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Peripheral monocytes from diabetic patients with coronary artery disease display increased bFGF and VEGF mRNA expression.

Panutsopulos D, Zafiropoulos A, Krambovitis E, Kochiadakis GE, Igoumenidis NE, Spandidos DA - J Transl Med (2003)

Bottom Line: Seventeen were found to be healthy and 36 were diagnosed with CAD.RESULTS: The statistical analysis revealed a significant increase of the basal level expression for macrophage VEGF and bFGF in the CAD SA (stable angina) patient group compared to the noCAD (control) (p = 0.041 and p = 0.022 respectively) and CAD UA (unstable angina) (p = 0.024 and p = 0.005 respectively) groups, which was highly dependent on the diabetic status of the population.The results give new insight to CAD and the impaired collateral vessel formation in diabetics.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Medical School, University of Crete, Heraklion, Crete, Greece. spandidos@spandidos.gr

ABSTRACT
BACKGROUND: Macrophages can produce vascular endothelial growth factor (VEGF) in response to hypoxia, transforming growth factor beta1 (TGF-beta1), angiotensin II, basic fibroblast growth factor (bFGF), and interleukin-1. These factors have been found in the serum of coronary artery disease (CAD) patients as well as in atherosclerotic lesions. The aim of the present study was to test the hypothesis that the expression of VEGF, TGF-beta1 and bFGF in peripheral monocytes and lymphocytes is related to CAD. METHODS: Human Mononuclear cells and lymphocytes from peripheral blood were isolated from 53 donors undergoing angiography. Seventeen were found to be healthy and 36 were diagnosed with CAD. The respective mRNAs were extracted and quantified. RESULTS: The statistical analysis revealed a significant increase of the basal level expression for macrophage VEGF and bFGF in the CAD SA (stable angina) patient group compared to the noCAD (control) (p = 0.041 and p = 0.022 respectively) and CAD UA (unstable angina) (p = 0.024 and p = 0.005 respectively) groups, which was highly dependent on the diabetic status of the population. Furthermore, we demonstrated with an in vitro cell culture model that the levels of VEGF and bFGF in monocytes of healthy donors are not affected by short term exposure to increased glucose levels (usually observed in the diabetic patients) and/or statin. CONCLUSION: Our findings display a statistically significant association of the increased VEGF and bFGF levels in peripheral monocytes, with stable angina and diabetes in coronary artery disease. The results give new insight to CAD and the impaired collateral vessel formation in diabetics.

No MeSH data available.


Related in: MedlinePlus

Boxplots of the expression of VEGF and bFGF. Distribution boxplot of the expression of VEGF in monocytes/macrophages (A, B), bFGF in monocytes /macrophages (C, D) and VEGF in lymphocytes (E, F). The boxplots A, C and E demonstrate the expression in the control, CAD SA and CAD UA group. The boxplots B, D and F demonstrate the expression in the above groups classified for diabetes. The y-axis values represent the VEGF/β2-microglobulin and bFGF/β2-microglobulin ratios accordingly. The ratios were calculated by the optical integrated density of each gene divided by that of β2-microglobulin. The optical integrated density was calculated by digital image analysis (Scion image) of acrylamide gel electrophoresis of the RT-PCR products. Mf: macrophages, Ly: non-adherent lymphocyte fraction, ns: non-significant.
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Figure 3: Boxplots of the expression of VEGF and bFGF. Distribution boxplot of the expression of VEGF in monocytes/macrophages (A, B), bFGF in monocytes /macrophages (C, D) and VEGF in lymphocytes (E, F). The boxplots A, C and E demonstrate the expression in the control, CAD SA and CAD UA group. The boxplots B, D and F demonstrate the expression in the above groups classified for diabetes. The y-axis values represent the VEGF/β2-microglobulin and bFGF/β2-microglobulin ratios accordingly. The ratios were calculated by the optical integrated density of each gene divided by that of β2-microglobulin. The optical integrated density was calculated by digital image analysis (Scion image) of acrylamide gel electrophoresis of the RT-PCR products. Mf: macrophages, Ly: non-adherent lymphocyte fraction, ns: non-significant.

Mentions: The mRNA quantification revealed a significant difference in the basal level expression between the three groups for macrophage VEGF and bFGF(Figure 3A,3C). No statistically significant differences were detected for macrophage TGF-β1 and lymphocyte VEGF, bFGF and TGF-β1.


