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Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference.

Liu C, Chen Y, Yu X, Jin C, Xu J, Long J, Ni Q, Fu D, Jin H, Bai C - BMC Cancer (2008)

Bottom Line: Over-expression of MBD1 has been reported in human pancreatic carcinomas.We identified five proteins that were up-regulated and nine proteins that were down-regulated.Most of the identified proteins are involved in tumorigenesis, some are prognostic biomarkers for human malignant tumors.

View Article: PubMed Central - HTML - PubMed

Affiliation: Pancreatic Disease Institute, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China. blurlc@hotmail.com

ABSTRACT

Background: Methyl-CpG binding domain protein 1 (MBD1), a suppressor of gene transcription, may be involved in inactivation of tumor suppressor genes during tumorigenesis. Over-expression of MBD1 has been reported in human pancreatic carcinomas.

Methods: In this study, we established a MBD1-knock-down pancreatic cancer cell line (BxPC-3) using stable RNA interference, to compare the proteomic changes between control and MBD1-knock-down cells using two-dimensional gel electrophoresis and mass spectrometry.

Results: We identified five proteins that were up-regulated and nine proteins that were down-regulated. Most of the identified proteins are involved in tumorigenesis, some are prognostic biomarkers for human malignant tumors.

Conclusion: Our data suggest that these differential proteins may be associated with the function of MBD1, and provide some insight into the functional mechanism of MBD1 in the development of pancreatic cancer.

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Comparison of differentially expressed proteins between BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines. A, B, C, D, E: five areas of labeled protein spots on 2-DE gel. left panel: BxPC-3/MBD1-siRNA, right panel: BxPC-3/vector.
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Figure 4: Comparison of differentially expressed proteins between BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines. A, B, C, D, E: five areas of labeled protein spots on 2-DE gel. left panel: BxPC-3/MBD1-siRNA, right panel: BxPC-3/vector.

Mentions: To investigate the changes in protein expression in pancreatic cancer after MBD1 knock-down, we performed a comparative proteomic study between BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines. Fig 3 shows two representative 2-DE maps from BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines in the pH range 4–7. Among the spots investigated, almost 30 proteins were differential expressed. But some of them were unknown proteins that could not be identified, and some of them with different PI and MW finally proved to be the same protein (such as 348 and 429, both identified as tubulin beta), which suggest different post-translational modification. Finally, 14 spots were identified as differentially expressed proteins between the BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines. Using image analysis we found that five spots (429, 896, 384, 922, 363) were up-regulated and nine spots (391, 1177, 1264, 640, 557, 703, 649, 1023, 218) were down-regulated after the silencing of MBD1 using RNAi (Fig 4).


Proteomic analysis of differential proteins in pancreatic carcinomas: Effects of MBD1 knock-down by stable RNA interference.

Liu C, Chen Y, Yu X, Jin C, Xu J, Long J, Ni Q, Fu D, Jin H, Bai C - BMC Cancer (2008)

Comparison of differentially expressed proteins between BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines. A, B, C, D, E: five areas of labeled protein spots on 2-DE gel. left panel: BxPC-3/MBD1-siRNA, right panel: BxPC-3/vector.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2386481&req=5

Figure 4: Comparison of differentially expressed proteins between BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines. A, B, C, D, E: five areas of labeled protein spots on 2-DE gel. left panel: BxPC-3/MBD1-siRNA, right panel: BxPC-3/vector.
Mentions: To investigate the changes in protein expression in pancreatic cancer after MBD1 knock-down, we performed a comparative proteomic study between BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines. Fig 3 shows two representative 2-DE maps from BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines in the pH range 4–7. Among the spots investigated, almost 30 proteins were differential expressed. But some of them were unknown proteins that could not be identified, and some of them with different PI and MW finally proved to be the same protein (such as 348 and 429, both identified as tubulin beta), which suggest different post-translational modification. Finally, 14 spots were identified as differentially expressed proteins between the BxPC-3/MBD1-siRNA and BxPC-3/vector cell lines. Using image analysis we found that five spots (429, 896, 384, 922, 363) were up-regulated and nine spots (391, 1177, 1264, 640, 557, 703, 649, 1023, 218) were down-regulated after the silencing of MBD1 using RNAi (Fig 4).

Bottom Line: Over-expression of MBD1 has been reported in human pancreatic carcinomas.We identified five proteins that were up-regulated and nine proteins that were down-regulated.Most of the identified proteins are involved in tumorigenesis, some are prognostic biomarkers for human malignant tumors.

View Article: PubMed Central - HTML - PubMed

Affiliation: Pancreatic Disease Institute, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China. blurlc@hotmail.com

ABSTRACT

Background: Methyl-CpG binding domain protein 1 (MBD1), a suppressor of gene transcription, may be involved in inactivation of tumor suppressor genes during tumorigenesis. Over-expression of MBD1 has been reported in human pancreatic carcinomas.

Methods: In this study, we established a MBD1-knock-down pancreatic cancer cell line (BxPC-3) using stable RNA interference, to compare the proteomic changes between control and MBD1-knock-down cells using two-dimensional gel electrophoresis and mass spectrometry.

Results: We identified five proteins that were up-regulated and nine proteins that were down-regulated. Most of the identified proteins are involved in tumorigenesis, some are prognostic biomarkers for human malignant tumors.

Conclusion: Our data suggest that these differential proteins may be associated with the function of MBD1, and provide some insight into the functional mechanism of MBD1 in the development of pancreatic cancer.

Show MeSH
Related in: MedlinePlus