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Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model.

Leichsenring A, Bäcker I, Wendt W, Andriske M, Schmitz B, Stichel CC, Lübbert H - BMC Neurosci (2008)

Bottom Line: While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology.Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T.The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology, Ruhr-University of Bochum, Bochum, Germany. anna.leichsenring@rub.de

ABSTRACT

Background: Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology. In the present study we focused on two cathepsins, CATS and CATX, and studied their spatiotemporal expression and activity during the development and progression of neuropathic pain in the CNS of the rat 5th lumbar spinal nerve transection model (L5T).

Results: Immediately after the lesion, both cathepsins, CATS and CATX, were upregulated in the spinal cord. Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T. The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain. The cellular distribution of CATS and CATX was, however, considerably different.

Conclusion: The cellular distribution and the spatio-temporal development of the altered expression of CATS and CATX suggest that these proteins are important players in the spinal mechanisms involved in chronic pain induction and maintenance.

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Related in: MedlinePlus

CATS- and CATX-immunohistochemistry in normal rat spinal cord. Representative examples of CATS- (A, C, E-G) and CATX-immunostained (B, D, H-J) sections of the L5 segment. CATS-immunopositive deposits are localized in small glial-like cells (C, E, F) that distributed homogenously throughout the section (A), while CATX is mostly found in large neurons (D, H) and only few small cells are intensely stained (D, J). G, I: Sections incubated with preabsorbed primary antibodies are free of immunostaining. Scale bars, 500 μm (A, B), 50 μm (C, D), 20 μm (E-J).
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Figure 1: CATS- and CATX-immunohistochemistry in normal rat spinal cord. Representative examples of CATS- (A, C, E-G) and CATX-immunostained (B, D, H-J) sections of the L5 segment. CATS-immunopositive deposits are localized in small glial-like cells (C, E, F) that distributed homogenously throughout the section (A), while CATX is mostly found in large neurons (D, H) and only few small cells are intensely stained (D, J). G, I: Sections incubated with preabsorbed primary antibodies are free of immunostaining. Scale bars, 500 μm (A, B), 50 μm (C, D), 20 μm (E-J).

Mentions: In unlesioned adult rats CATS- and CATX-immunoreactivities were found in cells of both grey and white matters (Fig. 1) throughout the entire length of the spinal cord. Most CATS-immunopositive cells were of small size and distributed uniformely (Fig. 1A, C, E, F). While CATS-immunopositive neurons were rare, CATX-immunoreactivity was found in nearly all neurons (Fig. 1B, D, H) and only in few small cells (Fig. 1D, J). The immunoreactivities were associated with spherical granules within the cytoplasm of cells, sparing the nucleus (Fig. 1E, D, H).


Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model.

Leichsenring A, Bäcker I, Wendt W, Andriske M, Schmitz B, Stichel CC, Lübbert H - BMC Neurosci (2008)

CATS- and CATX-immunohistochemistry in normal rat spinal cord. Representative examples of CATS- (A, C, E-G) and CATX-immunostained (B, D, H-J) sections of the L5 segment. CATS-immunopositive deposits are localized in small glial-like cells (C, E, F) that distributed homogenously throughout the section (A), while CATX is mostly found in large neurons (D, H) and only few small cells are intensely stained (D, J). G, I: Sections incubated with preabsorbed primary antibodies are free of immunostaining. Scale bars, 500 μm (A, B), 50 μm (C, D), 20 μm (E-J).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2527007&req=5

Figure 1: CATS- and CATX-immunohistochemistry in normal rat spinal cord. Representative examples of CATS- (A, C, E-G) and CATX-immunostained (B, D, H-J) sections of the L5 segment. CATS-immunopositive deposits are localized in small glial-like cells (C, E, F) that distributed homogenously throughout the section (A), while CATX is mostly found in large neurons (D, H) and only few small cells are intensely stained (D, J). G, I: Sections incubated with preabsorbed primary antibodies are free of immunostaining. Scale bars, 500 μm (A, B), 50 μm (C, D), 20 μm (E-J).
Mentions: In unlesioned adult rats CATS- and CATX-immunoreactivities were found in cells of both grey and white matters (Fig. 1) throughout the entire length of the spinal cord. Most CATS-immunopositive cells were of small size and distributed uniformely (Fig. 1A, C, E, F). While CATS-immunopositive neurons were rare, CATX-immunoreactivity was found in nearly all neurons (Fig. 1B, D, H) and only in few small cells (Fig. 1D, J). The immunoreactivities were associated with spherical granules within the cytoplasm of cells, sparing the nucleus (Fig. 1E, D, H).

Bottom Line: While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology.Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T.The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Physiology, Ruhr-University of Bochum, Bochum, Germany. anna.leichsenring@rub.de

ABSTRACT

Background: Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology. In the present study we focused on two cathepsins, CATS and CATX, and studied their spatiotemporal expression and activity during the development and progression of neuropathic pain in the CNS of the rat 5th lumbar spinal nerve transection model (L5T).

Results: Immediately after the lesion, both cathepsins, CATS and CATX, were upregulated in the spinal cord. Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T. The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain. The cellular distribution of CATS and CATX was, however, considerably different.

Conclusion: The cellular distribution and the spatio-temporal development of the altered expression of CATS and CATX suggest that these proteins are important players in the spinal mechanisms involved in chronic pain induction and maintenance.

Show MeSH
Related in: MedlinePlus