Peripheral monocytes from diabetic patients with coronary artery disease display increased bFGF and VEGF mRNA expression.

Panutsopulos D, Zafiropoulos A, Krambovitis E, Kochiadakis GE, Igoumenidis NE, Spandidos DA - J Transl Med (2003)

Boxplots of the expression of VEGF and bFGF. Distribution boxplot of the expression of VEGF in monocytes/macrophages (A, B), bFGF in monocytes /macrophages (C, D) and VEGF in lymphocytes (E, F). The boxplots A, C and E demonstrate the expression in the control, CAD SA and CAD UA group. The boxplots B, D and F demonstrate the expression in the above groups classified for diabetes. The y-axis values represent the VEGF/β2-microglobulin and bFGF/β2-microglobulin ratios accordingly. The ratios were calculated by the optical integrated density of each gene divided by that of β2-microglobulin. The optical integrated density was calculated by digital image analysis (Scion image) of acrylamide gel electrophoresis of the RT-PCR products. Mf: macrophages, Ly: non-adherent lymphocyte fraction, ns: non-significant.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC239962&req=5

Figure 3: Boxplots of the expression of VEGF and bFGF. Distribution boxplot of the expression of VEGF in monocytes/macrophages (A, B), bFGF in monocytes /macrophages (C, D) and VEGF in lymphocytes (E, F). The boxplots A, C and E demonstrate the expression in the control, CAD SA and CAD UA group. The boxplots B, D and F demonstrate the expression in the above groups classified for diabetes. The y-axis values represent the VEGF/β2-microglobulin and bFGF/β2-microglobulin ratios accordingly. The ratios were calculated by the optical integrated density of each gene divided by that of β2-microglobulin. The optical integrated density was calculated by digital image analysis (Scion image) of acrylamide gel electrophoresis of the RT-PCR products. Mf: macrophages, Ly: non-adherent lymphocyte fraction, ns: non-significant.
Mentions: The mRNA quantification revealed a significant difference in the basal level expression between the three groups for macrophage VEGF and bFGF(Figure 3A,3C). No statistically significant differences were detected for macrophage TGF-β1 and lymphocyte VEGF, bFGF and TGF-β1.

Bottom Line: Seventeen were found to be healthy and 36 were diagnosed with CAD.RESULTS: The statistical analysis revealed a significant increase of the basal level expression for macrophage VEGF and bFGF in the CAD SA (stable angina) patient group compared to the noCAD (control) (p = 0.041 and p = 0.022 respectively) and CAD UA (unstable angina) (p = 0.024 and p = 0.005 respectively) groups, which was highly dependent on the diabetic status of the population.The results give new insight to CAD and the impaired collateral vessel formation in diabetics.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Virology, Medical School, University of Crete, Heraklion, Crete, Greece. spandidos@spandidos.gr

ABSTRACT
BACKGROUND: Macrophages can produce vascular endothelial growth factor (VEGF) in response to hypoxia, transforming growth factor beta1 (TGF-beta1), angiotensin II, basic fibroblast growth factor (bFGF), and interleukin-1. These factors have been found in the serum of coronary artery disease (CAD) patients as well as in atherosclerotic lesions. The aim of the present study was to test the hypothesis that the expression of VEGF, TGF-beta1 and bFGF in peripheral monocytes and lymphocytes is related to CAD. METHODS: Human Mononuclear cells and lymphocytes from peripheral blood were isolated from 53 donors undergoing angiography. Seventeen were found to be healthy and 36 were diagnosed with CAD. The respective mRNAs were extracted and quantified. RESULTS: The statistical analysis revealed a significant increase of the basal level expression for macrophage VEGF and bFGF in the CAD SA (stable angina) patient group compared to the noCAD (control) (p = 0.041 and p = 0.022 respectively) and CAD UA (unstable angina) (p = 0.024 and p = 0.005 respectively) groups, which was highly dependent on the diabetic status of the population. Furthermore, we demonstrated with an in vitro cell culture model that the levels of VEGF and bFGF in monocytes of healthy donors are not affected by short term exposure to increased glucose levels (usually observed in the diabetic patients) and/or statin. CONCLUSION: Our findings display a statistically significant association of the increased VEGF and bFGF levels in peripheral monocytes, with stable angina and diabetes in coronary artery disease. The results give new insight to CAD and the impaired collateral vessel formation in diabetics.

No MeSH data available.


Related in: MedlinePlus