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Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model.

Leichsenring A, Bäcker I, Wendt W, Andriske M, Schmitz B, Stichel CC, Lübbert H - BMC Neurosci (2008)

Bottom Line: While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology.Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T.The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology, Ruhr-University of Bochum, Bochum, Germany. anna.leichsenring@rub.de

ABSTRACT

Background: Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology. In the present study we focused on two cathepsins, CATS and CATX, and studied their spatiotemporal expression and activity during the development and progression of neuropathic pain in the CNS of the rat 5th lumbar spinal nerve transection model (L5T).

Results: Immediately after the lesion, both cathepsins, CATS and CATX, were upregulated in the spinal cord. Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T. The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain. The cellular distribution of CATS and CATX was, however, considerably different.

Conclusion: The cellular distribution and the spatio-temporal development of the altered expression of CATS and CATX suggest that these proteins are important players in the spinal mechanisms involved in chronic pain induction and maintenance.

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Related in: MedlinePlus

Spatiotemporal progression of CATS-/CATX-immunoreactivities in the spinal cord after L5T. Cranial progression of cathepsin upregulation during the first 5 weeks after transection. The different expression patterns in the transverse plane are symbolized by different fillings of the bars. Both cathepsins exhibited the same spatial and temporal distribution pattern up to 35 d after transection.
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Figure 3: Spatiotemporal progression of CATS-/CATX-immunoreactivities in the spinal cord after L5T. Cranial progression of cathepsin upregulation during the first 5 weeks after transection. The different expression patterns in the transverse plane are symbolized by different fillings of the bars. Both cathepsins exhibited the same spatial and temporal distribution pattern up to 35 d after transection.

Mentions: The first changes of CATS- and CATX-immunoreactivities were already notable 1 d after L5T. For both proteins we observed upregulations that were restricted to the ipsilateral fasciculus gracilis, the dorsal horn and layer IX in the ventral horn in the lumbar segment (Figs. 2 and 3). Within these regions, the number of small CATS- as well as CATX-immunopositive cells increased substantially (Fig. 2). Moreover, we found a numerical increase in CATS-immunopositive neurons, while the number of CATX-immunopositive neurons was constant. Interestingly, numerous small CATS as well as CATX-immunopositive cells engulfed large motoneurons in the ventral horn (Fig. 2G, I). Within the following days the ipsilateral increase in immunopositive cells in the fasciculus gracilis spread caudocranially to the upper SC segments and reached the gracile nucleus at 1 w after injury (Figs. 2L, N and 3). At that time-point this nucleus exhibited morphological signs of degeneration (data not shown).


Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model.

Leichsenring A, Bäcker I, Wendt W, Andriske M, Schmitz B, Stichel CC, Lübbert H - BMC Neurosci (2008)

Spatiotemporal progression of CATS-/CATX-immunoreactivities in the spinal cord after L5T. Cranial progression of cathepsin upregulation during the first 5 weeks after transection. The different expression patterns in the transverse plane are symbolized by different fillings of the bars. Both cathepsins exhibited the same spatial and temporal distribution pattern up to 35 d after transection.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2527007&req=5

Figure 3: Spatiotemporal progression of CATS-/CATX-immunoreactivities in the spinal cord after L5T. Cranial progression of cathepsin upregulation during the first 5 weeks after transection. The different expression patterns in the transverse plane are symbolized by different fillings of the bars. Both cathepsins exhibited the same spatial and temporal distribution pattern up to 35 d after transection.
Mentions: The first changes of CATS- and CATX-immunoreactivities were already notable 1 d after L5T. For both proteins we observed upregulations that were restricted to the ipsilateral fasciculus gracilis, the dorsal horn and layer IX in the ventral horn in the lumbar segment (Figs. 2 and 3). Within these regions, the number of small CATS- as well as CATX-immunopositive cells increased substantially (Fig. 2). Moreover, we found a numerical increase in CATS-immunopositive neurons, while the number of CATX-immunopositive neurons was constant. Interestingly, numerous small CATS as well as CATX-immunopositive cells engulfed large motoneurons in the ventral horn (Fig. 2G, I). Within the following days the ipsilateral increase in immunopositive cells in the fasciculus gracilis spread caudocranially to the upper SC segments and reached the gracile nucleus at 1 w after injury (Figs. 2L, N and 3). At that time-point this nucleus exhibited morphological signs of degeneration (data not shown).

Bottom Line: While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology.Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T.The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology, Ruhr-University of Bochum, Bochum, Germany. anna.leichsenring@rub.de

ABSTRACT

Background: Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology. In the present study we focused on two cathepsins, CATS and CATX, and studied their spatiotemporal expression and activity during the development and progression of neuropathic pain in the CNS of the rat 5th lumbar spinal nerve transection model (L5T).

Results: Immediately after the lesion, both cathepsins, CATS and CATX, were upregulated in the spinal cord. Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T. The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain. The cellular distribution of CATS and CATX was, however, considerably different.

Conclusion: The cellular distribution and the spatio-temporal development of the altered expression of CATS and CATX suggest that these proteins are important players in the spinal mechanisms involved in chronic pain induction and maintenance.

Show MeSH
Related in: MedlinePlus