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n-3 polyunsaturated fatty acids decrease mucosal/epidermal reactions and enhance antitumour effect of ionising radiation with inhibition of tumour angiogenesis.

Wen B, Deutsch E, Opolon P, Auperin A, Frascogna V, Connault E, Bourhis J - Br. J. Cancer (2003)

Bottom Line: The effect of n-3 PUFAs was associated with inhibition of angiogenesis and tumour proliferation, and significantly decreased expression of cyclooxygenase-2.In conclusion, n-3 PUFAs administration decrease mucosal response, while moderately enhancing the antitumour effect of irradiation.The magnitude of the differential effect suggests that n-3 PUFAs need to be further investigated in the clinic.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire UPRES EA No. 27-10 Radiosensibilité des tumeurs et tissus sains, Institut Gustave-Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cédex, France.

ABSTRACT
The purpose of this study was to evaluate the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs) on normal tissue (lip mucosa) and tumour growth when combined with ionising radiation. The oral region (snout) of C57 black mice was irradiated with 16.5 Gy and n-3 PUFAs (100 microl) were injected intravenously for 2 weeks. After exposure to irradiation, the degree and duration of the acute reactions decreased significantly when mice were treated with n-3 PUFAs as compared to the control group. Interestingly, the range of the reactions in the n-3 PUFAs-treated group compared favourably to the group receiving amifostine (27.5 mg/kg i.v.). the effect of n-3 PUFAs was further evaluated in HEP-2 human carcinoma xenograft transplanted in nude mice. An inhibition of tumour growth was observed when mice were treated with n-3 PUFAs alone and this effect was maximal when combined with irradiation. Similar results were obtained using eicosapentaenoic acid. The effect of n-3 PUFAs was associated with inhibition of angiogenesis and tumour proliferation, and significantly decreased expression of cyclooxygenase-2. In conclusion, n-3 PUFAs administration decrease mucosal response, while moderately enhancing the antitumour effect of irradiation. The magnitude of the differential effect suggests that n-3 PUFAs need to be further investigated in the clinic.

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Detection of vessels within the tumour of HEP-2 tumour xenograft: Pecam-1 endothelial immunostaining, magnification × 100. (A) Control group, highly vascularised tumour with thick and large vascular channels. Presence of large vessels located at the periphery of the tumour. (B) Irradiation alone, moderately vascularised tumours. (C) n-3 PUFA alone, moderately vascularised tumours. (D) Irradiation combined with n-3 PUFA, marked inhibition in tumour vascularisation.
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fig5: Detection of vessels within the tumour of HEP-2 tumour xenograft: Pecam-1 endothelial immunostaining, magnification × 100. (A) Control group, highly vascularised tumour with thick and large vascular channels. Presence of large vessels located at the periphery of the tumour. (B) Irradiation alone, moderately vascularised tumours. (C) n-3 PUFA alone, moderately vascularised tumours. (D) Irradiation combined with n-3 PUFA, marked inhibition in tumour vascularisation.

Mentions: The histological examination of the tumours showed a typical viable squamous cell carcinoma with various degrees of necrosis in the control group, fewer focal areas of viable cells surrounded by fibrocytes in the n-3 PUFAs group and almost undetectable tumour cells in the group of n-3 PUFAs plus irradiation (Figure 5Figure 5


n-3 polyunsaturated fatty acids decrease mucosal/epidermal reactions and enhance antitumour effect of ionising radiation with inhibition of tumour angiogenesis.

Wen B, Deutsch E, Opolon P, Auperin A, Frascogna V, Connault E, Bourhis J - Br. J. Cancer (2003)

Detection of vessels within the tumour of HEP-2 tumour xenograft: Pecam-1 endothelial immunostaining, magnification × 100. (A) Control group, highly vascularised tumour with thick and large vascular channels. Presence of large vessels located at the periphery of the tumour. (B) Irradiation alone, moderately vascularised tumours. (C) n-3 PUFA alone, moderately vascularised tumours. (D) Irradiation combined with n-3 PUFA, marked inhibition in tumour vascularisation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376938&req=5

fig5: Detection of vessels within the tumour of HEP-2 tumour xenograft: Pecam-1 endothelial immunostaining, magnification × 100. (A) Control group, highly vascularised tumour with thick and large vascular channels. Presence of large vessels located at the periphery of the tumour. (B) Irradiation alone, moderately vascularised tumours. (C) n-3 PUFA alone, moderately vascularised tumours. (D) Irradiation combined with n-3 PUFA, marked inhibition in tumour vascularisation.
Mentions: The histological examination of the tumours showed a typical viable squamous cell carcinoma with various degrees of necrosis in the control group, fewer focal areas of viable cells surrounded by fibrocytes in the n-3 PUFAs group and almost undetectable tumour cells in the group of n-3 PUFAs plus irradiation (Figure 5Figure 5

Bottom Line: The effect of n-3 PUFAs was associated with inhibition of angiogenesis and tumour proliferation, and significantly decreased expression of cyclooxygenase-2.In conclusion, n-3 PUFAs administration decrease mucosal response, while moderately enhancing the antitumour effect of irradiation.The magnitude of the differential effect suggests that n-3 PUFAs need to be further investigated in the clinic.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire UPRES EA No. 27-10 Radiosensibilité des tumeurs et tissus sains, Institut Gustave-Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cédex, France.

ABSTRACT
The purpose of this study was to evaluate the effect of n-3 polyunsaturated fatty acids (n-3 PUFAs) on normal tissue (lip mucosa) and tumour growth when combined with ionising radiation. The oral region (snout) of C57 black mice was irradiated with 16.5 Gy and n-3 PUFAs (100 microl) were injected intravenously for 2 weeks. After exposure to irradiation, the degree and duration of the acute reactions decreased significantly when mice were treated with n-3 PUFAs as compared to the control group. Interestingly, the range of the reactions in the n-3 PUFAs-treated group compared favourably to the group receiving amifostine (27.5 mg/kg i.v.). the effect of n-3 PUFAs was further evaluated in HEP-2 human carcinoma xenograft transplanted in nude mice. An inhibition of tumour growth was observed when mice were treated with n-3 PUFAs alone and this effect was maximal when combined with irradiation. Similar results were obtained using eicosapentaenoic acid. The effect of n-3 PUFAs was associated with inhibition of angiogenesis and tumour proliferation, and significantly decreased expression of cyclooxygenase-2. In conclusion, n-3 PUFAs administration decrease mucosal response, while moderately enhancing the antitumour effect of irradiation. The magnitude of the differential effect suggests that n-3 PUFAs need to be further investigated in the clinic.

Show MeSH
Related in: MedlinePlus