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Genetic diversity among Turkish native chickens, Denizli and Gerze, estimated by microsatellite markers.

Kaya M, Yildiz MA - Biochem. Genet. (2008)

Bottom Line: Its topology reflects the general pattern of genetic differentiation among the Denizli and Gerze breeds.The present study suggests that Denizli and Gerze subpopulations have a rich genetic diversity.The information about Denizli and Gerze breeds estimated by microsatellite analysis may also be useful as an initial guide in defining objectives for designing future investigations of genetic variation and developing conservation strategies.

View Article: PubMed Central - PubMed

Affiliation: Poultry Research Institute, Pb 47, Yenimahalle, Ankara, Turkey.

ABSTRACT
The genetic diversity of the Turkish native chicken breeds Denizli and Gerze was evaluated with 10 microsatellite markers. We genotyped a total of 125 individuals from five subpopulations. Among loci, the mean number of alleles was 7.5, expected heterozygosity (H (e)) was 0.665, PIC value was 0.610, and Wright's fixation index was 0.301. H (e) was higher in the Denizli breed (0.656) than in the Gerze breed (0.475). The PIC values were 0.599 and 0.426 for Denizli and Gerze, respectively. A phylogenetic tree was constructed using genetic distance and the neighbor-joining method. Its topology reflects the general pattern of genetic differentiation among the Denizli and Gerze breeds. The present study suggests that Denizli and Gerze subpopulations have a rich genetic diversity. The information about Denizli and Gerze breeds estimated by microsatellite analysis may also be useful as an initial guide in defining objectives for designing future investigations of genetic variation and developing conservation strategies.

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Neighbor-joining dendrogram among five subpopulations of the Denizli and Gerze chicken breeds. Based on Nei’s (1978) unbiased genetic distances from Table 3
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Fig1: Neighbor-joining dendrogram among five subpopulations of the Denizli and Gerze chicken breeds. Based on Nei’s (1978) unbiased genetic distances from Table 3

Mentions: Using Nei’s (1978) unbiased genetic distance (Table 3) and the neighbor-joining method, a phylogenetic tree was constructed for the Denizli and Gerze subpopulations. The smallest genetic distance, between DLHMAE and DHUC, was quite low (0.0652). A similar result was obtained for the GLMHAE and GOI subpopulations, with a very low genetic distance (0.0783). The larger genetic distances (greater than 0.4193) were found between the Denizli and Gerze subpopulations. The neighbor-joining dendrogram in Fig. 1 was drawn using the genetic distances given in Table 3. The Denizli (DHUC, DLHMAE, and DOI) and Gerze (GLHMAE and GOI) breeds were clearly clustered as different groups according to their origin, supporting the reliability of this analysis.Table 3


Genetic diversity among Turkish native chickens, Denizli and Gerze, estimated by microsatellite markers.

Kaya M, Yildiz MA - Biochem. Genet. (2008)

Neighbor-joining dendrogram among five subpopulations of the Denizli and Gerze chicken breeds. Based on Nei’s (1978) unbiased genetic distances from Table 3
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2480610&req=5

Fig1: Neighbor-joining dendrogram among five subpopulations of the Denizli and Gerze chicken breeds. Based on Nei’s (1978) unbiased genetic distances from Table 3
Mentions: Using Nei’s (1978) unbiased genetic distance (Table 3) and the neighbor-joining method, a phylogenetic tree was constructed for the Denizli and Gerze subpopulations. The smallest genetic distance, between DLHMAE and DHUC, was quite low (0.0652). A similar result was obtained for the GLMHAE and GOI subpopulations, with a very low genetic distance (0.0783). The larger genetic distances (greater than 0.4193) were found between the Denizli and Gerze subpopulations. The neighbor-joining dendrogram in Fig. 1 was drawn using the genetic distances given in Table 3. The Denizli (DHUC, DLHMAE, and DOI) and Gerze (GLHMAE and GOI) breeds were clearly clustered as different groups according to their origin, supporting the reliability of this analysis.Table 3

Bottom Line: Its topology reflects the general pattern of genetic differentiation among the Denizli and Gerze breeds.The present study suggests that Denizli and Gerze subpopulations have a rich genetic diversity.The information about Denizli and Gerze breeds estimated by microsatellite analysis may also be useful as an initial guide in defining objectives for designing future investigations of genetic variation and developing conservation strategies.

View Article: PubMed Central - PubMed

Affiliation: Poultry Research Institute, Pb 47, Yenimahalle, Ankara, Turkey.

ABSTRACT
The genetic diversity of the Turkish native chicken breeds Denizli and Gerze was evaluated with 10 microsatellite markers. We genotyped a total of 125 individuals from five subpopulations. Among loci, the mean number of alleles was 7.5, expected heterozygosity (H (e)) was 0.665, PIC value was 0.610, and Wright's fixation index was 0.301. H (e) was higher in the Denizli breed (0.656) than in the Gerze breed (0.475). The PIC values were 0.599 and 0.426 for Denizli and Gerze, respectively. A phylogenetic tree was constructed using genetic distance and the neighbor-joining method. Its topology reflects the general pattern of genetic differentiation among the Denizli and Gerze breeds. The present study suggests that Denizli and Gerze subpopulations have a rich genetic diversity. The information about Denizli and Gerze breeds estimated by microsatellite analysis may also be useful as an initial guide in defining objectives for designing future investigations of genetic variation and developing conservation strategies.

Show MeSH
Related in: MedlinePlus