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Autoantigenic nuclear proteins of a clinically atypical renal vasculitis.

Avila J, Acosta E, Machargo MD, Arteaga MF, Gallego E, Cañete H, García-Pérez JJ, Martín-Vasallo P - J Autoimmune Dis (2008)

Bottom Line: None of them had been reported as autoantigen.Only the serum from the patient origin of this study recognized all recombinant proteins.We identify four nuclear proteins, HDAC5, TFC4, RTF1 and POLDIP3 polymerase as new autoantigens that could be used as markers in the diagnosis of subfamilies in immune diseases, although we cannot determine the role of these proteins in the aetiopathogenic process.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Bioquímica y Biología Molecular, Laboratorio de Biología del Desarrollo, Universidad de La Laguna, 38206 La Laguna, Tenerife, Spain.

ABSTRACT

Background: Systemic vasculitides constitute a heterogeneous group of diseases of autoimmunological origin characterized by inflammation of blood vessels and antibodies that react against autoantigens in a process that ultimately affects blood vessel walls. An important number of these patients present kidney disease. An endeavour of this area of research is the identification of autoantigens involved in these diseases. Accordingly, we used serum from a patient suffering from a microscopic polyangiitis, P-ANCA positive, manifesting a clinically atypical renal necrotizing glomerulonephritis and interstitial nephropathy for the identification of autoantigens; we also determined the prevalence of corresponding autoantibodies in other vasculitides, diabetic microangiopathy and in general population.

Methods: The patient's serum was used as a probe for the immunoscreening method SEREX to screen a human brain cDNA expression library.

Results: Four positive clones were isolated and sequenced. Clones Jos002 code for protein HDAC5, Jos014 for TFC4, Jos107 for RTF1, and Jos313 for POLDIP3 polymerase. The four proteins are of nuclear localization. None of them had been reported as autoantigen. Recombinant proteins were synthesised and checked as antigens by western blot with different sera from controls and patients affected with other vasculitides and diabetic microangiopathy as well. Only the serum from the patient origin of this study recognized all recombinant proteins.

Conclusion: We identify four nuclear proteins, HDAC5, TFC4, RTF1 and POLDIP3 polymerase as new autoantigens that could be used as markers in the diagnosis of subfamilies in immune diseases, although we cannot determine the role of these proteins in the aetiopathogenic process.

No MeSH data available.


Related in: MedlinePlus

Virtual northern blot of genes coding for autoantigens reported in this study. Horizontal axis, tissues and cell lines; vertical axis, expression level of the given gene in arbitrary units of intensity.
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Figure 1: Virtual northern blot of genes coding for autoantigens reported in this study. Horizontal axis, tissues and cell lines; vertical axis, expression level of the given gene in arbitrary units of intensity.

Mentions: The expression pattern of these genes in human was studied by a virtual Northern-blot analysis. Figure 1 shows the searching results. HDAC5 reaches a higher expression level in placenta, brain (both, adult and foetal), and heart. TCF4 is expressed at a medium level in most tissues with the exception of PB-BDCA4+ (dendritic cell line) with a ten fold of the average expression level. RTF1 expression pattern shows the highest level in BM-CD34+ cells and medium in endothelial tissue BMCD105 and PB-BDCA4+ and T CD4+ cell lines. POLDIP3 is the most evenly distributed protein among tissues, spleen is the predominant.


Autoantigenic nuclear proteins of a clinically atypical renal vasculitis.

Avila J, Acosta E, Machargo MD, Arteaga MF, Gallego E, Cañete H, García-Pérez JJ, Martín-Vasallo P - J Autoimmune Dis (2008)

Virtual northern blot of genes coding for autoantigens reported in this study. Horizontal axis, tissues and cell lines; vertical axis, expression level of the given gene in arbitrary units of intensity.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2483274&req=5

Figure 1: Virtual northern blot of genes coding for autoantigens reported in this study. Horizontal axis, tissues and cell lines; vertical axis, expression level of the given gene in arbitrary units of intensity.
Mentions: The expression pattern of these genes in human was studied by a virtual Northern-blot analysis. Figure 1 shows the searching results. HDAC5 reaches a higher expression level in placenta, brain (both, adult and foetal), and heart. TCF4 is expressed at a medium level in most tissues with the exception of PB-BDCA4+ (dendritic cell line) with a ten fold of the average expression level. RTF1 expression pattern shows the highest level in BM-CD34+ cells and medium in endothelial tissue BMCD105 and PB-BDCA4+ and T CD4+ cell lines. POLDIP3 is the most evenly distributed protein among tissues, spleen is the predominant.

Bottom Line: None of them had been reported as autoantigen.Only the serum from the patient origin of this study recognized all recombinant proteins.We identify four nuclear proteins, HDAC5, TFC4, RTF1 and POLDIP3 polymerase as new autoantigens that could be used as markers in the diagnosis of subfamilies in immune diseases, although we cannot determine the role of these proteins in the aetiopathogenic process.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Bioquímica y Biología Molecular, Laboratorio de Biología del Desarrollo, Universidad de La Laguna, 38206 La Laguna, Tenerife, Spain.

ABSTRACT

Background: Systemic vasculitides constitute a heterogeneous group of diseases of autoimmunological origin characterized by inflammation of blood vessels and antibodies that react against autoantigens in a process that ultimately affects blood vessel walls. An important number of these patients present kidney disease. An endeavour of this area of research is the identification of autoantigens involved in these diseases. Accordingly, we used serum from a patient suffering from a microscopic polyangiitis, P-ANCA positive, manifesting a clinically atypical renal necrotizing glomerulonephritis and interstitial nephropathy for the identification of autoantigens; we also determined the prevalence of corresponding autoantibodies in other vasculitides, diabetic microangiopathy and in general population.

Methods: The patient's serum was used as a probe for the immunoscreening method SEREX to screen a human brain cDNA expression library.

Results: Four positive clones were isolated and sequenced. Clones Jos002 code for protein HDAC5, Jos014 for TFC4, Jos107 for RTF1, and Jos313 for POLDIP3 polymerase. The four proteins are of nuclear localization. None of them had been reported as autoantigen. Recombinant proteins were synthesised and checked as antigens by western blot with different sera from controls and patients affected with other vasculitides and diabetic microangiopathy as well. Only the serum from the patient origin of this study recognized all recombinant proteins.

Conclusion: We identify four nuclear proteins, HDAC5, TFC4, RTF1 and POLDIP3 polymerase as new autoantigens that could be used as markers in the diagnosis of subfamilies in immune diseases, although we cannot determine the role of these proteins in the aetiopathogenic process.

No MeSH data available.


Related in: MedlinePlus