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NEW SPIN ON SPINDLE SIZE

View Article: PubMed Central

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When a cell builds a spindle, microtubules extend from several locations, including the chromosomes and the centrosomes... By preventing TPX2 from activating Aurora A, Bird and Hyman tested whether this pair helps determine spindle length in human cells... Instead, the researchers incorporated the mutant gene into a bacterial artificial chromosome that also harbored the gene's normal regulatory sequences... Inserted into a cell, this combination produces a more realistic pattern of gene activity, the scientists say... When the scientists blocked Aurora A activation in this way, a squat spindle resulted... Although the cells could divide, mitosis often went awry, and they sometimes split into three daughter cells... With TPX2 unable to switch on Aurora A, microtubules that sprouted early in mitosis were unstable, the researchers found... Moreover, although microtubules extended from the centrosomes without Aurora A, they didn't grow from chromosomes... That result raises the possibility that Aurora A determines spindle size by spurring elongation of microtubules from the chromosomes... The researchers now want to determine what proteins Aurora A turns on to exert its effect.

No MeSH data available.


A normal spindle (left) contrasts with frizzy ones from cells lacking TPX2 (right).
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fig1: A normal spindle (left) contrasts with frizzy ones from cells lacking TPX2 (right).


NEW SPIN ON SPINDLE SIZE
A normal spindle (left) contrasts with frizzy ones from cells lacking TPX2 (right).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2483529&req=5

fig1: A normal spindle (left) contrasts with frizzy ones from cells lacking TPX2 (right).

View Article: PubMed Central

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

When a cell builds a spindle, microtubules extend from several locations, including the chromosomes and the centrosomes... By preventing TPX2 from activating Aurora A, Bird and Hyman tested whether this pair helps determine spindle length in human cells... Instead, the researchers incorporated the mutant gene into a bacterial artificial chromosome that also harbored the gene's normal regulatory sequences... Inserted into a cell, this combination produces a more realistic pattern of gene activity, the scientists say... When the scientists blocked Aurora A activation in this way, a squat spindle resulted... Although the cells could divide, mitosis often went awry, and they sometimes split into three daughter cells... With TPX2 unable to switch on Aurora A, microtubules that sprouted early in mitosis were unstable, the researchers found... Moreover, although microtubules extended from the centrosomes without Aurora A, they didn't grow from chromosomes... That result raises the possibility that Aurora A determines spindle size by spurring elongation of microtubules from the chromosomes... The researchers now want to determine what proteins Aurora A turns on to exert its effect.

No MeSH data available.