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Expression of novel extracellular sulfatases Sulf-1 and Sulf-2 in normal and osteoarthritic articular cartilage.

Otsuki S, Taniguchi N, Grogan SP, D'Lima D, Kinoshita M, Lotz M - Arthritis Res. Ther. (2008)

Bottom Line: OA cartilage showed significantly higher Sulf-1 and Sulf-2 mRNA expression as compared with normal human articular cartilage.The results show low levels of Sulf expression, restricted to the superficial zone in normal articular cartilage.This increased Sulf expression may change the sulfation patterns of heparan sulfate proteoglycans and growth factor activities and thus contribute to abnormal chondrocyte activation and cartilage degradation in OA.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Arthritis Research, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. otsuki@scripps.edu

ABSTRACT

Introduction: Changes in sulfation of cartilage glycosaminoglycans as mediated by sulfatases can regulate growth factor signaling. The aim of this study was to analyze expression patterns of recently identified extracellular sulfatases Sulf-1 and Sulf-2 in articular cartilage and chondrocytes.

Methods: Sulf-1 and Sulf-2 expressions in human articular cartilage from normal donors and patients with osteoarthritis (OA) and in normal and aged mouse joints were analyzed by real-time polymerase chain reaction, immunohistochemistry, and Western blotting.

Results: In normal articular cartilage, Sulf-1 and Sulf-2 mRNAs and proteins were expressed predominantly in the superficial zone. OA cartilage showed significantly higher Sulf-1 and Sulf-2 mRNA expression as compared with normal human articular cartilage. Sulf protein expression in OA cartilage was prominent in the cell clusters. Western blotting revealed a profound increase in Sulf protein levels in human OA cartilage. In normal mouse joints, Sulf expression was similar to human cartilage, and with increasing age, there was a marked upregulation of Sulf.

Conclusion: The results show low levels of Sulf expression, restricted to the superficial zone in normal articular cartilage. Sulf mRNA and protein levels are increased in aging and OA cartilage. This increased Sulf expression may change the sulfation patterns of heparan sulfate proteoglycans and growth factor activities and thus contribute to abnormal chondrocyte activation and cartilage degradation in OA.

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Sulf-1 and Sulf-2 protein expression in normal and osteoarthritis (OA) cartilage. Sulf protein expression in normal (43 years old; Mankin score: 2) and OA (79 years old; Mankin score: 9) cartilage. Immunoblottings of Sulf-1, Sulf-2, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) were performed on protein extracts from normal and OA cartilage.
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Figure 5: Sulf-1 and Sulf-2 protein expression in normal and osteoarthritis (OA) cartilage. Sulf protein expression in normal (43 years old; Mankin score: 2) and OA (79 years old; Mankin score: 9) cartilage. Immunoblottings of Sulf-1, Sulf-2, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) were performed on protein extracts from normal and OA cartilage.

Mentions: Western blotting was performed to visualize Sulf proteins and determine differences in the expression between normal and OA. In total protein extracts from normal cartilage, Sulf-1 and Sulf-2 were not detectable. In contrast, high levels of Sulf-1 and Sulf-2 protein were detected in OA cartilage (Figure 5). The major Sulf-1 and Sulf-2 protein bands migrated at approximately 72 kDa, which is the molecular mass of the secreted proteins [17,30,31].


Expression of novel extracellular sulfatases Sulf-1 and Sulf-2 in normal and osteoarthritic articular cartilage.

Otsuki S, Taniguchi N, Grogan SP, D'Lima D, Kinoshita M, Lotz M - Arthritis Res. Ther. (2008)

Sulf-1 and Sulf-2 protein expression in normal and osteoarthritis (OA) cartilage. Sulf protein expression in normal (43 years old; Mankin score: 2) and OA (79 years old; Mankin score: 9) cartilage. Immunoblottings of Sulf-1, Sulf-2, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) were performed on protein extracts from normal and OA cartilage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2483452&req=5

Figure 5: Sulf-1 and Sulf-2 protein expression in normal and osteoarthritis (OA) cartilage. Sulf protein expression in normal (43 years old; Mankin score: 2) and OA (79 years old; Mankin score: 9) cartilage. Immunoblottings of Sulf-1, Sulf-2, and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) were performed on protein extracts from normal and OA cartilage.
Mentions: Western blotting was performed to visualize Sulf proteins and determine differences in the expression between normal and OA. In total protein extracts from normal cartilage, Sulf-1 and Sulf-2 were not detectable. In contrast, high levels of Sulf-1 and Sulf-2 protein were detected in OA cartilage (Figure 5). The major Sulf-1 and Sulf-2 protein bands migrated at approximately 72 kDa, which is the molecular mass of the secreted proteins [17,30,31].

Bottom Line: OA cartilage showed significantly higher Sulf-1 and Sulf-2 mRNA expression as compared with normal human articular cartilage.The results show low levels of Sulf expression, restricted to the superficial zone in normal articular cartilage.This increased Sulf expression may change the sulfation patterns of heparan sulfate proteoglycans and growth factor activities and thus contribute to abnormal chondrocyte activation and cartilage degradation in OA.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Arthritis Research, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. otsuki@scripps.edu

ABSTRACT

Introduction: Changes in sulfation of cartilage glycosaminoglycans as mediated by sulfatases can regulate growth factor signaling. The aim of this study was to analyze expression patterns of recently identified extracellular sulfatases Sulf-1 and Sulf-2 in articular cartilage and chondrocytes.

Methods: Sulf-1 and Sulf-2 expressions in human articular cartilage from normal donors and patients with osteoarthritis (OA) and in normal and aged mouse joints were analyzed by real-time polymerase chain reaction, immunohistochemistry, and Western blotting.

Results: In normal articular cartilage, Sulf-1 and Sulf-2 mRNAs and proteins were expressed predominantly in the superficial zone. OA cartilage showed significantly higher Sulf-1 and Sulf-2 mRNA expression as compared with normal human articular cartilage. Sulf protein expression in OA cartilage was prominent in the cell clusters. Western blotting revealed a profound increase in Sulf protein levels in human OA cartilage. In normal mouse joints, Sulf expression was similar to human cartilage, and with increasing age, there was a marked upregulation of Sulf.

Conclusion: The results show low levels of Sulf expression, restricted to the superficial zone in normal articular cartilage. Sulf mRNA and protein levels are increased in aging and OA cartilage. This increased Sulf expression may change the sulfation patterns of heparan sulfate proteoglycans and growth factor activities and thus contribute to abnormal chondrocyte activation and cartilage degradation in OA.

Show MeSH
Related in: MedlinePlus