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Presence of Helicobacter pylori in a Mexican Pre-Columbian Mummy.

Castillo-Rojas G, Cerbón MA, López-Vidal Y - BMC Microbiol. (2008)

Bottom Line: These PCR products were hybridized with a pHp probe.Nucleotide sequence analysis showed homology with H. pylori in 98 of 99% when compared with the gene bank nucleotide sequence.Only one sample of gastric remnant H. pylori-positive with 16S rRNA gene was also positive for ureB gene from H. pylori.

View Article: PubMed Central - HTML - PubMed

Affiliation: Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico. gcastillo55@hotmail.com

ABSTRACT

Background: Recent studies showed that Helicobacter pylori existed in the New World prior to the arrival of Columbus. The purpose of the present study was to detect the presence of Helicobacter pylori in pre-Columbian mummies from Northern Mexico.

Methods: Six samples were studied (four samples of gastric remains, tongue-soft palate, and brain remained as negative controls) from two of the six naturally mummified corpses studied (adult male and infant male). Samples were taken from tissues suitable for DNA amplification by Polymerase chain reaction (PCR). DNA was extracted and H. pylori detection was carried out by PCR and hybridized with the pHp probe from 16S rRNA gene. The purified PCR products were cloned and sequenced in both directions. DNA sequences were analyzed with ALIGN and BLAST software. A second amplification was performed using ureB gene by real-time PCR.

Results: From four samples of gastric remnant, only two were H. pylori-positive for amplification of a 109 bp DNA fragment; the remaining two were negative, as were the tongue-soft palate and the brain biopsies as well. These PCR products were hybridized with a pHp probe. Nucleotide sequence analysis showed homology with H. pylori in 98 of 99% when compared with the gene bank nucleotide sequence. Only one sample of gastric remnant H. pylori-positive with 16S rRNA gene was also positive for ureB gene from H. pylori.

Conclusion: This data supported infection with H. pylori in Mexican pre-Columbian mummies dating from approximately 1,350 AC.

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Related in: MedlinePlus

Polymerase chain reaction (PCR) amplification and hybridization of biopsy samples. A) PCR products of the 109-bp fragment of 16S rRNA obtained from H. pylori. Line: 1) gastric remains I; 2) gastric remains II; 3) tongue-soft palate; 4) brain; 5) H. pylori ATCC 43504; 6) H. pylori 84–183, and 7) negative control reaction. M = Molecular weight marker (100-bp DNA ladder). B) Hybridization of PCR products with pHp probe.
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Figure 2: Polymerase chain reaction (PCR) amplification and hybridization of biopsy samples. A) PCR products of the 109-bp fragment of 16S rRNA obtained from H. pylori. Line: 1) gastric remains I; 2) gastric remains II; 3) tongue-soft palate; 4) brain; 5) H. pylori ATCC 43504; 6) H. pylori 84–183, and 7) negative control reaction. M = Molecular weight marker (100-bp DNA ladder). B) Hybridization of PCR products with pHp probe.

Mentions: Of four biopsies of gastric remains, only two were H. pylori-positive for amplification of a 109-bp DNA fragment (from mummy 2); the remaining two gastric remains-tissue samples were H. pylori- negative, as were the tongue-soft palate and brain-tissue samples (Figure 2A). PCR products were H. pylori-positive to hybridization with H. pylori probe (pHp) (Figure 2B), confirming that the amplified product of 109-bp is H. pylori-specific. The results of sample amplification and hybridization performed at the second laboratory was in agreement with our results: only gastric-remains samples from mummy 2 were positive, while the other remaining samples of gastric remains, in addition to tongue-soft palate and brain, were also negative.


Presence of Helicobacter pylori in a Mexican Pre-Columbian Mummy.

Castillo-Rojas G, Cerbón MA, López-Vidal Y - BMC Microbiol. (2008)

Polymerase chain reaction (PCR) amplification and hybridization of biopsy samples. A) PCR products of the 109-bp fragment of 16S rRNA obtained from H. pylori. Line: 1) gastric remains I; 2) gastric remains II; 3) tongue-soft palate; 4) brain; 5) H. pylori ATCC 43504; 6) H. pylori 84–183, and 7) negative control reaction. M = Molecular weight marker (100-bp DNA ladder). B) Hybridization of PCR products with pHp probe.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2483284&req=5

Figure 2: Polymerase chain reaction (PCR) amplification and hybridization of biopsy samples. A) PCR products of the 109-bp fragment of 16S rRNA obtained from H. pylori. Line: 1) gastric remains I; 2) gastric remains II; 3) tongue-soft palate; 4) brain; 5) H. pylori ATCC 43504; 6) H. pylori 84–183, and 7) negative control reaction. M = Molecular weight marker (100-bp DNA ladder). B) Hybridization of PCR products with pHp probe.
Mentions: Of four biopsies of gastric remains, only two were H. pylori-positive for amplification of a 109-bp DNA fragment (from mummy 2); the remaining two gastric remains-tissue samples were H. pylori- negative, as were the tongue-soft palate and brain-tissue samples (Figure 2A). PCR products were H. pylori-positive to hybridization with H. pylori probe (pHp) (Figure 2B), confirming that the amplified product of 109-bp is H. pylori-specific. The results of sample amplification and hybridization performed at the second laboratory was in agreement with our results: only gastric-remains samples from mummy 2 were positive, while the other remaining samples of gastric remains, in addition to tongue-soft palate and brain, were also negative.

Bottom Line: These PCR products were hybridized with a pHp probe.Nucleotide sequence analysis showed homology with H. pylori in 98 of 99% when compared with the gene bank nucleotide sequence.Only one sample of gastric remnant H. pylori-positive with 16S rRNA gene was also positive for ureB gene from H. pylori.

View Article: PubMed Central - HTML - PubMed

Affiliation: Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico. gcastillo55@hotmail.com

ABSTRACT

Background: Recent studies showed that Helicobacter pylori existed in the New World prior to the arrival of Columbus. The purpose of the present study was to detect the presence of Helicobacter pylori in pre-Columbian mummies from Northern Mexico.

Methods: Six samples were studied (four samples of gastric remains, tongue-soft palate, and brain remained as negative controls) from two of the six naturally mummified corpses studied (adult male and infant male). Samples were taken from tissues suitable for DNA amplification by Polymerase chain reaction (PCR). DNA was extracted and H. pylori detection was carried out by PCR and hybridized with the pHp probe from 16S rRNA gene. The purified PCR products were cloned and sequenced in both directions. DNA sequences were analyzed with ALIGN and BLAST software. A second amplification was performed using ureB gene by real-time PCR.

Results: From four samples of gastric remnant, only two were H. pylori-positive for amplification of a 109 bp DNA fragment; the remaining two were negative, as were the tongue-soft palate and the brain biopsies as well. These PCR products were hybridized with a pHp probe. Nucleotide sequence analysis showed homology with H. pylori in 98 of 99% when compared with the gene bank nucleotide sequence. Only one sample of gastric remnant H. pylori-positive with 16S rRNA gene was also positive for ureB gene from H. pylori.

Conclusion: This data supported infection with H. pylori in Mexican pre-Columbian mummies dating from approximately 1,350 AC.

Show MeSH
Related in: MedlinePlus