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Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model.

Leichsenring A, Bäcker I, Wendt W, Andriske M, Schmitz B, Stichel CC, Lübbert H - BMC Neurosci (2008)

Bottom Line: While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology.Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T.The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology, Ruhr-University of Bochum, Bochum, Germany. anna.leichsenring@rub.de

ABSTRACT

Background: Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology. In the present study we focused on two cathepsins, CATS and CATX, and studied their spatiotemporal expression and activity during the development and progression of neuropathic pain in the CNS of the rat 5th lumbar spinal nerve transection model (L5T).

Results: Immediately after the lesion, both cathepsins, CATS and CATX, were upregulated in the spinal cord. Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T. The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain. The cellular distribution of CATS and CATX was, however, considerably different.

Conclusion: The cellular distribution and the spatio-temporal development of the altered expression of CATS and CATX suggest that these proteins are important players in the spinal mechanisms involved in chronic pain induction and maintenance.

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Related in: MedlinePlus

Phenotyping of CATS and CATX cells. Double-immunfluorescence of the spinal cord shows colocalization of CATS with the astrocyte marker GFAP (A'-A"') and colocalization of CATX with the microglial marker PT66 (B'-B"') and the macrophage marker ED1 (C'-C"'). Large motoneurons expressed CATX (D) and CATS (E). Scale bars, 10 μm (A, B), 5 μm (C), 20 μm (D, E).
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Figure 6: Phenotyping of CATS and CATX cells. Double-immunfluorescence of the spinal cord shows colocalization of CATS with the astrocyte marker GFAP (A'-A"') and colocalization of CATX with the microglial marker PT66 (B'-B"') and the macrophage marker ED1 (C'-C"'). Large motoneurons expressed CATX (D) and CATS (E). Scale bars, 10 μm (A, B), 5 μm (C), 20 μm (D, E).

Mentions: To determine the phenotype of CATS and CATX cells in vivo we performed double immunofluorescence. The majority of CATS-immunopositive cells expressed the microglia-marker PT66 or the astrocyte-marker GFAP (Fig. 6A'–A"'), while only a small number of neurons were CATS-immunopositive (Fig. 6E). In contrast, CATX-immunoreactivity colocalizes only with single glial cells (Fig. 6B'–B"') but was more abundant in neurons (Fig. 6D). All ED1-immunopositive macrophages expressed both proteins (Fig. 6C'–C"').


Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model.

Leichsenring A, Bäcker I, Wendt W, Andriske M, Schmitz B, Stichel CC, Lübbert H - BMC Neurosci (2008)

Phenotyping of CATS and CATX cells. Double-immunfluorescence of the spinal cord shows colocalization of CATS with the astrocyte marker GFAP (A'-A"') and colocalization of CATX with the microglial marker PT66 (B'-B"') and the macrophage marker ED1 (C'-C"'). Large motoneurons expressed CATX (D) and CATS (E). Scale bars, 10 μm (A, B), 5 μm (C), 20 μm (D, E).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2527007&req=5

Figure 6: Phenotyping of CATS and CATX cells. Double-immunfluorescence of the spinal cord shows colocalization of CATS with the astrocyte marker GFAP (A'-A"') and colocalization of CATX with the microglial marker PT66 (B'-B"') and the macrophage marker ED1 (C'-C"'). Large motoneurons expressed CATX (D) and CATS (E). Scale bars, 10 μm (A, B), 5 μm (C), 20 μm (D, E).
Mentions: To determine the phenotype of CATS and CATX cells in vivo we performed double immunofluorescence. The majority of CATS-immunopositive cells expressed the microglia-marker PT66 or the astrocyte-marker GFAP (Fig. 6A'–A"'), while only a small number of neurons were CATS-immunopositive (Fig. 6E). In contrast, CATX-immunoreactivity colocalizes only with single glial cells (Fig. 6B'–B"') but was more abundant in neurons (Fig. 6D). All ED1-immunopositive macrophages expressed both proteins (Fig. 6C'–C"').

Bottom Line: While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology.Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T.The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology, Ruhr-University of Bochum, Bochum, Germany. anna.leichsenring@rub.de

ABSTRACT

Background: Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology. In the present study we focused on two cathepsins, CATS and CATX, and studied their spatiotemporal expression and activity during the development and progression of neuropathic pain in the CNS of the rat 5th lumbar spinal nerve transection model (L5T).

Results: Immediately after the lesion, both cathepsins, CATS and CATX, were upregulated in the spinal cord. Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T. The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain. The cellular distribution of CATS and CATX was, however, considerably different.

Conclusion: The cellular distribution and the spatio-temporal development of the altered expression of CATS and CATX suggest that these proteins are important players in the spinal mechanisms involved in chronic pain induction and maintenance.

Show MeSH
Related in: MedlinePlus