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Operator-assisted harvesting of protein crystals using a universal micromanipulation robot.

Viola R, Carman P, Walsh J, Miller E, Benning M, Frankel D, McPherson A, Cudney B, Rupp B - J Appl Crystallogr (2007)

Bottom Line: High-throughput crystallography has reached a level of automation where complete computer-assisted robotic crystallization pipelines are capable of cocktail preparation, crystallization plate setup, and inspection and interpretation of results.To address the final frontier in achieving fully automated high-throughput crystallography, the prototype of an anthropomorphic six-axis universal micromanipulation robot (UMR) has been designed and tested; this UMR is capable of operator-assisted harvesting and cryoquenching of protein crystals as small as 10 microm from a variety of 96-well plates.The UMR is equipped with a versatile tool exchanger providing full operational flexibility.

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ABSTRACT
High-throughput crystallography has reached a level of automation where complete computer-assisted robotic crystallization pipelines are capable of cocktail preparation, crystallization plate setup, and inspection and interpretation of results. While mounting of crystal pins, data collection and structure solution are highly automated, crystal harvesting and cryocooling remain formidable challenges towards full automation. To address the final frontier in achieving fully automated high-throughput crystallography, the prototype of an anthropomorphic six-axis universal micromanipulation robot (UMR) has been designed and tested; this UMR is capable of operator-assisted harvesting and cryoquenching of protein crystals as small as 10 microm from a variety of 96-well plates. The UMR is equipped with a versatile tool exchanger providing full operational flexibility. Trypsin crystals harvested and cryoquenched using the UMR have yielded a 1.5 A structure demonstrating the feasibility of robotic protein crystal harvesting.

No MeSH data available.


Related in: MedlinePlus

Detailed view of the tool exchanger and tool storage (left panel), and the plate manipulator tool attached to the tool exchanger (right panel).
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fig2: Detailed view of the tool exchanger and tool storage (left panel), and the plate manipulator tool attached to the tool exchanger (right panel).

Mentions: Special end effectors were designed to provide for plate handling, tape cutting and crystal harvesting (Fig. 2 ▶). In each instance, these designs simply adapted existing manual process tools and provided a means for precisely aligning these tools relative to the robot. Each end effector incorporated kinematic mounting features allowing it to be accurately registered in a storage library at the edge of the work cell. Testing confirmed the system’s ability to reliably perform all basic material handling tasks.


Operator-assisted harvesting of protein crystals using a universal micromanipulation robot.

Viola R, Carman P, Walsh J, Miller E, Benning M, Frankel D, McPherson A, Cudney B, Rupp B - J Appl Crystallogr (2007)

Detailed view of the tool exchanger and tool storage (left panel), and the plate manipulator tool attached to the tool exchanger (right panel).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2483483&req=5

fig2: Detailed view of the tool exchanger and tool storage (left panel), and the plate manipulator tool attached to the tool exchanger (right panel).
Mentions: Special end effectors were designed to provide for plate handling, tape cutting and crystal harvesting (Fig. 2 ▶). In each instance, these designs simply adapted existing manual process tools and provided a means for precisely aligning these tools relative to the robot. Each end effector incorporated kinematic mounting features allowing it to be accurately registered in a storage library at the edge of the work cell. Testing confirmed the system’s ability to reliably perform all basic material handling tasks.

Bottom Line: High-throughput crystallography has reached a level of automation where complete computer-assisted robotic crystallization pipelines are capable of cocktail preparation, crystallization plate setup, and inspection and interpretation of results.To address the final frontier in achieving fully automated high-throughput crystallography, the prototype of an anthropomorphic six-axis universal micromanipulation robot (UMR) has been designed and tested; this UMR is capable of operator-assisted harvesting and cryoquenching of protein crystals as small as 10 microm from a variety of 96-well plates.The UMR is equipped with a versatile tool exchanger providing full operational flexibility.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
High-throughput crystallography has reached a level of automation where complete computer-assisted robotic crystallization pipelines are capable of cocktail preparation, crystallization plate setup, and inspection and interpretation of results. While mounting of crystal pins, data collection and structure solution are highly automated, crystal harvesting and cryocooling remain formidable challenges towards full automation. To address the final frontier in achieving fully automated high-throughput crystallography, the prototype of an anthropomorphic six-axis universal micromanipulation robot (UMR) has been designed and tested; this UMR is capable of operator-assisted harvesting and cryoquenching of protein crystals as small as 10 microm from a variety of 96-well plates. The UMR is equipped with a versatile tool exchanger providing full operational flexibility. Trypsin crystals harvested and cryoquenched using the UMR have yielded a 1.5 A structure demonstrating the feasibility of robotic protein crystal harvesting.

No MeSH data available.


Related in: MedlinePlus