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Glucose transporter-1 (GLUT-1): a potential marker of prognosis in rectal carcinoma?

Cooper R, Sarioğlu S, Sökmen S, Füzün M, Küpelioğlu A, Valentine H, Görken IB, Airley R, West C - Br. J. Cancer (2003)

Bottom Line: A diffuse and patchy pattern of staining, with and without colocalisation to necrosis was seen.No significant correlation was seen with other prognostic factors.In conclusion, increased GLUT-1 expression in rectal tumours was an adverse prognostic factor and is worth further evaluation as a predictive marker of response to therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Dokuz Eylül University Medical School, Inciraltu, Izmir 35340, Turkey. rachel.cooper@deu.edu.tr

ABSTRACT
The aim of the study is to evaluate the pattern and level of expression of glucose transporter-1 (GLUT-1) in rectal carcinoma in relation to outcome as a potential surrogate marker of tumour hypoxia. Formalin-fixed tumour sections from 43 patients with rectal carcinoma, who had undergone radical resection with curative intent, were immunohistochemically stained for GLUT-1. A mean of three sections per tumour (range 1-12) were examined. Each section was semiquantitatively scored; 0, no staining; 1, <10%; 2, 10-50%; 3, >50% and a score given for the whole section, the superficial (luminal) and deep (mural) part of the tumour. Staining was seen in 70% of tumours. Increased staining was noted adjacent to necrosis and ulceration. A diffuse and patchy pattern of staining, with and without colocalisation to necrosis was seen. Patients with high GLUT-1-expressing tumours (score 3 vs 0-2) had a significantly poorer overall survival (P=0.041), which was associated with poorer metastasis-free survival with no difference in local control. No significant correlation was seen with other prognostic factors. There was a strong correlation between the score for the superficial and deep parts of the tumour (r=0.81), but a significant relationship with outcome was only found in the deep part (P=0.003 vs P=0.46). In conclusion, increased GLUT-1 expression in rectal tumours was an adverse prognostic factor and is worth further evaluation as a predictive marker of response to therapy.

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(A) Score 1. Less than 10% of the cells are stained by anti-Glut-1 (magnification × 10). (B) Score 2. Between 10 and 50% of the cells are stained with Glut-1. Strong membranous staining is seen adjacent to an area of necosis. (magnification × 20). (C) Score 3. More than 50% of cells are stained with Glut-1 at the invasive border. (magnification × 20)
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fig1: (A) Score 1. Less than 10% of the cells are stained by anti-Glut-1 (magnification × 10). (B) Score 2. Between 10 and 50% of the cells are stained with Glut-1. Strong membranous staining is seen adjacent to an area of necosis. (magnification × 20). (C) Score 3. More than 50% of cells are stained with Glut-1 at the invasive border. (magnification × 20)

Mentions: Glucose transporter-1 expression was only observed as membranous staining in tumour cells. Erythrocytes within blood vessels were also seen to stain strongly for Glut-1, which served as an internal control. Perinecrotic and periulcerative areas stained strongly. Two patterns of staining were observed, a focal pattern and a more diffuse staining. Although staining was increased around necrotic areas, the patchy staining pattern was not consistently localised to necrosis. In addition, more cells were positive in the deeper portion of the tumour compared to the superfical luminal part. Normal cells and tumour stroma did not stain for Glut-1. There was a significant correlation between the first and the second score for the general (r=0.87, P<0.0001), superficial (r=0.59 P=0.03) and deep (r=0.82, P<0.0001) parts of the tumour. Figure 1Figure 1


Glucose transporter-1 (GLUT-1): a potential marker of prognosis in rectal carcinoma?

Cooper R, Sarioğlu S, Sökmen S, Füzün M, Küpelioğlu A, Valentine H, Görken IB, Airley R, West C - Br. J. Cancer (2003)

(A) Score 1. Less than 10% of the cells are stained by anti-Glut-1 (magnification × 10). (B) Score 2. Between 10 and 50% of the cells are stained with Glut-1. Strong membranous staining is seen adjacent to an area of necosis. (magnification × 20). (C) Score 3. More than 50% of cells are stained with Glut-1 at the invasive border. (magnification × 20)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2394489&req=5

fig1: (A) Score 1. Less than 10% of the cells are stained by anti-Glut-1 (magnification × 10). (B) Score 2. Between 10 and 50% of the cells are stained with Glut-1. Strong membranous staining is seen adjacent to an area of necosis. (magnification × 20). (C) Score 3. More than 50% of cells are stained with Glut-1 at the invasive border. (magnification × 20)
Mentions: Glucose transporter-1 expression was only observed as membranous staining in tumour cells. Erythrocytes within blood vessels were also seen to stain strongly for Glut-1, which served as an internal control. Perinecrotic and periulcerative areas stained strongly. Two patterns of staining were observed, a focal pattern and a more diffuse staining. Although staining was increased around necrotic areas, the patchy staining pattern was not consistently localised to necrosis. In addition, more cells were positive in the deeper portion of the tumour compared to the superfical luminal part. Normal cells and tumour stroma did not stain for Glut-1. There was a significant correlation between the first and the second score for the general (r=0.87, P<0.0001), superficial (r=0.59 P=0.03) and deep (r=0.82, P<0.0001) parts of the tumour. Figure 1Figure 1

Bottom Line: A diffuse and patchy pattern of staining, with and without colocalisation to necrosis was seen.No significant correlation was seen with other prognostic factors.In conclusion, increased GLUT-1 expression in rectal tumours was an adverse prognostic factor and is worth further evaluation as a predictive marker of response to therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiation Oncology, Dokuz Eylül University Medical School, Inciraltu, Izmir 35340, Turkey. rachel.cooper@deu.edu.tr

ABSTRACT
The aim of the study is to evaluate the pattern and level of expression of glucose transporter-1 (GLUT-1) in rectal carcinoma in relation to outcome as a potential surrogate marker of tumour hypoxia. Formalin-fixed tumour sections from 43 patients with rectal carcinoma, who had undergone radical resection with curative intent, were immunohistochemically stained for GLUT-1. A mean of three sections per tumour (range 1-12) were examined. Each section was semiquantitatively scored; 0, no staining; 1, <10%; 2, 10-50%; 3, >50% and a score given for the whole section, the superficial (luminal) and deep (mural) part of the tumour. Staining was seen in 70% of tumours. Increased staining was noted adjacent to necrosis and ulceration. A diffuse and patchy pattern of staining, with and without colocalisation to necrosis was seen. Patients with high GLUT-1-expressing tumours (score 3 vs 0-2) had a significantly poorer overall survival (P=0.041), which was associated with poorer metastasis-free survival with no difference in local control. No significant correlation was seen with other prognostic factors. There was a strong correlation between the score for the superficial and deep parts of the tumour (r=0.81), but a significant relationship with outcome was only found in the deep part (P=0.003 vs P=0.46). In conclusion, increased GLUT-1 expression in rectal tumours was an adverse prognostic factor and is worth further evaluation as a predictive marker of response to therapy.

Show MeSH
Related in: MedlinePlus