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History and diagnostic significance of C-peptide.

Brandenburg D - Exp Diabetes Res (2008)

Bottom Line: Today, C-peptide continues to serve as a special diagnostic tool in Diabetology and related fields.Thus, its passive role is well established.Evidence for its active role in physiology and pathophysiology is more recent and is subject of the following contributions.

View Article: PubMed Central - PubMed

Affiliation: Institute for Clinical Research and Development, D-55116 Mainz, Germany. dietrich.brandenburg@t-online.de

ABSTRACT
Starting with the epoch-making discovery of proinsulin, C-peptide has played an important interdisciplinary role, both as part of the single-chain precursor molecule and as an individual entity. In the pioneering years, fundamental systematic experiments unravelled new biochemical mechanisms and chemical structures. After the first detection of C-peptide in human serum, it quickly became a most useful independent indicator of insulin biosynthesis and secretion, finding application in a rapidly growing number of clinical investigations. A prerequisite was the development of specific immuno assays for proinsulin and C-peptide. Further milestones were: the chemical synthesis of several C-peptides and the accomplishments in the synthesis of proinsulin; the detection of preproinsulin with its bearings on understanding protein biosynthesis; the pioneering role of insulin, proinsulin, C-peptide, and mini-C-peptides in the development of recombinant DNA technology; and the discovery of the enzymes for the endoproteolytic processing of proinsulin into insulin and C-peptide, completing the pathway of biosynthesis. Today, C-peptide continues to serve as a special diagnostic tool in Diabetology and related fields. Thus, its passive role is well established. Evidence for its active role in physiology and pathophysiology is more recent and is subject of the following contributions.

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Mentions: In 1968, Chance et al. [5] isolate and characterise proinsulinfrom crystalline porcine insulin. They elucidate the amino acid sequence of a33-peptide, which links the insulin chains and is designated “connectingpeptide.” Thus, the findings and concept of Steiner are confirmed and extended,and all future work can now be based on solid protein-chemical grounds. Figure 2 depicts the structure of C-peptide and shows the sequence of human C-peptide.


History and diagnostic significance of C-peptide.

Brandenburg D - Exp Diabetes Res (2008)

© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2396242&req=5

Mentions: In 1968, Chance et al. [5] isolate and characterise proinsulinfrom crystalline porcine insulin. They elucidate the amino acid sequence of a33-peptide, which links the insulin chains and is designated “connectingpeptide.” Thus, the findings and concept of Steiner are confirmed and extended,and all future work can now be based on solid protein-chemical grounds. Figure 2 depicts the structure of C-peptide and shows the sequence of human C-peptide.

Bottom Line: Today, C-peptide continues to serve as a special diagnostic tool in Diabetology and related fields.Thus, its passive role is well established.Evidence for its active role in physiology and pathophysiology is more recent and is subject of the following contributions.

View Article: PubMed Central - PubMed

Affiliation: Institute for Clinical Research and Development, D-55116 Mainz, Germany. dietrich.brandenburg@t-online.de

ABSTRACT
Starting with the epoch-making discovery of proinsulin, C-peptide has played an important interdisciplinary role, both as part of the single-chain precursor molecule and as an individual entity. In the pioneering years, fundamental systematic experiments unravelled new biochemical mechanisms and chemical structures. After the first detection of C-peptide in human serum, it quickly became a most useful independent indicator of insulin biosynthesis and secretion, finding application in a rapidly growing number of clinical investigations. A prerequisite was the development of specific immuno assays for proinsulin and C-peptide. Further milestones were: the chemical synthesis of several C-peptides and the accomplishments in the synthesis of proinsulin; the detection of preproinsulin with its bearings on understanding protein biosynthesis; the pioneering role of insulin, proinsulin, C-peptide, and mini-C-peptides in the development of recombinant DNA technology; and the discovery of the enzymes for the endoproteolytic processing of proinsulin into insulin and C-peptide, completing the pathway of biosynthesis. Today, C-peptide continues to serve as a special diagnostic tool in Diabetology and related fields. Thus, its passive role is well established. Evidence for its active role in physiology and pathophysiology is more recent and is subject of the following contributions.

Show MeSH
Related in: MedlinePlus