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First detection, isolation and molecular characterization of infectious salmon anaemia virus associated with clinical disease in farmed Atlantic salmon (Salmo salar) in Chile.

Godoy MG, Aedo A, Kibenge MJ, Groman DB, Yason CV, Grothusen H, Lisperguer A, Calbucura M, Avendaño F, Imilán M, Jarpa M, Kibenge FS - BMC Vet. Res. (2008)

Bottom Line: In conclusion, the present work constitutes the first report of a case of ISA in farmed Atlantic salmon in Chile.The clinical signs and lesions are consistent with the classical descriptions of the disease in marine-farmed Atlantic salmon in the Northern hemisphere.The outbreak was caused by ISAV of European genotype (or Genotype I) of HPR 7b but distinct from common European Genotype ISAV isolates.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biovac SA, Puerto Montt, Chile. mgodoy@biovac.cl

ABSTRACT

Background: Infectious salmon anaemia (ISA) is a viral disease of marine-farmed Atlantic salmon (Salmo salar) caused by ISA virus (ISAV), which belongs to the genus Isavirus, family Orthomyxoviridae. The virus is considered to be carried by marine wild fish and for over 25 years has caused major disease outbreaks in marine-farmed Atlantic salmon in the Northern hemisphere. In the Southern hemisphere, ISAV was first detected in Chile in 1999 in marine-farmed Coho salmon (Oncorhynchus kisutch). In contrast to the classical presentation of ISA in Atlantic salmon, the presence of ISAV in Chile until now has only been associated with a clinical condition called Icterus Syndrome in Coho salmon and virus isolation has not always been possible. During the winter of 2007, unexplained mortalities were registered in market-size Atlantic salmon in a grow-out site located in Chiloé in Region X of Chile. We report here the diagnostic findings of the first significant clinical outbreak of ISA in marine-farmed Atlantic salmon in Chile and the first characterization of the ISAV isolated from the affected fish.

Results: In mid-June 2007, an Atlantic salmon marine farm site located in central Chiloé Island in Region X of Chile registered a sudden increase in mortality following recovery from an outbreak of Pisciricketsiosis, which rose to a cumulative mortality of 13.6% by harvest time. Based on the clinical signs and lesions in the affected fish, and laboratory tests performed on the fish tissues, a confirmatory diagnosis of ISA was made; the first time ISA in its classical presentation and for the first time affecting farmed Atlantic salmon in Chile. Rapid sequencing of the virus-specific RT-PCR products amplified from the fish tissues identified the virus to belong to the European genotype (Genotype I) of the highly polymorphic region (HPR) group HPR 7b, but with an 11-amino acid insert in the fusion glycoprotein, and ability to cause cytopathic effects (CPE) in CHSE-214 cell line, characteristics which make it distinct from common European Genotype ISAV isolates from Europe and North America.

Conclusion: In conclusion, the present work constitutes the first report of a case of ISA in farmed Atlantic salmon in Chile. The clinical signs and lesions are consistent with the classical descriptions of the disease in marine-farmed Atlantic salmon in the Northern hemisphere. The outbreak was caused by ISAV of European genotype (or Genotype I) of HPR 7b but distinct from common European Genotype ISAV isolates.

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Alignment of amino acid sequences in the highly polymorphic region (HPR) of the HE genes of various strains of ISAV (modified from Kibenge et al. [19]). The amino acid sequence corresponding to the HE-HPR of the Chilean ISAV in this disease outbreak is highlighted in yellow, and identifies it as HPR7. Sequences that are not determined are indicated by dots, and amino acid (aa) deletions in the HPR are indicated by dashes. The HPR groups identified are as reported by Nylund et al. [23] and Plarre et al. [24]; other sources of information are indicated as *Markussen et al. [20], **Cunningham et al. [25], ***Cook-Versloot et al. [26], and ****Mjaaland et al. [27].
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Figure 6: Alignment of amino acid sequences in the highly polymorphic region (HPR) of the HE genes of various strains of ISAV (modified from Kibenge et al. [19]). The amino acid sequence corresponding to the HE-HPR of the Chilean ISAV in this disease outbreak is highlighted in yellow, and identifies it as HPR7. Sequences that are not determined are indicated by dots, and amino acid (aa) deletions in the HPR are indicated by dashes. The HPR groups identified are as reported by Nylund et al. [23] and Plarre et al. [24]; other sources of information are indicated as *Markussen et al. [20], **Cunningham et al. [25], ***Cook-Versloot et al. [26], and ****Mjaaland et al. [27].

Mentions: Alignment of the amino acid sequences of RNA segment 5, which encodes the F glycoprotein, revealed that the new Chile ISAV has a small insert of 11 amino acids (or 33 nucleotides) relative to ISAV strains from Europe and North America near the putative proteolytic cleavage site of the precursor F0 protein (Fig. 5). This insert has 100% sequence identity with RNA segment 2 of Genotype I. ISAV RNA segment 2 encodes the PB1 polymerase [22]. Alignment of the amino acid sequences of RNA segment 6, which encodes the HE glycoprotein, revealed that the new Chile ISAV belongs to HPR7b of the European genotype as described by Nylund et al. [23] and Plarre et al. [24] (Fig. 6) and reviewed by Rimstad et al. [18].


