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Regionally and climatically restricted patterns of distribution of genetic diversity in a migratory bat species, Miniopterus schreibersii (Chiroptera: Vespertilionidae).

Bilgin R, Karataş A, Coraman E, Disotell T, Morales JC - BMC Evol. Biol. (2008)

Bottom Line: Our results showed differentiation in mitochondrial DNA coupled with weaker nuclear differentiation.We found evidence for restriction of lineages to geographical areas for hundreds of generations.The results showed that the most likely ancestral haplotype was restricted to the same geographic area (the Balkans) for at least 6,000 years.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Environmental Sciences, Boğaziçi University, Bebek 34342, Istanbul, Turkey. rasit.bilgin@boun.edu.tr

ABSTRACT

Background: Various mechanisms such as geographic barriers and glacial episodes have been proposed as determinants of intra-specific and inter-specific differentiation of populations, and the distribution of their genetic diversity. More recently, habitat and climate differences, and corresponding adaptations have been shown to be forces influencing the phylogeographic evolution of some vertebrates. In this study, we examined the contribution of these various factors on the genetic differentiation of the bent-winged bat, Miniopterus schreibersii, in southeastern Europe and Anatolia.

Results and conclusion: Our results showed differentiation in mitochondrial DNA coupled with weaker nuclear differentiation. We found evidence for restriction of lineages to geographical areas for hundreds of generations. The results showed that the most likely ancestral haplotype was restricted to the same geographic area (the Balkans) for at least 6,000 years. We were able to delineate the migration routes during the population expansion process, which followed the coasts and the inland for different nested mitochondrial clades. Hence, we were able to describe a scenario showing how multiple biotic and abiotic events including glacial periods, climate and historical dispersal patterns complemented each other in causing regional and local differentiation within a species.

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a) Neighbor joining phylogenetic tree of mtDNA sequences for M. schreibersii. Bootstrap support values for clades S and P are underlined for the neighbor joining phylogram and in italics for the maximum parsimony tree. A sample of M. schreibersii from Indonesia was used as outgroup. b) Statistical parsimony network of haplotypes (S1–S40) for clade S. The unlabeled circles indicate hypothetical haplotypes. The size of each circle is proportional to the frequency of the particular haplotype in the sample. c) The statistical parsimony network of haplotypes (P1–P10) for clade P. The unlabeled circles indicate hypothetical haplotypes. The size of each circle is proportional to the frequency of the particular haplotype in the sample.
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Figure 2: a) Neighbor joining phylogenetic tree of mtDNA sequences for M. schreibersii. Bootstrap support values for clades S and P are underlined for the neighbor joining phylogram and in italics for the maximum parsimony tree. A sample of M. schreibersii from Indonesia was used as outgroup. b) Statistical parsimony network of haplotypes (S1–S40) for clade S. The unlabeled circles indicate hypothetical haplotypes. The size of each circle is proportional to the frequency of the particular haplotype in the sample. c) The statistical parsimony network of haplotypes (P1–P10) for clade P. The unlabeled circles indicate hypothetical haplotypes. The size of each circle is proportional to the frequency of the particular haplotype in the sample.

Mentions: Phylogenetic trees for M. schreibersii were constructed using neighbor joining and parsimony methods (Figure 2a). A sample of M. schreibersii from Indonesia was included in the analysis as an outgroup. In the neighbor joining tree, two monophyletic clades have been identified, clade S and clade P, with high bootstrap support (83% and 100%, respectively). A heuristic maximum parsimony search also gave high bootstrap support to the nodes delimiting clades S (98%) and P (81%). In this analysis, the transversions were weighted against transitions based on their frequency (1:7). The C.I and R.I were both 0.908 for this tree.


Regionally and climatically restricted patterns of distribution of genetic diversity in a migratory bat species, Miniopterus schreibersii (Chiroptera: Vespertilionidae).

Bilgin R, Karataş A, Coraman E, Disotell T, Morales JC - BMC Evol. Biol. (2008)

a) Neighbor joining phylogenetic tree of mtDNA sequences for M. schreibersii. Bootstrap support values for clades S and P are underlined for the neighbor joining phylogram and in italics for the maximum parsimony tree. A sample of M. schreibersii from Indonesia was used as outgroup. b) Statistical parsimony network of haplotypes (S1–S40) for clade S. The unlabeled circles indicate hypothetical haplotypes. The size of each circle is proportional to the frequency of the particular haplotype in the sample. c) The statistical parsimony network of haplotypes (P1–P10) for clade P. The unlabeled circles indicate hypothetical haplotypes. The size of each circle is proportional to the frequency of the particular haplotype in the sample.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2483726&req=5

Figure 2: a) Neighbor joining phylogenetic tree of mtDNA sequences for M. schreibersii. Bootstrap support values for clades S and P are underlined for the neighbor joining phylogram and in italics for the maximum parsimony tree. A sample of M. schreibersii from Indonesia was used as outgroup. b) Statistical parsimony network of haplotypes (S1–S40) for clade S. The unlabeled circles indicate hypothetical haplotypes. The size of each circle is proportional to the frequency of the particular haplotype in the sample. c) The statistical parsimony network of haplotypes (P1–P10) for clade P. The unlabeled circles indicate hypothetical haplotypes. The size of each circle is proportional to the frequency of the particular haplotype in the sample.
Mentions: Phylogenetic trees for M. schreibersii were constructed using neighbor joining and parsimony methods (Figure 2a). A sample of M. schreibersii from Indonesia was included in the analysis as an outgroup. In the neighbor joining tree, two monophyletic clades have been identified, clade S and clade P, with high bootstrap support (83% and 100%, respectively). A heuristic maximum parsimony search also gave high bootstrap support to the nodes delimiting clades S (98%) and P (81%). In this analysis, the transversions were weighted against transitions based on their frequency (1:7). The C.I and R.I were both 0.908 for this tree.

Bottom Line: Our results showed differentiation in mitochondrial DNA coupled with weaker nuclear differentiation.We found evidence for restriction of lineages to geographical areas for hundreds of generations.The results showed that the most likely ancestral haplotype was restricted to the same geographic area (the Balkans) for at least 6,000 years.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Environmental Sciences, Boğaziçi University, Bebek 34342, Istanbul, Turkey. rasit.bilgin@boun.edu.tr

ABSTRACT

Background: Various mechanisms such as geographic barriers and glacial episodes have been proposed as determinants of intra-specific and inter-specific differentiation of populations, and the distribution of their genetic diversity. More recently, habitat and climate differences, and corresponding adaptations have been shown to be forces influencing the phylogeographic evolution of some vertebrates. In this study, we examined the contribution of these various factors on the genetic differentiation of the bent-winged bat, Miniopterus schreibersii, in southeastern Europe and Anatolia.

Results and conclusion: Our results showed differentiation in mitochondrial DNA coupled with weaker nuclear differentiation. We found evidence for restriction of lineages to geographical areas for hundreds of generations. The results showed that the most likely ancestral haplotype was restricted to the same geographic area (the Balkans) for at least 6,000 years. We were able to delineate the migration routes during the population expansion process, which followed the coasts and the inland for different nested mitochondrial clades. Hence, we were able to describe a scenario showing how multiple biotic and abiotic events including glacial periods, climate and historical dispersal patterns complemented each other in causing regional and local differentiation within a species.

Show MeSH