Limits...
Unsaturated long-chain fatty acids induce the respiratory burst of human neutrophils and monocytes in whole blood.

Jüttner B, Kröplin J, Coldewey SM, Witt L, Osthaus WA, Weilbach C, Scheinichen D - Nutr Metab (Lond) (2008)

Bottom Line: Whole blood samples were incubated with LCT (Intralipid(R)), LCT/MCT (Lipofundin(R)) and LCT-MUFA (ClinOleic(R)) in three concentrations (0.06, 0.3 and 0.6 mg ml-1) for time periods up to one hour.We concluded that parenteral nutrition containing unsaturated oleic (C18:1) and linoleic (C18:2) acid can induce respiratory burst of neutrophils and monocytes, resulting in an elevated risk of tissue damage by the uncontrolled production of reactive oxygen species.Contradictory observations reported in previous studies may in part be the result of different methods used to determine hydrogen peroxide production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Anaesthesiology, Hannover Medical School, Germany. juettner.bjoern@mh-hannover.de.

ABSTRACT

Background: It is increasingly recognized that infectious complications in patients treated with total parenteral nutrition (TPN) may be caused by altered immune responses. Neutrophils and monocytes are the first line of defence against bacterial and fungal infection through superoxide anion production during the respiratory burst. To characterize the impact of three different types of lipid solutions that are applied as part of TPN formulations, we investigated the unstimulated respiratory burst activation of neutrophils and monocytes in whole blood.

Methods: Whole blood samples were incubated with LCT (Intralipid(R)), LCT/MCT (Lipofundin(R)) and LCT-MUFA (ClinOleic(R)) in three concentrations (0.06, 0.3 and 0.6 mg ml-1) for time periods up to one hour. Hydrogen peroxide production during the respiratory burst of neutrophils and monocytes was measured by flow cytometry.

Results: LCT and LCT-MUFA induced a hydrogen peroxide production in neutrophils and monocytes without presence of a physiological stimulus in contrast to LCT/MCT.

Conclusion: We concluded that parenteral nutrition containing unsaturated oleic (C18:1) and linoleic (C18:2) acid can induce respiratory burst of neutrophils and monocytes, resulting in an elevated risk of tissue damage by the uncontrolled production of reactive oxygen species. Contradictory observations reported in previous studies may in part be the result of different methods used to determine hydrogen peroxide production.

No MeSH data available.


Related in: MedlinePlus

Time course of neutrophils having produced hydrogen peroxide. (●) control without lipid emulsions and incubation with (A) 0.06 mg ml-1, (B) 0.3 mg ml-1 and (C) 0.6 mg ml-1 of (▼) LCT/MCT, (■) LCT-MUFA and (◆) LCT. *p < 0.05 lipid emulsion versus negative control, post hoc Dunnett-t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC2483276&req=5

Figure 2: Time course of neutrophils having produced hydrogen peroxide. (●) control without lipid emulsions and incubation with (A) 0.06 mg ml-1, (B) 0.3 mg ml-1 and (C) 0.6 mg ml-1 of (▼) LCT/MCT, (■) LCT-MUFA and (◆) LCT. *p < 0.05 lipid emulsion versus negative control, post hoc Dunnett-t-test.

Mentions: Alterations of the hydrogen peroxide production of phagocytes depended on the lipid group and on the concentration of the lipid emulsion, Figure 1. Exposure to LCT/MCT resulted in no significant differences of neutrophil and monocyte hydrogen peroxide production compared to negative control values, Figure 2, Table 2 and 3 (see additional file 1). Incubation with LCT-MUFA or LCT was associated with significant changes in the rhodamine fluorescence signal and side scatter characteristics of a subpopulation of neutrophils.


Unsaturated long-chain fatty acids induce the respiratory burst of human neutrophils and monocytes in whole blood.

Jüttner B, Kröplin J, Coldewey SM, Witt L, Osthaus WA, Weilbach C, Scheinichen D - Nutr Metab (Lond) (2008)

Time course of neutrophils having produced hydrogen peroxide. (●) control without lipid emulsions and incubation with (A) 0.06 mg ml-1, (B) 0.3 mg ml-1 and (C) 0.6 mg ml-1 of (▼) LCT/MCT, (■) LCT-MUFA and (◆) LCT. *p < 0.05 lipid emulsion versus negative control, post hoc Dunnett-t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2483276&req=5

Figure 2: Time course of neutrophils having produced hydrogen peroxide. (●) control without lipid emulsions and incubation with (A) 0.06 mg ml-1, (B) 0.3 mg ml-1 and (C) 0.6 mg ml-1 of (▼) LCT/MCT, (■) LCT-MUFA and (◆) LCT. *p < 0.05 lipid emulsion versus negative control, post hoc Dunnett-t-test.
Mentions: Alterations of the hydrogen peroxide production of phagocytes depended on the lipid group and on the concentration of the lipid emulsion, Figure 1. Exposure to LCT/MCT resulted in no significant differences of neutrophil and monocyte hydrogen peroxide production compared to negative control values, Figure 2, Table 2 and 3 (see additional file 1). Incubation with LCT-MUFA or LCT was associated with significant changes in the rhodamine fluorescence signal and side scatter characteristics of a subpopulation of neutrophils.

Bottom Line: Whole blood samples were incubated with LCT (Intralipid(R)), LCT/MCT (Lipofundin(R)) and LCT-MUFA (ClinOleic(R)) in three concentrations (0.06, 0.3 and 0.6 mg ml-1) for time periods up to one hour.We concluded that parenteral nutrition containing unsaturated oleic (C18:1) and linoleic (C18:2) acid can induce respiratory burst of neutrophils and monocytes, resulting in an elevated risk of tissue damage by the uncontrolled production of reactive oxygen species.Contradictory observations reported in previous studies may in part be the result of different methods used to determine hydrogen peroxide production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Anaesthesiology, Hannover Medical School, Germany. juettner.bjoern@mh-hannover.de.

ABSTRACT

Background: It is increasingly recognized that infectious complications in patients treated with total parenteral nutrition (TPN) may be caused by altered immune responses. Neutrophils and monocytes are the first line of defence against bacterial and fungal infection through superoxide anion production during the respiratory burst. To characterize the impact of three different types of lipid solutions that are applied as part of TPN formulations, we investigated the unstimulated respiratory burst activation of neutrophils and monocytes in whole blood.

Methods: Whole blood samples were incubated with LCT (Intralipid(R)), LCT/MCT (Lipofundin(R)) and LCT-MUFA (ClinOleic(R)) in three concentrations (0.06, 0.3 and 0.6 mg ml-1) for time periods up to one hour. Hydrogen peroxide production during the respiratory burst of neutrophils and monocytes was measured by flow cytometry.

Results: LCT and LCT-MUFA induced a hydrogen peroxide production in neutrophils and monocytes without presence of a physiological stimulus in contrast to LCT/MCT.

Conclusion: We concluded that parenteral nutrition containing unsaturated oleic (C18:1) and linoleic (C18:2) acid can induce respiratory burst of neutrophils and monocytes, resulting in an elevated risk of tissue damage by the uncontrolled production of reactive oxygen species. Contradictory observations reported in previous studies may in part be the result of different methods used to determine hydrogen peroxide production.

No MeSH data available.


Related in: MedlinePlus