First detection, isolation and molecular characterization of infectious salmon anaemia virus associated with clinical disease in farmed Atlantic salmon (Salmo salar) in Chile.

Godoy MG, Aedo A, Kibenge MJ, Groman DB, Yason CV, Grothusen H, Lisperguer A, Calbucura M, Avendaño F, Imilán M, Jarpa M, Kibenge FS - BMC Vet. Res. (2008)

Alignment of amino acid sequences in the highly polymorphic region (HPR) of the HE genes of various strains of ISAV (modified from Kibenge et al. [19]). The amino acid sequence corresponding to the HE-HPR of the Chilean ISAV in this disease outbreak is highlighted in yellow, and identifies it as HPR7. Sequences that are not determined are indicated by dots, and amino acid (aa) deletions in the HPR are indicated by dashes. The HPR groups identified are as reported by Nylund et al. [23] and Plarre et al. [24]; other sources of information are indicated as *Markussen et al. [20], **Cunningham et al. [25], ***Cook-Versloot et al. [26], and ****Mjaaland et al. [27].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2519066&req=5

Figure 6: Alignment of amino acid sequences in the highly polymorphic region (HPR) of the HE genes of various strains of ISAV (modified from Kibenge et al. [19]). The amino acid sequence corresponding to the HE-HPR of the Chilean ISAV in this disease outbreak is highlighted in yellow, and identifies it as HPR7. Sequences that are not determined are indicated by dots, and amino acid (aa) deletions in the HPR are indicated by dashes. The HPR groups identified are as reported by Nylund et al. [23] and Plarre et al. [24]; other sources of information are indicated as *Markussen et al. [20], **Cunningham et al. [25], ***Cook-Versloot et al. [26], and ****Mjaaland et al. [27].
Mentions: Alignment of the amino acid sequences of RNA segment 5, which encodes the F glycoprotein, revealed that the new Chile ISAV has a small insert of 11 amino acids (or 33 nucleotides) relative to ISAV strains from Europe and North America near the putative proteolytic cleavage site of the precursor F0 protein (Fig. 5). This insert has 100% sequence identity with RNA segment 2 of Genotype I. ISAV RNA segment 2 encodes the PB1 polymerase [22]. Alignment of the amino acid sequences of RNA segment 6, which encodes the HE glycoprotein, revealed that the new Chile ISAV belongs to HPR7b of the European genotype as described by Nylund et al. [23] and Plarre et al. [24] (Fig. 6) and reviewed by Rimstad et al. [18].

Bottom Line: In conclusion, the present work constitutes the first report of a case of ISA in farmed Atlantic salmon in Chile.The clinical signs and lesions are consistent with the classical descriptions of the disease in marine-farmed Atlantic salmon in the Northern hemisphere.The outbreak was caused by ISAV of European genotype (or Genotype I) of HPR 7b but distinct from common European Genotype ISAV isolates.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biovac SA, Puerto Montt, Chile. mgodoy@biovac.cl

ABSTRACT

Background: Infectious salmon anaemia (ISA) is a viral disease of marine-farmed Atlantic salmon (Salmo salar) caused by ISA virus (ISAV), which belongs to the genus Isavirus, family Orthomyxoviridae. The virus is considered to be carried by marine wild fish and for over 25 years has caused major disease outbreaks in marine-farmed Atlantic salmon in the Northern hemisphere. In the Southern hemisphere, ISAV was first detected in Chile in 1999 in marine-farmed Coho salmon (Oncorhynchus kisutch). In contrast to the classical presentation of ISA in Atlantic salmon, the presence of ISAV in Chile until now has only been associated with a clinical condition called Icterus Syndrome in Coho salmon and virus isolation has not always been possible. During the winter of 2007, unexplained mortalities were registered in market-size Atlantic salmon in a grow-out site located in Chiloé in Region X of Chile. We report here the diagnostic findings of the first significant clinical outbreak of ISA in marine-farmed Atlantic salmon in Chile and the first characterization of the ISAV isolated from the affected fish.

Results: In mid-June 2007, an Atlantic salmon marine farm site located in central Chiloé Island in Region X of Chile registered a sudden increase in mortality following recovery from an outbreak of Pisciricketsiosis, which rose to a cumulative mortality of 13.6% by harvest time. Based on the clinical signs and lesions in the affected fish, and laboratory tests performed on the fish tissues, a confirmatory diagnosis of ISA was made; the first time ISA in its classical presentation and for the first time affecting farmed Atlantic salmon in Chile. Rapid sequencing of the virus-specific RT-PCR products amplified from the fish tissues identified the virus to belong to the European genotype (Genotype I) of the highly polymorphic region (HPR) group HPR 7b, but with an 11-amino acid insert in the fusion glycoprotein, and ability to cause cytopathic effects (CPE) in CHSE-214 cell line, characteristics which make it distinct from common European Genotype ISAV isolates from Europe and North America.

Conclusion: In conclusion, the present work constitutes the first report of a case of ISA in farmed Atlantic salmon in Chile. The clinical signs and lesions are consistent with the classical descriptions of the disease in marine-farmed Atlantic salmon in the Northern hemisphere. The outbreak was caused by ISAV of European genotype (or Genotype I) of HPR 7b but distinct from common European Genotype ISAV isolates.

Show MeSH
Related in: MedlinePlus