether this molecule can have a role as a prognostic/predictive marker for this neoplasia.The results obtained in this study suggest a role of uPAR as a potential biomarker useful to identify higher risk subgroups of OSCC patients.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Molecular Pathology and Innovative Therapies, Polytechnic University of the Marche Region, Ancona, Italy. bacchiocchi@univpm.it

ABSTRACT

Background: Oral squamous cell carcinoma (OSCC) represents the most common oral malignancy. Despite recent advances in therapy, up to 50% of the cases have relapse and/or metastasis. There is therefore a strong need for the identification of new biological markers able to predict the clinical behaviour of these lesions in order to improve quality of life and overall survival. Among tumour progression biomarkers, already known for their involvement in other neoplasia, a crucial role is ascribed to the urokinase-type plasminogen activator receptor (uPAR), which plays a multiple role in extracellular proteolysis, cell migration and tissue remodelling not only as a receptor for the zymogen pro-uPA but also as a component for cell adhesion and as a chemoattractant. The purpose of this study was to gain information on the expression of uPAR in OSCC and to verify whether this molecule can have a role as a prognostic/predictive marker for this neoplasia.

Methods: In a retrospective study, a cohort of 189 OSCC patients was investigated for uPAR expression and its cellular localization by immunohistochemistry. As standard controls, 8 normal oral mucosal tissues free of malignancy, obtained from patients with no evidence or history of oral cavity tumours, were similarly investigated. After grouping for uPAR expression, OSCCs were statistically analyzed for the variables age, gender, histological grading (G), tumour size, recurrence, TNM staging and overall survival rate.

Results: In our immunohistochemical study, 74 cases (39.1%) of OSCC showed a mostly cytoplasmic positivity for uPAR, whereas 115 were negative. uPAR expression correlated with tumour differentiation grade and prognosis: percentage of positive cases was the greatest in G3 (70.4%) and patients positives for uPAR expression had an expectation of life lower than those for uPAR negatives.

Conclusion: The results obtained in this study suggest a role of uPAR as a potential biomarker useful to identify higher risk subgroups of OSCC patients.

Show MeSH
Related in: MedlinePlus