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Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions.

Chatrath P, Scott IS, Morris LS, Davies RJ, Rushbrook SM, Bird K, Vowler SL, Grant JW, Saeed IT, Howard D, Laskey RA, Coleman N - Br. J. Cancer (2003)

Bottom Line: Expression patterns were compared using double labelling confocal microscopy.Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions.Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.

View Article: PubMed Central - PubMed

Affiliation: MRC Cancer Cell Unit, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK.

ABSTRACT
Histological classification of laryngeal epithelial lesions is highly subjective, and methods of cytological detection are not well developed. Improved determination of aberrant cell cycle entry may allow increased objectivity in histological assessment and enable the development of less invasive diagnostic cytology tests. Sections of normal larynx (n=10), laryngeal dysplasia (n=20) and laryngeal squamous cell carcinoma (SCC) (n=10) were classified according to the Ljubljana classification and stained for markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67. Expression patterns were compared using double labelling confocal microscopy. There was a correlation between Mcm-2 and Ki67 labelling indices (rho=0.93; 95% CI [0.84, 0.97]) and both markers showed increased expression from normal epithelium to SCC (Mcm-2, P=0.001; Ki67, P=0.0002). Importantly, there was minimal expression of Mcm-2 or Ki67 in the most superficial layers of normal larynx and abnormal or atypical hyperplasia, in contrast to carcinoma in situ and SCC. Clusters of Mcm-2/5-positive cells were present in cytological preparations from SCC, but not from those showing atypical hyperplasia or inflammation in non-neoplastic tissue. Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions. Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.

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Bar charts (median±IQR) comparing Mcm-2 LIs in different compartments of the laryngeal epithelium in normal larynx (n=8), atypical hyperplasia (n=10), carcinoma in situ (n=9). Substantial expression of Mcm-2 in the superficial epithelial third is only present in carcinoma in situ, although some increase in expression above normal is seen in atypical hyperplasia. Base=lower epithelial third, asterisk and circle=extreme values.
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fig4: Bar charts (median±IQR) comparing Mcm-2 LIs in different compartments of the laryngeal epithelium in normal larynx (n=8), atypical hyperplasia (n=10), carcinoma in situ (n=9). Substantial expression of Mcm-2 in the superficial epithelial third is only present in carcinoma in situ, although some increase in expression above normal is seen in atypical hyperplasia. Base=lower epithelial third, asterisk and circle=extreme values.

Mentions: Mcm-2 LIs were significantly greater in lesions showing dysplasia (atypical hyperplasia and carcinoma in situ combined) in comparison to normal larynx in the superficial (P<0.0001), middle (P<0.0001) and lower thirds of the epithelium (P=0.003) and in the basal layer (P<0.0001) (Figure 2A). Substantial expression of Mcm-2 in the superficial third was only seen in carcinoma in situ (WHO: LD-3, CIS), although some increase in expression was seen in atypical hyperplasia (WHO: LD-1/2) (Figure 4Figure 4


Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions.

Chatrath P, Scott IS, Morris LS, Davies RJ, Rushbrook SM, Bird K, Vowler SL, Grant JW, Saeed IT, Howard D, Laskey RA, Coleman N - Br. J. Cancer (2003)

Bar charts (median±IQR) comparing Mcm-2 LIs in different compartments of the laryngeal epithelium in normal larynx (n=8), atypical hyperplasia (n=10), carcinoma in situ (n=9). Substantial expression of Mcm-2 in the superficial epithelial third is only present in carcinoma in situ, although some increase in expression above normal is seen in atypical hyperplasia. Base=lower epithelial third, asterisk and circle=extreme values.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376943&req=5

fig4: Bar charts (median±IQR) comparing Mcm-2 LIs in different compartments of the laryngeal epithelium in normal larynx (n=8), atypical hyperplasia (n=10), carcinoma in situ (n=9). Substantial expression of Mcm-2 in the superficial epithelial third is only present in carcinoma in situ, although some increase in expression above normal is seen in atypical hyperplasia. Base=lower epithelial third, asterisk and circle=extreme values.
Mentions: Mcm-2 LIs were significantly greater in lesions showing dysplasia (atypical hyperplasia and carcinoma in situ combined) in comparison to normal larynx in the superficial (P<0.0001), middle (P<0.0001) and lower thirds of the epithelium (P=0.003) and in the basal layer (P<0.0001) (Figure 2A). Substantial expression of Mcm-2 in the superficial third was only seen in carcinoma in situ (WHO: LD-3, CIS), although some increase in expression was seen in atypical hyperplasia (WHO: LD-1/2) (Figure 4Figure 4

Bottom Line: Expression patterns were compared using double labelling confocal microscopy.Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions.Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.

View Article: PubMed Central - PubMed

Affiliation: MRC Cancer Cell Unit, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK.

ABSTRACT
Histological classification of laryngeal epithelial lesions is highly subjective, and methods of cytological detection are not well developed. Improved determination of aberrant cell cycle entry may allow increased objectivity in histological assessment and enable the development of less invasive diagnostic cytology tests. Sections of normal larynx (n=10), laryngeal dysplasia (n=20) and laryngeal squamous cell carcinoma (SCC) (n=10) were classified according to the Ljubljana classification and stained for markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67. Expression patterns were compared using double labelling confocal microscopy. There was a correlation between Mcm-2 and Ki67 labelling indices (rho=0.93; 95% CI [0.84, 0.97]) and both markers showed increased expression from normal epithelium to SCC (Mcm-2, P=0.001; Ki67, P=0.0002). Importantly, there was minimal expression of Mcm-2 or Ki67 in the most superficial layers of normal larynx and abnormal or atypical hyperplasia, in contrast to carcinoma in situ and SCC. Clusters of Mcm-2/5-positive cells were present in cytological preparations from SCC, but not from those showing atypical hyperplasia or inflammation in non-neoplastic tissue. Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions. Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.

Show MeSH
Related in: MedlinePlus