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Overexpression of hypoxia-inducible-factor 1alpha(HIF-1alpha) in oesophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage.

Kurokawa T, Miyamoto M, Kato K, Cho Y, Kawarada Y, Hida Y, Shinohara T, Itoh T, Okushiba S, Kondo S, Katoh H - Br. J. Cancer (2003)

Bottom Line: Univariate analyses identified high HIF-1alpha positivity, depth of tumour invasion, lymph node metastasis, distant metastasis, lymphatic invasion, and a positive surgical margin as risk factors.Multivariate analyses indicated that depth of tumour invasion, lymph node metastasis and positive surgical margin, but not HIF-1alpha, were independent prognostic factors.Survival in patients with a high HIF-1alpha expression was significantly worse than in those with low expression in patient treated with adjuvant therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan. t-kuro@med.hokudai.ac.jp

ABSTRACT
The purpose of this study is to investigate the clinical and histopathologic significance of hypoxia-inducible-factor 1alpha (HIF-1alpha) expression in oesophageal squamous cell carcinoma. One hundred and thirty surgically resected specimens of OSCC were immunohistochemically assessed for HIF-1alpha expression with monoclonal antibody. High HIF-1alpha immunostaining was detected in 40 specimens. The percentage of high HIF-1alpha expression cases increased with tumour stage according to pTNM system. High HIF-1alpha expression correlated with pTNM stage, depth of tumour invasion, lymph node metastasis, distant metastasis, lymphatic invasion and positive surgical margin. The overall survival rate was worse in patients with high HIF-1alpha pattern than in patients with low-expression pattern. Univariate analyses identified high HIF-1alpha positivity, depth of tumour invasion, lymph node metastasis, distant metastasis, lymphatic invasion, and a positive surgical margin as risk factors. Multivariate analyses indicated that depth of tumour invasion, lymph node metastasis and positive surgical margin, but not HIF-1alpha, were independent prognostic factors. Survival in patients with a high HIF-1alpha expression was significantly worse than in those with low expression in patient treated with adjuvant therapy.

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Representative photomicrographs of immunohistochemical staining of HIF1α (× 200). Tumour cell immunoreactivity was scored based on nuclear and cytoplasmic staining. (A) −, no staining (B) 1+, nuclear staining in less than 1% of cells (C) nuclear staining in 1-10% of cells and/or with weak cytoplasmic staining (D) 3+, nuclear staining in 10-50% of cells and/or with distinct cytoplasmic staining, (E) 4+, nuclear staining in more than 50% of cells and/or with strong cytoplasmic staining. (F) HIF-1α-positive cells are already found in carcinoma in situ.
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fig1: Representative photomicrographs of immunohistochemical staining of HIF1α (× 200). Tumour cell immunoreactivity was scored based on nuclear and cytoplasmic staining. (A) −, no staining (B) 1+, nuclear staining in less than 1% of cells (C) nuclear staining in 1-10% of cells and/or with weak cytoplasmic staining (D) 3+, nuclear staining in 10-50% of cells and/or with distinct cytoplasmic staining, (E) 4+, nuclear staining in more than 50% of cells and/or with strong cytoplasmic staining. (F) HIF-1α-positive cells are already found in carcinoma in situ.

Mentions: A total of 130 OSCCs were grouped as 42 HIF-1α negative tumours; 15 HIF-1α 1+tumours; 33 HIF-1α 2+tumours; 30 HIF-1α 3+tumours; and 10 HIF-1α 4+tumours (Figure 1Figure 1


Overexpression of hypoxia-inducible-factor 1alpha(HIF-1alpha) in oesophageal squamous cell carcinoma correlates with lymph node metastasis and pathologic stage.

Kurokawa T, Miyamoto M, Kato K, Cho Y, Kawarada Y, Hida Y, Shinohara T, Itoh T, Okushiba S, Kondo S, Katoh H - Br. J. Cancer (2003)

Representative photomicrographs of immunohistochemical staining of HIF1α (× 200). Tumour cell immunoreactivity was scored based on nuclear and cytoplasmic staining. (A) −, no staining (B) 1+, nuclear staining in less than 1% of cells (C) nuclear staining in 1-10% of cells and/or with weak cytoplasmic staining (D) 3+, nuclear staining in 10-50% of cells and/or with distinct cytoplasmic staining, (E) 4+, nuclear staining in more than 50% of cells and/or with strong cytoplasmic staining. (F) HIF-1α-positive cells are already found in carcinoma in situ.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376949&req=5

fig1: Representative photomicrographs of immunohistochemical staining of HIF1α (× 200). Tumour cell immunoreactivity was scored based on nuclear and cytoplasmic staining. (A) −, no staining (B) 1+, nuclear staining in less than 1% of cells (C) nuclear staining in 1-10% of cells and/or with weak cytoplasmic staining (D) 3+, nuclear staining in 10-50% of cells and/or with distinct cytoplasmic staining, (E) 4+, nuclear staining in more than 50% of cells and/or with strong cytoplasmic staining. (F) HIF-1α-positive cells are already found in carcinoma in situ.
Mentions: A total of 130 OSCCs were grouped as 42 HIF-1α negative tumours; 15 HIF-1α 1+tumours; 33 HIF-1α 2+tumours; 30 HIF-1α 3+tumours; and 10 HIF-1α 4+tumours (Figure 1Figure 1

Bottom Line: Univariate analyses identified high HIF-1alpha positivity, depth of tumour invasion, lymph node metastasis, distant metastasis, lymphatic invasion, and a positive surgical margin as risk factors.Multivariate analyses indicated that depth of tumour invasion, lymph node metastasis and positive surgical margin, but not HIF-1alpha, were independent prognostic factors.Survival in patients with a high HIF-1alpha expression was significantly worse than in those with low expression in patient treated with adjuvant therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan. t-kuro@med.hokudai.ac.jp

ABSTRACT
The purpose of this study is to investigate the clinical and histopathologic significance of hypoxia-inducible-factor 1alpha (HIF-1alpha) expression in oesophageal squamous cell carcinoma. One hundred and thirty surgically resected specimens of OSCC were immunohistochemically assessed for HIF-1alpha expression with monoclonal antibody. High HIF-1alpha immunostaining was detected in 40 specimens. The percentage of high HIF-1alpha expression cases increased with tumour stage according to pTNM system. High HIF-1alpha expression correlated with pTNM stage, depth of tumour invasion, lymph node metastasis, distant metastasis, lymphatic invasion and positive surgical margin. The overall survival rate was worse in patients with high HIF-1alpha pattern than in patients with low-expression pattern. Univariate analyses identified high HIF-1alpha positivity, depth of tumour invasion, lymph node metastasis, distant metastasis, lymphatic invasion, and a positive surgical margin as risk factors. Multivariate analyses indicated that depth of tumour invasion, lymph node metastasis and positive surgical margin, but not HIF-1alpha, were independent prognostic factors. Survival in patients with a high HIF-1alpha expression was significantly worse than in those with low expression in patient treated with adjuvant therapy.

Show MeSH
Related in: MedlinePlus