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Jaceosidin induces apoptosis in human ovary cancer cells through mitochondrial pathway.

Lv W, Sheng X, Chen T, Xu Q, Xie X - J. Biomed. Biotechnol. (2008)

Bottom Line: By flow cytometric analysis, we found that Jaceosidin treatment resulted in an increased apoptosis in CAOV-3 cells.Jaceosidin also increased the level of cleaved caspase-9 and induced the cleavage of caspase-3 and poly (ADP-ribose) polymerase (PARP), while caspase-3 inhibitor Z-DEVD-FMK significantly reversed the proapoptotic effect of Jaceosidin in CAOV-3 cells.These findings suggest that the anticancer effect of Jaceosidin may be contributed by an induction of apoptosis involving cytochrome c release from mitochondria to cytosol.

View Article: PubMed Central - PubMed

Affiliation: The Second Affiliated Hospital, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.

ABSTRACT
We examined the antiproliferation effect of Jaceosidin (4', 5, 7-trihydroxy-3', 6-dimethoxyflavone) isolated from the herb of Artemisia vestita Wall on several human cancer cell lines. Jaceosidin significantly reduced the proliferation of CAOV-3, SKOV-3, HeLa, and PC3 cells in a concentration-dependent manner. A time-dependent inhibition was also observed in CAOV-3 cells by Jaceosidin. By flow cytometric analysis, we found that Jaceosidin treatment resulted in an increased apoptosis in CAOV-3 cells. The cells treated with Jaceosidin exhibited a decreased mitochondrial membrane potential. Jaceosidin also increased the level of cleaved caspase-9 and induced the cleavage of caspase-3 and poly (ADP-ribose) polymerase (PARP), while caspase-3 inhibitor Z-DEVD-FMK significantly reversed the proapoptotic effect of Jaceosidin in CAOV-3 cells. Moreover, Jaceosidin elevated the level of cytochrome c in cytosol. These findings suggest that the anticancer effect of Jaceosidin may be contributed by an induction of apoptosis involving cytochrome c release from mitochondria to cytosol.

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Related in: MedlinePlus

Jaceosidin activates caspase-3and its related proteins to promote apoptosis. Whole cell lysates prepared from CAOV-3cells, which had been incubated with jaceosidin or quercetin for 24 hours. Theproteins were subjected to Western blot analysis using the respectiveantibodies as indicated. Data are representative of three separate experiments.
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fig4: Jaceosidin activates caspase-3and its related proteins to promote apoptosis. Whole cell lysates prepared from CAOV-3cells, which had been incubated with jaceosidin or quercetin for 24 hours. Theproteins were subjected to Western blot analysis using the respectiveantibodies as indicated. Data are representative of three separate experiments.

Mentions: To examine the status of the caspase-3protein, we performed Western blot analysis by using an anticaspase-3 antibody,which recognizes both procaspase-3 and cleaved caspase-3, the p17 large/activesubunit of caspase-3. As shown in Figure 4, the p17 cleavage product appearedin lysates of CAOV-3 cells treated with 80 μM jaceosidin and 10 μM quercetin. Jaceosidinand quercetin also cleaved PARP to a band of 85 kDa. The amount of cleavedcaspase-9 was also increased significantly in the cells treated with quercetin,and slightly with jaceosidin.


Jaceosidin induces apoptosis in human ovary cancer cells through mitochondrial pathway.

Lv W, Sheng X, Chen T, Xu Q, Xie X - J. Biomed. Biotechnol. (2008)

Jaceosidin activates caspase-3and its related proteins to promote apoptosis. Whole cell lysates prepared from CAOV-3cells, which had been incubated with jaceosidin or quercetin for 24 hours. Theproteins were subjected to Western blot analysis using the respectiveantibodies as indicated. Data are representative of three separate experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2519793&req=5

fig4: Jaceosidin activates caspase-3and its related proteins to promote apoptosis. Whole cell lysates prepared from CAOV-3cells, which had been incubated with jaceosidin or quercetin for 24 hours. Theproteins were subjected to Western blot analysis using the respectiveantibodies as indicated. Data are representative of three separate experiments.
Mentions: To examine the status of the caspase-3protein, we performed Western blot analysis by using an anticaspase-3 antibody,which recognizes both procaspase-3 and cleaved caspase-3, the p17 large/activesubunit of caspase-3. As shown in Figure 4, the p17 cleavage product appearedin lysates of CAOV-3 cells treated with 80 μM jaceosidin and 10 μM quercetin. Jaceosidinand quercetin also cleaved PARP to a band of 85 kDa. The amount of cleavedcaspase-9 was also increased significantly in the cells treated with quercetin,and slightly with jaceosidin.

Bottom Line: By flow cytometric analysis, we found that Jaceosidin treatment resulted in an increased apoptosis in CAOV-3 cells.Jaceosidin also increased the level of cleaved caspase-9 and induced the cleavage of caspase-3 and poly (ADP-ribose) polymerase (PARP), while caspase-3 inhibitor Z-DEVD-FMK significantly reversed the proapoptotic effect of Jaceosidin in CAOV-3 cells.These findings suggest that the anticancer effect of Jaceosidin may be contributed by an induction of apoptosis involving cytochrome c release from mitochondria to cytosol.

View Article: PubMed Central - PubMed

Affiliation: The Second Affiliated Hospital, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.

ABSTRACT
We examined the antiproliferation effect of Jaceosidin (4', 5, 7-trihydroxy-3', 6-dimethoxyflavone) isolated from the herb of Artemisia vestita Wall on several human cancer cell lines. Jaceosidin significantly reduced the proliferation of CAOV-3, SKOV-3, HeLa, and PC3 cells in a concentration-dependent manner. A time-dependent inhibition was also observed in CAOV-3 cells by Jaceosidin. By flow cytometric analysis, we found that Jaceosidin treatment resulted in an increased apoptosis in CAOV-3 cells. The cells treated with Jaceosidin exhibited a decreased mitochondrial membrane potential. Jaceosidin also increased the level of cleaved caspase-9 and induced the cleavage of caspase-3 and poly (ADP-ribose) polymerase (PARP), while caspase-3 inhibitor Z-DEVD-FMK significantly reversed the proapoptotic effect of Jaceosidin in CAOV-3 cells. Moreover, Jaceosidin elevated the level of cytochrome c in cytosol. These findings suggest that the anticancer effect of Jaceosidin may be contributed by an induction of apoptosis involving cytochrome c release from mitochondria to cytosol.

Show MeSH
Related in: MedlinePlus