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Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients.

Závada J, Závadová Z, Zat'ovicová M, Hyrsl L, Kawaciuk I - Br. J. Cancer (2003)

Bottom Line: Tumour-associated protein carbonic anhydrase IX (CA IX) has two major forms.After nephrectomy, s-CA IX is cleared from the blood within a few days.Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16637 Prague, Czech Republic. zavada@img.cas.cz

ABSTRACT
Tumour-associated protein carbonic anhydrase IX (CA IX) has two major forms. One is a cell-associated, transmembrane protein seen on Western blots as a twin band of 54/58 kDa, expressed in gastric mucosa and in several types of cancer. The other is a soluble protein s-CA IX of 50/54 kDa, which is released into the culture medium or into the body fluids, most likely by proteolytic cleavage of the extracellular part from transmembrane and intracellular sequences. While TC media of CA IX-positive tumour cell lines or short-term cultures of tumour explants contain a relatively high concentration of s-CA IX (20-50 ng ml(-1)), the level of this antigen in blood serum and urine of renal clear cell carcinoma patients is about 1000 x lower. The concentration of CA IX in the blood and in urine varies within wide limits and there is no obvious correlation with tumour size. After nephrectomy, s-CA IX is cleared from the blood within a few days. Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals.

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Carbonic anhydrase IX protein in the sera of RCC patients and of control individuals. Each lane was loaded with immunoprecipitate corresponding to original 0.6 ml of the serum. Lane designation: C1–C3=healthy blood donors; 100=stones in the urether; non-RCC tumours: 39=papillary carcinoma from renal cells, 103=urothelial tumour of renal pelvis, 121=sarcomatoid renal carcinoma; all other samples=RCC (the largest are no. 68=2350 cm3, no. 36=1466 cm3, no. 50=725 cm3, and the smallest no. 102=9 cm3, no. 112=14 cm3).
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fig3: Carbonic anhydrase IX protein in the sera of RCC patients and of control individuals. Each lane was loaded with immunoprecipitate corresponding to original 0.6 ml of the serum. Lane designation: C1–C3=healthy blood donors; 100=stones in the urether; non-RCC tumours: 39=papillary carcinoma from renal cells, 103=urothelial tumour of renal pelvis, 121=sarcomatoid renal carcinoma; all other samples=RCC (the largest are no. 68=2350 cm3, no. 36=1466 cm3, no. 50=725 cm3, and the smallest no. 102=9 cm3, no. 112=14 cm3).

Mentions: An example of our tests is shown in Figure 3Figure 3


Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients.

Závada J, Závadová Z, Zat'ovicová M, Hyrsl L, Kawaciuk I - Br. J. Cancer (2003)

Carbonic anhydrase IX protein in the sera of RCC patients and of control individuals. Each lane was loaded with immunoprecipitate corresponding to original 0.6 ml of the serum. Lane designation: C1–C3=healthy blood donors; 100=stones in the urether; non-RCC tumours: 39=papillary carcinoma from renal cells, 103=urothelial tumour of renal pelvis, 121=sarcomatoid renal carcinoma; all other samples=RCC (the largest are no. 68=2350 cm3, no. 36=1466 cm3, no. 50=725 cm3, and the smallest no. 102=9 cm3, no. 112=14 cm3).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376963&req=5

fig3: Carbonic anhydrase IX protein in the sera of RCC patients and of control individuals. Each lane was loaded with immunoprecipitate corresponding to original 0.6 ml of the serum. Lane designation: C1–C3=healthy blood donors; 100=stones in the urether; non-RCC tumours: 39=papillary carcinoma from renal cells, 103=urothelial tumour of renal pelvis, 121=sarcomatoid renal carcinoma; all other samples=RCC (the largest are no. 68=2350 cm3, no. 36=1466 cm3, no. 50=725 cm3, and the smallest no. 102=9 cm3, no. 112=14 cm3).
Mentions: An example of our tests is shown in Figure 3Figure 3

Bottom Line: Tumour-associated protein carbonic anhydrase IX (CA IX) has two major forms.After nephrectomy, s-CA IX is cleared from the blood within a few days.Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16637 Prague, Czech Republic. zavada@img.cas.cz

ABSTRACT
Tumour-associated protein carbonic anhydrase IX (CA IX) has two major forms. One is a cell-associated, transmembrane protein seen on Western blots as a twin band of 54/58 kDa, expressed in gastric mucosa and in several types of cancer. The other is a soluble protein s-CA IX of 50/54 kDa, which is released into the culture medium or into the body fluids, most likely by proteolytic cleavage of the extracellular part from transmembrane and intracellular sequences. While TC media of CA IX-positive tumour cell lines or short-term cultures of tumour explants contain a relatively high concentration of s-CA IX (20-50 ng ml(-1)), the level of this antigen in blood serum and urine of renal clear cell carcinoma patients is about 1000 x lower. The concentration of CA IX in the blood and in urine varies within wide limits and there is no obvious correlation with tumour size. After nephrectomy, s-CA IX is cleared from the blood within a few days. Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals.

Show MeSH
Related in: MedlinePlus