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Anti-inflammatory effects of ciprofloxacin in S. aureus Newman induced nasal inflammation in vitro.

Sachse F, von Eiff C, Becker K, Rudack C - J Inflamm (Lond) (2008)

Bottom Line: Similarly, addition of clarithromycin was associated with a reduction of IL-8 synthesis although this effect was not significant.Coincubation with prednisolone resulted in a significant reduction of IL-8 levels.Inhibitory effects were comparable to those of prednisolone and clarithromycin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Otorhinolaryngology Head and Neck Surgery, University of Muenster, Germany. sachsef@mednet.uni-muenster.de.

ABSTRACT

Objectives: Chronic rhinosinusitis (CRS) is a chronic inflammatory disease of the nasal mucosa. Recent studies suggest that S. aureus enterotoxins may play an etiologic role in the development of CRS. Apart from surgery and repeated courses of steroids, macrolide antibiotics have been reported to exert anti-inflammatory effects in CRS. Similar effects have been reported for fluoroquinolones on various cell types. Since these effects have poorly been characterized in CRS, we examined anti-inflammatory effects of ciprofloxacin on human nasal epithelial cells (HNECs).

Methods: Inflammation was induced in HNECs cultured from nasal turbinate mucosa with supernatants of S. aureus Newman for 12 hours. Subsequently, HNECs were coincubated with S. aureus Newman and ciprofloxacin (1.5 x 10-5 M), clarithromycin (10-6 M) or prednisolone (10-5 M) for another 12 hours. IL-8 synthesis was quantified after 12 and 24 hours by ELISA.

Results: Stimulation with S. aureus Newman supernatants was associated with an increase of IL-8 synthesis after 12 hours in all experiments. During the second 12 hours, IL-8 synthesis decreased and this effect was independent from any stimulus or inhibitor. However, coincubation of HNECs with ciprofloxacin was associated with a more extensive decrease of IL-8 synthesis. Similarly, addition of clarithromycin was associated with a reduction of IL-8 synthesis although this effect was not significant. Coincubation with prednisolone resulted in a significant reduction of IL-8 levels.

Conclusion: Ciprofloxacin exerts anti-inflammatory effects in S. aureus Newman driven nasal inflammation. Inhibitory effects were comparable to those of prednisolone and clarithromycin.

No MeSH data available.


Related in: MedlinePlus

IL-8 synthesis (pg/ml) following coincubation of HNECs with S. aureus supernatants and prednisolone (10-5 M). Bars represent means ± standard deviation of three independent experiments. Addition or absence of S. aureus Newman supernatants and prednisolone is indicated by "+" and "-". p < 0.05 (*) was considered significant.
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Figure 3: IL-8 synthesis (pg/ml) following coincubation of HNECs with S. aureus supernatants and prednisolone (10-5 M). Bars represent means ± standard deviation of three independent experiments. Addition or absence of S. aureus Newman supernatants and prednisolone is indicated by "+" and "-". p < 0.05 (*) was considered significant.

Mentions: Addition of S. aureus Newman supernatants was associated with an increase of IL-8 synthesis after 12 hours in all experiments. However, we observed a significant decrease of IL-8 synthesis in controls and in controls of antibiotics and prednisolone that occurred during the second 12 hours (12–24 hours). Moreover, stimulation with S. aureus Newman alone was associated with an identical effect: the maximum of IL-8 synthesis was determined after 12 hours, whereas further stimulation with S. aureus Newman demonstrated decrease of IL-8 synthesis during the second 12 hours (12–24 hours). Taken together these results suggest that a time-dependent decrease of IL-8 synthesis occurred in HNECs which was independent from any stimulus or inhibitor. As a consequence, the time-dependent decrease of IL-8 synthesis had to be considered when analyzing inhibitory effects of ciprofloxacin, clarithromycin and prednisolone (fig. 1, 2, 3).


Anti-inflammatory effects of ciprofloxacin in S. aureus Newman induced nasal inflammation in vitro.

Sachse F, von Eiff C, Becker K, Rudack C - J Inflamm (Lond) (2008)

IL-8 synthesis (pg/ml) following coincubation of HNECs with S. aureus supernatants and prednisolone (10-5 M). Bars represent means ± standard deviation of three independent experiments. Addition or absence of S. aureus Newman supernatants and prednisolone is indicated by "+" and "-". p < 0.05 (*) was considered significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2507710&req=5

Figure 3: IL-8 synthesis (pg/ml) following coincubation of HNECs with S. aureus supernatants and prednisolone (10-5 M). Bars represent means ± standard deviation of three independent experiments. Addition or absence of S. aureus Newman supernatants and prednisolone is indicated by "+" and "-". p < 0.05 (*) was considered significant.
Mentions: Addition of S. aureus Newman supernatants was associated with an increase of IL-8 synthesis after 12 hours in all experiments. However, we observed a significant decrease of IL-8 synthesis in controls and in controls of antibiotics and prednisolone that occurred during the second 12 hours (12–24 hours). Moreover, stimulation with S. aureus Newman alone was associated with an identical effect: the maximum of IL-8 synthesis was determined after 12 hours, whereas further stimulation with S. aureus Newman demonstrated decrease of IL-8 synthesis during the second 12 hours (12–24 hours). Taken together these results suggest that a time-dependent decrease of IL-8 synthesis occurred in HNECs which was independent from any stimulus or inhibitor. As a consequence, the time-dependent decrease of IL-8 synthesis had to be considered when analyzing inhibitory effects of ciprofloxacin, clarithromycin and prednisolone (fig. 1, 2, 3).

Bottom Line: Similarly, addition of clarithromycin was associated with a reduction of IL-8 synthesis although this effect was not significant.Coincubation with prednisolone resulted in a significant reduction of IL-8 levels.Inhibitory effects were comparable to those of prednisolone and clarithromycin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Otorhinolaryngology Head and Neck Surgery, University of Muenster, Germany. sachsef@mednet.uni-muenster.de.

ABSTRACT

Objectives: Chronic rhinosinusitis (CRS) is a chronic inflammatory disease of the nasal mucosa. Recent studies suggest that S. aureus enterotoxins may play an etiologic role in the development of CRS. Apart from surgery and repeated courses of steroids, macrolide antibiotics have been reported to exert anti-inflammatory effects in CRS. Similar effects have been reported for fluoroquinolones on various cell types. Since these effects have poorly been characterized in CRS, we examined anti-inflammatory effects of ciprofloxacin on human nasal epithelial cells (HNECs).

Methods: Inflammation was induced in HNECs cultured from nasal turbinate mucosa with supernatants of S. aureus Newman for 12 hours. Subsequently, HNECs were coincubated with S. aureus Newman and ciprofloxacin (1.5 x 10-5 M), clarithromycin (10-6 M) or prednisolone (10-5 M) for another 12 hours. IL-8 synthesis was quantified after 12 and 24 hours by ELISA.

Results: Stimulation with S. aureus Newman supernatants was associated with an increase of IL-8 synthesis after 12 hours in all experiments. During the second 12 hours, IL-8 synthesis decreased and this effect was independent from any stimulus or inhibitor. However, coincubation of HNECs with ciprofloxacin was associated with a more extensive decrease of IL-8 synthesis. Similarly, addition of clarithromycin was associated with a reduction of IL-8 synthesis although this effect was not significant. Coincubation with prednisolone resulted in a significant reduction of IL-8 levels.

Conclusion: Ciprofloxacin exerts anti-inflammatory effects in S. aureus Newman driven nasal inflammation. Inhibitory effects were comparable to those of prednisolone and clarithromycin.

No MeSH data available.


Related in: MedlinePlus