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Molecular characterisation of Mycobacterium bovis isolated from cattle slaughtered at the Bamako abattoir in Mali.

Müller B, Steiner B, Bonfoh B, Fané A, Smith NH, Zinsstag J - BMC Vet. Res. (2008)

Bottom Line: Spoligotype patterns from thirteen of the strains lacked spacer 30, a characteristic common in strains of M. bovis found in Chad, Cameroon and Nigeria.The additional absence of spacer 6 in the majority of these strains suggests a Mali specific clone.The spoligotype patterns of the remaining strains suggest that they may have been of European origin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Swiss Tropical Institute, Basel, Switzerland. borna.mueller@unibas.ch

ABSTRACT

Background: Mali is one of the most important livestock producers of the Sahel region of Africa. A high frequency of bovine tuberculosis (BTB) has been reported but surveillance and control schemes are restricted to abattoir inspections only. The objective of this study was to conduct, for the first time, molecular characterisation of Mycobacterium bovis strains isolated from cattle slaughtered at the Bamako abattoir. Of 3330 animals screened only 60 exhibited gross visible lesions. From these animals, twenty strains of M. bovis were isolated and characterised by spoligotyping.

Results: Organ lesions typical of BTB were most often detected in the liver, followed by the lung and the peritoneum. M. bovis was isolated from 20 animals and 7 different spoligotypes were observed among these 20 strains; three of the patterns had not been previously reported. Spoligotype patterns from thirteen of the strains lacked spacer 30, a characteristic common in strains of M. bovis found in Chad, Cameroon and Nigeria. However, unlike the other three Central African countries, the majority of spoligotype patterns observed in Mali also lacked spacer 6. Of the remaining seven strains, six had spoligotype patterns identical to strains commonly isolated in France and Spain.

Conclusion: Two groups of M. bovis were detected in cattle slaughtered at the Bamako abattoir. The spoligotype pattern of the first group has similarities to strains previously observed in Chad, Cameroon and Nigeria. The additional absence of spacer 6 in the majority of these strains suggests a Mali specific clone. The spoligotype patterns of the remaining strains suggest that they may have been of European origin.

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Spoligotypes and VNTR typing patterns of M. bovis strains isolated from slaughter cattle at Bamako abattoir in Mali. Spacers 39–43 were absent from all spoligotype patterns. SB numbers were taken from . VNTR typing targeted loci ETR A – F [24].
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Figure 1: Spoligotypes and VNTR typing patterns of M. bovis strains isolated from slaughter cattle at Bamako abattoir in Mali. Spacers 39–43 were absent from all spoligotype patterns. SB numbers were taken from . VNTR typing targeted loci ETR A – F [24].

Mentions: Infection with M. bovis was highly associated with the presence of lung lesions (N = 44, chi-squared = 23.7, p < 0.001); in 79% of the animals exhibiting lung lesions, M. bovis infection could be confirmed. The association was less strong for liver lesions (N = 48, chi-squared = 3.9, p < 0.05); only 41% of the animals with liver lesions were shown to be infected with M. bovis. However, in all 9 cases where M. bovis infection was detected in animals with liver lesions, lung lesions were present as well. No association was found between infection with M. bovis and lesions in organs other than the liver and lungs. Strains of M. bovis isolated in different organs of the same animal showed the same spoligotype pattern. Altogether among the 20 strains of M. bovis isolated, seven different spoligotypes were observed; four had been previously reported (SB0944, SB0300, SB0134 and SB0944) and the remaining three were designated SB1410, SB1411 and SB1412 by (figure 1).


Molecular characterisation of Mycobacterium bovis isolated from cattle slaughtered at the Bamako abattoir in Mali.

Müller B, Steiner B, Bonfoh B, Fané A, Smith NH, Zinsstag J - BMC Vet. Res. (2008)

Spoligotypes and VNTR typing patterns of M. bovis strains isolated from slaughter cattle at Bamako abattoir in Mali. Spacers 39–43 were absent from all spoligotype patterns. SB numbers were taken from . VNTR typing targeted loci ETR A – F [24].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2483712&req=5

Figure 1: Spoligotypes and VNTR typing patterns of M. bovis strains isolated from slaughter cattle at Bamako abattoir in Mali. Spacers 39–43 were absent from all spoligotype patterns. SB numbers were taken from . VNTR typing targeted loci ETR A – F [24].
Mentions: Infection with M. bovis was highly associated with the presence of lung lesions (N = 44, chi-squared = 23.7, p < 0.001); in 79% of the animals exhibiting lung lesions, M. bovis infection could be confirmed. The association was less strong for liver lesions (N = 48, chi-squared = 3.9, p < 0.05); only 41% of the animals with liver lesions were shown to be infected with M. bovis. However, in all 9 cases where M. bovis infection was detected in animals with liver lesions, lung lesions were present as well. No association was found between infection with M. bovis and lesions in organs other than the liver and lungs. Strains of M. bovis isolated in different organs of the same animal showed the same spoligotype pattern. Altogether among the 20 strains of M. bovis isolated, seven different spoligotypes were observed; four had been previously reported (SB0944, SB0300, SB0134 and SB0944) and the remaining three were designated SB1410, SB1411 and SB1412 by (figure 1).

Bottom Line: Spoligotype patterns from thirteen of the strains lacked spacer 30, a characteristic common in strains of M. bovis found in Chad, Cameroon and Nigeria.The additional absence of spacer 6 in the majority of these strains suggests a Mali specific clone.The spoligotype patterns of the remaining strains suggest that they may have been of European origin.

View Article: PubMed Central - HTML - PubMed

Affiliation: Swiss Tropical Institute, Basel, Switzerland. borna.mueller@unibas.ch

ABSTRACT

Background: Mali is one of the most important livestock producers of the Sahel region of Africa. A high frequency of bovine tuberculosis (BTB) has been reported but surveillance and control schemes are restricted to abattoir inspections only. The objective of this study was to conduct, for the first time, molecular characterisation of Mycobacterium bovis strains isolated from cattle slaughtered at the Bamako abattoir. Of 3330 animals screened only 60 exhibited gross visible lesions. From these animals, twenty strains of M. bovis were isolated and characterised by spoligotyping.

Results: Organ lesions typical of BTB were most often detected in the liver, followed by the lung and the peritoneum. M. bovis was isolated from 20 animals and 7 different spoligotypes were observed among these 20 strains; three of the patterns had not been previously reported. Spoligotype patterns from thirteen of the strains lacked spacer 30, a characteristic common in strains of M. bovis found in Chad, Cameroon and Nigeria. However, unlike the other three Central African countries, the majority of spoligotype patterns observed in Mali also lacked spacer 6. Of the remaining seven strains, six had spoligotype patterns identical to strains commonly isolated in France and Spain.

Conclusion: Two groups of M. bovis were detected in cattle slaughtered at the Bamako abattoir. The spoligotype pattern of the first group has similarities to strains previously observed in Chad, Cameroon and Nigeria. The additional absence of spacer 6 in the majority of these strains suggests a Mali specific clone. The spoligotype patterns of the remaining strains suggest that they may have been of European origin.

Show MeSH
Related in: MedlinePlus