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Combining X-ray and electron-microscopy data to solve crystal structures.

Navaza J - Acta Crystallogr. D Biol. Crystallogr. (2007)

Bottom Line: Low-resolution electron-microscopy reconstructions can be used as search models in molecular replacement or may be combined with existing monomeric structures in order to produce multimeric models suitable for molecular replacement.The technique is described in the case of viral and subviral particles as well as in the case of oligomeric proteins.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratoire de Microscopie Electronique Structurale, Institut de Biologie Structurale Jean-Pierre Ebel, 41 Rue Jules Horowitz, F-38027 Grenoble, France. jorge.navaza@ibs.fr

ABSTRACT
Low-resolution electron-microscopy reconstructions can be used as search models in molecular replacement or may be combined with existing monomeric structures in order to produce multimeric models suitable for molecular replacement. The technique is described in the case of viral and subviral particles as well as in the case of oligomeric proteins.

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Fit of VP2 trimers into the cryo-TEM reconstruction of the T = 1 subviral particle. View along a threefold axis.
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fig3: Fit of VP2 trimers into the cryo-TEM reconstruction of the T = 1 subviral particle. View along a threefold axis.

Mentions: In Fig. 3 ▶, we show the results of applying URO to the T = 1 subviral particle of IBDV. The independent molecular model was taken as the VP2 trimer. 20-fold symmetry was imposed during the fitting procedure. The resulting correlation between the complex Fourier coefficients of the EM and the model based maps is 77.9% when using data in the 400–20 Å resolution range.


Combining X-ray and electron-microscopy data to solve crystal structures.

Navaza J - Acta Crystallogr. D Biol. Crystallogr. (2007)

Fit of VP2 trimers into the cryo-TEM reconstruction of the T = 1 subviral particle. View along a threefold axis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2394810&req=5

fig3: Fit of VP2 trimers into the cryo-TEM reconstruction of the T = 1 subviral particle. View along a threefold axis.
Mentions: In Fig. 3 ▶, we show the results of applying URO to the T = 1 subviral particle of IBDV. The independent molecular model was taken as the VP2 trimer. 20-fold symmetry was imposed during the fitting procedure. The resulting correlation between the complex Fourier coefficients of the EM and the model based maps is 77.9% when using data in the 400–20 Å resolution range.

Bottom Line: Low-resolution electron-microscopy reconstructions can be used as search models in molecular replacement or may be combined with existing monomeric structures in order to produce multimeric models suitable for molecular replacement.The technique is described in the case of viral and subviral particles as well as in the case of oligomeric proteins.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratoire de Microscopie Electronique Structurale, Institut de Biologie Structurale Jean-Pierre Ebel, 41 Rue Jules Horowitz, F-38027 Grenoble, France. jorge.navaza@ibs.fr

ABSTRACT
Low-resolution electron-microscopy reconstructions can be used as search models in molecular replacement or may be combined with existing monomeric structures in order to produce multimeric models suitable for molecular replacement. The technique is described in the case of viral and subviral particles as well as in the case of oligomeric proteins.

Show MeSH