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Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions.

Chatrath P, Scott IS, Morris LS, Davies RJ, Rushbrook SM, Bird K, Vowler SL, Grant JW, Saeed IT, Howard D, Laskey RA, Coleman N - Br. J. Cancer (2003)

Bottom Line: Expression patterns were compared using double labelling confocal microscopy.Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions.Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.

View Article: PubMed Central - PubMed

Affiliation: MRC Cancer Cell Unit, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK.

ABSTRACT
Histological classification of laryngeal epithelial lesions is highly subjective, and methods of cytological detection are not well developed. Improved determination of aberrant cell cycle entry may allow increased objectivity in histological assessment and enable the development of less invasive diagnostic cytology tests. Sections of normal larynx (n=10), laryngeal dysplasia (n=20) and laryngeal squamous cell carcinoma (SCC) (n=10) were classified according to the Ljubljana classification and stained for markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67. Expression patterns were compared using double labelling confocal microscopy. There was a correlation between Mcm-2 and Ki67 labelling indices (rho=0.93; 95% CI [0.84, 0.97]) and both markers showed increased expression from normal epithelium to SCC (Mcm-2, P=0.001; Ki67, P=0.0002). Importantly, there was minimal expression of Mcm-2 or Ki67 in the most superficial layers of normal larynx and abnormal or atypical hyperplasia, in contrast to carcinoma in situ and SCC. Clusters of Mcm-2/5-positive cells were present in cytological preparations from SCC, but not from those showing atypical hyperplasia or inflammation in non-neoplastic tissue. Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions. Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.

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Normal squamous (A) and respiratory-type (B) epithelial cells are negative when stained with antibodies against Mcm-2/5. (C) A cluster of cells from a laryngeal SCC, which are strongly positive for Mcm-2/5 and were readily identified, even at low magnification.
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fig6: Normal squamous (A) and respiratory-type (B) epithelial cells are negative when stained with antibodies against Mcm-2/5. (C) A cluster of cells from a laryngeal SCC, which are strongly positive for Mcm-2/5 and were readily identified, even at low magnification.

Mentions: In view of the high frequency of expression of Mcm-2 in the superficial third of dysplastic epithelia (including in sloughed cells), we undertook a small pilot study to investigate whether laryngeal lesions could be detected by immunocytochemical analysis of MCM proteins in laryngeal brushings. Brushings were obtained from lesions (n=6) from which a biopsy was also taken as a gold standard (Figure 6Figure 6


Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions.

Chatrath P, Scott IS, Morris LS, Davies RJ, Rushbrook SM, Bird K, Vowler SL, Grant JW, Saeed IT, Howard D, Laskey RA, Coleman N - Br. J. Cancer (2003)

Normal squamous (A) and respiratory-type (B) epithelial cells are negative when stained with antibodies against Mcm-2/5. (C) A cluster of cells from a laryngeal SCC, which are strongly positive for Mcm-2/5 and were readily identified, even at low magnification.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376943&req=5

fig6: Normal squamous (A) and respiratory-type (B) epithelial cells are negative when stained with antibodies against Mcm-2/5. (C) A cluster of cells from a laryngeal SCC, which are strongly positive for Mcm-2/5 and were readily identified, even at low magnification.
Mentions: In view of the high frequency of expression of Mcm-2 in the superficial third of dysplastic epithelia (including in sloughed cells), we undertook a small pilot study to investigate whether laryngeal lesions could be detected by immunocytochemical analysis of MCM proteins in laryngeal brushings. Brushings were obtained from lesions (n=6) from which a biopsy was also taken as a gold standard (Figure 6Figure 6

Bottom Line: Expression patterns were compared using double labelling confocal microscopy.Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions.Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.

View Article: PubMed Central - PubMed

Affiliation: MRC Cancer Cell Unit, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK.

ABSTRACT
Histological classification of laryngeal epithelial lesions is highly subjective, and methods of cytological detection are not well developed. Improved determination of aberrant cell cycle entry may allow increased objectivity in histological assessment and enable the development of less invasive diagnostic cytology tests. Sections of normal larynx (n=10), laryngeal dysplasia (n=20) and laryngeal squamous cell carcinoma (SCC) (n=10) were classified according to the Ljubljana classification and stained for markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67. Expression patterns were compared using double labelling confocal microscopy. There was a correlation between Mcm-2 and Ki67 labelling indices (rho=0.93; 95% CI [0.84, 0.97]) and both markers showed increased expression from normal epithelium to SCC (Mcm-2, P=0.001; Ki67, P=0.0002). Importantly, there was minimal expression of Mcm-2 or Ki67 in the most superficial layers of normal larynx and abnormal or atypical hyperplasia, in contrast to carcinoma in situ and SCC. Clusters of Mcm-2/5-positive cells were present in cytological preparations from SCC, but not from those showing atypical hyperplasia or inflammation in non-neoplastic tissue. Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions. Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.

Show MeSH
Related in: MedlinePlus