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Presence of Helicobacter pylori in a Mexican Pre-Columbian Mummy.

Castillo-Rojas G, Cerbón MA, López-Vidal Y - BMC Microbiol. (2008)

Bottom Line: These PCR products were hybridized with a pHp probe.Nucleotide sequence analysis showed homology with H. pylori in 98 of 99% when compared with the gene bank nucleotide sequence.Only one sample of gastric remnant H. pylori-positive with 16S rRNA gene was also positive for ureB gene from H. pylori.

View Article: PubMed Central - HTML - PubMed

Affiliation: Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico. gcastillo55@hotmail.com

ABSTRACT

Background: Recent studies showed that Helicobacter pylori existed in the New World prior to the arrival of Columbus. The purpose of the present study was to detect the presence of Helicobacter pylori in pre-Columbian mummies from Northern Mexico.

Methods: Six samples were studied (four samples of gastric remains, tongue-soft palate, and brain remained as negative controls) from two of the six naturally mummified corpses studied (adult male and infant male). Samples were taken from tissues suitable for DNA amplification by Polymerase chain reaction (PCR). DNA was extracted and H. pylori detection was carried out by PCR and hybridized with the pHp probe from 16S rRNA gene. The purified PCR products were cloned and sequenced in both directions. DNA sequences were analyzed with ALIGN and BLAST software. A second amplification was performed using ureB gene by real-time PCR.

Results: From four samples of gastric remnant, only two were H. pylori-positive for amplification of a 109 bp DNA fragment; the remaining two were negative, as were the tongue-soft palate and the brain biopsies as well. These PCR products were hybridized with a pHp probe. Nucleotide sequence analysis showed homology with H. pylori in 98 of 99% when compared with the gene bank nucleotide sequence. Only one sample of gastric remnant H. pylori-positive with 16S rRNA gene was also positive for ureB gene from H. pylori.

Conclusion: This data supported infection with H. pylori in Mexican pre-Columbian mummies dating from approximately 1,350 AC.

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Mexican Pre-Columbian mummies. Yellow box showed the mummy that was Helicobacter pylori-positive.
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Figure 1: Mexican Pre-Columbian mummies. Yellow box showed the mummy that was Helicobacter pylori-positive.

Mentions: Four pre-Columbian mummified bodies recovered in the funeral cave of La Ventana, located in the Chihuahua State desert and two additional mummies recovered in a cave located in the state of Durango (Figure 1) were studied. Climatic conditions in the caves allowed natural preservation of the bodies throughout centuries. (We are grateful to Dr. Josefina Mansilla at Instituto de Antropología e Historia de México who allowed us to obtain mummy samples). The samples were studied to obtain valuable information on the soft tissues and for internal-organ visualization using a Pentax EG-1840 gastroscope, a Pentax VB-1530 videobronchoscope, and a Pentax ELC-2800 videolaparoscope. (We kindly appreciate the support from Dr. Fernando Mundo of the Hospital Angeles del Pedregal in Mexico City for sample collection). Sampling was carried out in a room with no history of work with H. pylori at the Instituto de Antropología e Historia de México. Tissue specimens from the mummies were acquired by dissection and placed into sterile plastic containers. Aseptic techniques were employed for sampling these specimens to avoid contamination with either contemporary H. pylori DNA or cross-contamination among samples. Four tissue samples of gastric remains, tongue-soft palate, and brain were obtained from only two mummies, in which the natural orifices found in the mummies allowed exploration of internal structures without damaging tissues or fabrics surrounding the corpses (Table 1).


Presence of Helicobacter pylori in a Mexican Pre-Columbian Mummy.

Castillo-Rojas G, Cerbón MA, López-Vidal Y - BMC Microbiol. (2008)

Mexican Pre-Columbian mummies. Yellow box showed the mummy that was Helicobacter pylori-positive.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2483284&req=5

Figure 1: Mexican Pre-Columbian mummies. Yellow box showed the mummy that was Helicobacter pylori-positive.
Mentions: Four pre-Columbian mummified bodies recovered in the funeral cave of La Ventana, located in the Chihuahua State desert and two additional mummies recovered in a cave located in the state of Durango (Figure 1) were studied. Climatic conditions in the caves allowed natural preservation of the bodies throughout centuries. (We are grateful to Dr. Josefina Mansilla at Instituto de Antropología e Historia de México who allowed us to obtain mummy samples). The samples were studied to obtain valuable information on the soft tissues and for internal-organ visualization using a Pentax EG-1840 gastroscope, a Pentax VB-1530 videobronchoscope, and a Pentax ELC-2800 videolaparoscope. (We kindly appreciate the support from Dr. Fernando Mundo of the Hospital Angeles del Pedregal in Mexico City for sample collection). Sampling was carried out in a room with no history of work with H. pylori at the Instituto de Antropología e Historia de México. Tissue specimens from the mummies were acquired by dissection and placed into sterile plastic containers. Aseptic techniques were employed for sampling these specimens to avoid contamination with either contemporary H. pylori DNA or cross-contamination among samples. Four tissue samples of gastric remains, tongue-soft palate, and brain were obtained from only two mummies, in which the natural orifices found in the mummies allowed exploration of internal structures without damaging tissues or fabrics surrounding the corpses (Table 1).

Bottom Line: These PCR products were hybridized with a pHp probe.Nucleotide sequence analysis showed homology with H. pylori in 98 of 99% when compared with the gene bank nucleotide sequence.Only one sample of gastric remnant H. pylori-positive with 16S rRNA gene was also positive for ureB gene from H. pylori.

View Article: PubMed Central - HTML - PubMed

Affiliation: Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico. gcastillo55@hotmail.com

ABSTRACT

Background: Recent studies showed that Helicobacter pylori existed in the New World prior to the arrival of Columbus. The purpose of the present study was to detect the presence of Helicobacter pylori in pre-Columbian mummies from Northern Mexico.

Methods: Six samples were studied (four samples of gastric remains, tongue-soft palate, and brain remained as negative controls) from two of the six naturally mummified corpses studied (adult male and infant male). Samples were taken from tissues suitable for DNA amplification by Polymerase chain reaction (PCR). DNA was extracted and H. pylori detection was carried out by PCR and hybridized with the pHp probe from 16S rRNA gene. The purified PCR products were cloned and sequenced in both directions. DNA sequences were analyzed with ALIGN and BLAST software. A second amplification was performed using ureB gene by real-time PCR.

Results: From four samples of gastric remnant, only two were H. pylori-positive for amplification of a 109 bp DNA fragment; the remaining two were negative, as were the tongue-soft palate and the brain biopsies as well. These PCR products were hybridized with a pHp probe. Nucleotide sequence analysis showed homology with H. pylori in 98 of 99% when compared with the gene bank nucleotide sequence. Only one sample of gastric remnant H. pylori-positive with 16S rRNA gene was also positive for ureB gene from H. pylori.

Conclusion: This data supported infection with H. pylori in Mexican pre-Columbian mummies dating from approximately 1,350 AC.

Show MeSH
Related in: MedlinePlus