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Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients.

Závada J, Závadová Z, Zat'ovicová M, Hyrsl L, Kawaciuk I - Br. J. Cancer (2003)

Bottom Line: Tumour-associated protein carbonic anhydrase IX (CA IX) has two major forms.After nephrectomy, s-CA IX is cleared from the blood within a few days.Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16637 Prague, Czech Republic. zavada@img.cas.cz

ABSTRACT
Tumour-associated protein carbonic anhydrase IX (CA IX) has two major forms. One is a cell-associated, transmembrane protein seen on Western blots as a twin band of 54/58 kDa, expressed in gastric mucosa and in several types of cancer. The other is a soluble protein s-CA IX of 50/54 kDa, which is released into the culture medium or into the body fluids, most likely by proteolytic cleavage of the extracellular part from transmembrane and intracellular sequences. While TC media of CA IX-positive tumour cell lines or short-term cultures of tumour explants contain a relatively high concentration of s-CA IX (20-50 ng ml(-1)), the level of this antigen in blood serum and urine of renal clear cell carcinoma patients is about 1000 x lower. The concentration of CA IX in the blood and in urine varies within wide limits and there is no obvious correlation with tumour size. After nephrectomy, s-CA IX is cleared from the blood within a few days. Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals.

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Related in: MedlinePlus

Recovery of CA IX bands 50 and 58 kDa from diluted fluids after immunoprecipitation. A=original TC medium or extract from HT29 cells. To the same antigens as in A was added mAb M75 (ascites fluid) and Protein A-Sepharose and after appropriate incubation, the mixtures were centrifuged and analysed; B=supernatant; C=pellet. With antigens from the medium, B+C was mixed and analysed=D; with antigen from cell extract, to the pellet was added 2% FCS (=D). All samples for the analysis were adjusted to the same concentration of CA IX protein.
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fig5: Recovery of CA IX bands 50 and 58 kDa from diluted fluids after immunoprecipitation. A=original TC medium or extract from HT29 cells. To the same antigens as in A was added mAb M75 (ascites fluid) and Protein A-Sepharose and after appropriate incubation, the mixtures were centrifuged and analysed; B=supernatant; C=pellet. With antigens from the medium, B+C was mixed and analysed=D; with antigen from cell extract, to the pellet was added 2% FCS (=D). All samples for the analysis were adjusted to the same concentration of CA IX protein.

Mentions: The result was somewhat puzzling (Figure 5Figure 5


Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients.

Závada J, Závadová Z, Zat'ovicová M, Hyrsl L, Kawaciuk I - Br. J. Cancer (2003)

Recovery of CA IX bands 50 and 58 kDa from diluted fluids after immunoprecipitation. A=original TC medium or extract from HT29 cells. To the same antigens as in A was added mAb M75 (ascites fluid) and Protein A-Sepharose and after appropriate incubation, the mixtures were centrifuged and analysed; B=supernatant; C=pellet. With antigens from the medium, B+C was mixed and analysed=D; with antigen from cell extract, to the pellet was added 2% FCS (=D). All samples for the analysis were adjusted to the same concentration of CA IX protein.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376963&req=5

fig5: Recovery of CA IX bands 50 and 58 kDa from diluted fluids after immunoprecipitation. A=original TC medium or extract from HT29 cells. To the same antigens as in A was added mAb M75 (ascites fluid) and Protein A-Sepharose and after appropriate incubation, the mixtures were centrifuged and analysed; B=supernatant; C=pellet. With antigens from the medium, B+C was mixed and analysed=D; with antigen from cell extract, to the pellet was added 2% FCS (=D). All samples for the analysis were adjusted to the same concentration of CA IX protein.
Mentions: The result was somewhat puzzling (Figure 5Figure 5

Bottom Line: Tumour-associated protein carbonic anhydrase IX (CA IX) has two major forms.After nephrectomy, s-CA IX is cleared from the blood within a few days.Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16637 Prague, Czech Republic. zavada@img.cas.cz

ABSTRACT
Tumour-associated protein carbonic anhydrase IX (CA IX) has two major forms. One is a cell-associated, transmembrane protein seen on Western blots as a twin band of 54/58 kDa, expressed in gastric mucosa and in several types of cancer. The other is a soluble protein s-CA IX of 50/54 kDa, which is released into the culture medium or into the body fluids, most likely by proteolytic cleavage of the extracellular part from transmembrane and intracellular sequences. While TC media of CA IX-positive tumour cell lines or short-term cultures of tumour explants contain a relatively high concentration of s-CA IX (20-50 ng ml(-1)), the level of this antigen in blood serum and urine of renal clear cell carcinoma patients is about 1000 x lower. The concentration of CA IX in the blood and in urine varies within wide limits and there is no obvious correlation with tumour size. After nephrectomy, s-CA IX is cleared from the blood within a few days. Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals.

Show MeSH
Related in: MedlinePlus