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Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.

Kruse R, Essén-Gustavsson B, Fossum C, Jensen-Waern M - Acta Vet. Scand. (2008)

Bottom Line: TNF-alpha increased in all animals after inoculation, with a peak during dysentery, and IL-6 was found in 3 animals during dysentery and in the 2 animals that did not develop clinical signs of disease.IL-10 was found in all sick animals during the recovery period.IFN-gamma was not detected on any occasion.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Swedish University of Agricultural Sciences, P.O. Box 7054, S-750 07, Uppsala, Sweden. robert.kruse@kv.slu.se

ABSTRACT

Background: Knowledge of the cytokine response at infection with Brachyspira hyodysenteriae can help understanding disease mechanism involved during swine dysentery. Since this knowledge is still limited the aim of the present study was to induce dysentery experimentally in pigs and to monitor the development of important immunoregulatory cytokines in blood collected at various stages of the disease.

Methods: Ten conventional pigs (~23 kg) were orally inoculated with Brachyspira hyodysenteriae B204T. Eight animals developed muco-haemorrhagic diarrhoea with impaired general body condition. Blood was sampled before inoculation and repeatedly during acute dysentery and recovery periods and cytokine levels of IL-1beta, IL-6, Il-10, TNF-alpha and IFN-gamma were measured by ELISA.

Results: IL-1beta was increased at the beginning of the dysentery period and coincided with the appearance of Serum amyloid A and clinical signs of disease. TNF-alpha increased in all animals after inoculation, with a peak during dysentery, and IL-6 was found in 3 animals during dysentery and in the 2 animals that did not develop clinical signs of disease. IL-10 was found in all sick animals during the recovery period. IFN-gamma was not detected on any occasion.

Conclusion: B. hyodysenteriae inoculation induced production of systemic levels of IL-1beta during the dysentery period and increased levels of IL-10 coincided with recovery from dysentery.

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Related in: MedlinePlus

Serum concentrations of cytokines during experimentally induced swine dysentery. Serum concentrations of IL-1β, TNF-α and IL-10 before inoculation, during clinical signs of dysentery, and during the recovery period are shown by the box plot. The shaded circles illustrates the individual values of the two animals that remained clinically healthy sampled before inoculation and at days 4, 14, 21, 28 and 35 post-inoculation. The shaded areas above zero represent the detection limit of the assays. * significantly different from the pre-inoculation value.
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Figure 1: Serum concentrations of cytokines during experimentally induced swine dysentery. Serum concentrations of IL-1β, TNF-α and IL-10 before inoculation, during clinical signs of dysentery, and during the recovery period are shown by the box plot. The shaded circles illustrates the individual values of the two animals that remained clinically healthy sampled before inoculation and at days 4, 14, 21, 28 and 35 post-inoculation. The shaded areas above zero represent the detection limit of the assays. * significantly different from the pre-inoculation value.

Mentions: Data are presented in the text as mean ± SD. The boundaries of the box plot in figure 1 indicate the 25th percentile, the median value and the 75th percentile, whereas the whiskers indicate the 95th and 5th percentiles. One pig had to be euthanised on the second day of clinical signs because of the severity of the disease and is therefore missing from later sampling points, leaving a total of 7 dysentery-affected animals. In addition, there are three missing samples from different pigs during recovery and therefore the means at day 7 are from 5 out of 7 animals and at day 11 they are from 6 out of 7 animals. To compare differences between measurement times, analysis of variance (ANOVA, Holm-Sidak Method) for repeated measures was performed with SigmaStat software (SPSS Science, Chicago, USA). The data were regarded as significantly different at p < 0.05.


Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.

Kruse R, Essén-Gustavsson B, Fossum C, Jensen-Waern M - Acta Vet. Scand. (2008)

Serum concentrations of cytokines during experimentally induced swine dysentery. Serum concentrations of IL-1β, TNF-α and IL-10 before inoculation, during clinical signs of dysentery, and during the recovery period are shown by the box plot. The shaded circles illustrates the individual values of the two animals that remained clinically healthy sampled before inoculation and at days 4, 14, 21, 28 and 35 post-inoculation. The shaded areas above zero represent the detection limit of the assays. * significantly different from the pre-inoculation value.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2527004&req=5

Figure 1: Serum concentrations of cytokines during experimentally induced swine dysentery. Serum concentrations of IL-1β, TNF-α and IL-10 before inoculation, during clinical signs of dysentery, and during the recovery period are shown by the box plot. The shaded circles illustrates the individual values of the two animals that remained clinically healthy sampled before inoculation and at days 4, 14, 21, 28 and 35 post-inoculation. The shaded areas above zero represent the detection limit of the assays. * significantly different from the pre-inoculation value.
Mentions: Data are presented in the text as mean ± SD. The boundaries of the box plot in figure 1 indicate the 25th percentile, the median value and the 75th percentile, whereas the whiskers indicate the 95th and 5th percentiles. One pig had to be euthanised on the second day of clinical signs because of the severity of the disease and is therefore missing from later sampling points, leaving a total of 7 dysentery-affected animals. In addition, there are three missing samples from different pigs during recovery and therefore the means at day 7 are from 5 out of 7 animals and at day 11 they are from 6 out of 7 animals. To compare differences between measurement times, analysis of variance (ANOVA, Holm-Sidak Method) for repeated measures was performed with SigmaStat software (SPSS Science, Chicago, USA). The data were regarded as significantly different at p < 0.05.

Bottom Line: TNF-alpha increased in all animals after inoculation, with a peak during dysentery, and IL-6 was found in 3 animals during dysentery and in the 2 animals that did not develop clinical signs of disease.IL-10 was found in all sick animals during the recovery period.IFN-gamma was not detected on any occasion.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Clinical Sciences, Section for Comparative Physiology and Medicine, Swedish University of Agricultural Sciences, P.O. Box 7054, S-750 07, Uppsala, Sweden. robert.kruse@kv.slu.se

ABSTRACT

Background: Knowledge of the cytokine response at infection with Brachyspira hyodysenteriae can help understanding disease mechanism involved during swine dysentery. Since this knowledge is still limited the aim of the present study was to induce dysentery experimentally in pigs and to monitor the development of important immunoregulatory cytokines in blood collected at various stages of the disease.

Methods: Ten conventional pigs (~23 kg) were orally inoculated with Brachyspira hyodysenteriae B204T. Eight animals developed muco-haemorrhagic diarrhoea with impaired general body condition. Blood was sampled before inoculation and repeatedly during acute dysentery and recovery periods and cytokine levels of IL-1beta, IL-6, Il-10, TNF-alpha and IFN-gamma were measured by ELISA.

Results: IL-1beta was increased at the beginning of the dysentery period and coincided with the appearance of Serum amyloid A and clinical signs of disease. TNF-alpha increased in all animals after inoculation, with a peak during dysentery, and IL-6 was found in 3 animals during dysentery and in the 2 animals that did not develop clinical signs of disease. IL-10 was found in all sick animals during the recovery period. IFN-gamma was not detected on any occasion.

Conclusion: B. hyodysenteriae inoculation induced production of systemic levels of IL-1beta during the dysentery period and increased levels of IL-10 coincided with recovery from dysentery.

Show MeSH
Related in: MedlinePlus