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Food-intake-regulating-neuropeptides are expressed and regulated through pregnancy and following food restriction in rat placenta.

Caminos JE, Bravo SB, González CR, Garcés MF, Cepeda LA, González AC, Cordido F, López M, Diéguez C - Reprod. Biol. Endocrinol. (2008)

Bottom Line: Recent evidence has demonstrated their expression in rat and human placenta.Our results showed that placental-derived neuropeptides were regulated through pregnancy and following food restriction.These data could indicate that placental-derived neuropeptides represent a local regulatory circuit that may fine-tune control of energy balance during pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, School of Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain. jecaminp@gmail.com

ABSTRACT

Background: Neuropeptide Y (NPY), agouti related peptide (AgRP), cocaine and amphetamine-regulated transcript (CART) and melanocortins, the products of the proopiomelanocortin (POMC), are hypothalamic peptides involved in feeding regulation and energy homeostasis. Recent evidence has demonstrated their expression in rat and human placenta.

Methods: In the current study, we have investigated the expression of those neuropeptides in the rat placenta by real-time PCR using a model of maternal food restriction.

Results: Our results showed that placental-derived neuropeptides were regulated through pregnancy and following food restriction.

Conclusion: These data could indicate that placental-derived neuropeptides represent a local regulatory circuit that may fine-tune control of energy balance during pregnancy.

Show MeSH
NPY expression in hypothalamus and placenta of ad libitum fed and food-restricted rats. Relative NPY mRNA levels in (A) hypothalamus and (B) placenta from ad libitum fed or food-restricted female rats at different pregnancy stages (12, 16 and 21 days). Relative mRNA levels were normalized to ad libitum fed (control) as 1. **: P < 0.01 vs. ad libitum 12d; ***: P < 0.001 vs. ad libitum 12d; ###: P < 0.001 vs. ad libitum 16d; !: P < 0.05 ad libitum 21d vs. food-restricted 21d.
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Figure 1: NPY expression in hypothalamus and placenta of ad libitum fed and food-restricted rats. Relative NPY mRNA levels in (A) hypothalamus and (B) placenta from ad libitum fed or food-restricted female rats at different pregnancy stages (12, 16 and 21 days). Relative mRNA levels were normalized to ad libitum fed (control) as 1. **: P < 0.01 vs. ad libitum 12d; ***: P < 0.001 vs. ad libitum 12d; ###: P < 0.001 vs. ad libitum 16d; !: P < 0.05 ad libitum 21d vs. food-restricted 21d.

Mentions: Firstly, we studied the hypothalamic mRNA levels of NPY during pregnancy and after food restriction. Our data showed that the transcriptional expression of NPY is increased at the latest pregnancy stage (21 days; P < 0.001) with food restriction inducing a further increase at both 16 and 21 days of pregnancy (P < 0.001 and P < 0.05, respectively) (Figure 1A). Next, we evaluated the transcriptional expression of NPY in rat placenta; our results showed that placental mRNA levels of NPY were significantly higher during the initial period of gestation studied (12 days), and decreased significantly (P < 0.001) with gestational age (Figure 1B). We also studied the effect of food restriction on placental mRNA expression of NPY and these data showed that the expression profile of NPY was decreased by food restriction only at day 12 (P < 0.01), and remain unchanged between 16 to 21 days, compared to the ad libitum group (Figure 1B).


Food-intake-regulating-neuropeptides are expressed and regulated through pregnancy and following food restriction in rat placenta.

Caminos JE, Bravo SB, González CR, Garcés MF, Cepeda LA, González AC, Cordido F, López M, Diéguez C - Reprod. Biol. Endocrinol. (2008)

NPY expression in hypothalamus and placenta of ad libitum fed and food-restricted rats. Relative NPY mRNA levels in (A) hypothalamus and (B) placenta from ad libitum fed or food-restricted female rats at different pregnancy stages (12, 16 and 21 days). Relative mRNA levels were normalized to ad libitum fed (control) as 1. **: P < 0.01 vs. ad libitum 12d; ***: P < 0.001 vs. ad libitum 12d; ###: P < 0.001 vs. ad libitum 16d; !: P < 0.05 ad libitum 21d vs. food-restricted 21d.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC2386475&req=5

Figure 1: NPY expression in hypothalamus and placenta of ad libitum fed and food-restricted rats. Relative NPY mRNA levels in (A) hypothalamus and (B) placenta from ad libitum fed or food-restricted female rats at different pregnancy stages (12, 16 and 21 days). Relative mRNA levels were normalized to ad libitum fed (control) as 1. **: P < 0.01 vs. ad libitum 12d; ***: P < 0.001 vs. ad libitum 12d; ###: P < 0.001 vs. ad libitum 16d; !: P < 0.05 ad libitum 21d vs. food-restricted 21d.
Mentions: Firstly, we studied the hypothalamic mRNA levels of NPY during pregnancy and after food restriction. Our data showed that the transcriptional expression of NPY is increased at the latest pregnancy stage (21 days; P < 0.001) with food restriction inducing a further increase at both 16 and 21 days of pregnancy (P < 0.001 and P < 0.05, respectively) (Figure 1A). Next, we evaluated the transcriptional expression of NPY in rat placenta; our results showed that placental mRNA levels of NPY were significantly higher during the initial period of gestation studied (12 days), and decreased significantly (P < 0.001) with gestational age (Figure 1B). We also studied the effect of food restriction on placental mRNA expression of NPY and these data showed that the expression profile of NPY was decreased by food restriction only at day 12 (P < 0.01), and remain unchanged between 16 to 21 days, compared to the ad libitum group (Figure 1B).

Bottom Line: Recent evidence has demonstrated their expression in rat and human placenta.Our results showed that placental-derived neuropeptides were regulated through pregnancy and following food restriction.These data could indicate that placental-derived neuropeptides represent a local regulatory circuit that may fine-tune control of energy balance during pregnancy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Physiology, School of Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain. jecaminp@gmail.com

ABSTRACT

Background: Neuropeptide Y (NPY), agouti related peptide (AgRP), cocaine and amphetamine-regulated transcript (CART) and melanocortins, the products of the proopiomelanocortin (POMC), are hypothalamic peptides involved in feeding regulation and energy homeostasis. Recent evidence has demonstrated their expression in rat and human placenta.

Methods: In the current study, we have investigated the expression of those neuropeptides in the rat placenta by real-time PCR using a model of maternal food restriction.

Results: Our results showed that placental-derived neuropeptides were regulated through pregnancy and following food restriction.

Conclusion: These data could indicate that placental-derived neuropeptides represent a local regulatory circuit that may fine-tune control of energy balance during pregnancy.

Show MeSH