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Selective accumulation of ALA-induced PpIX and photodynamic effect in chemically induced hepatocellular carcinoma.

Otake M, Nishiwaki M, Kobayashi Y, Baba S, Kohno E, Kawasaki T, Fujise Y, Nakamura H - Br. J. Cancer (2003)

Bottom Line: Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for PDT.Based on these results, PDT was performed on HCC at 3 h after 500 mg x kg(-1) ALA administration before laser irradiation of 30 J per tumour.Antitumour effect was more evident in HCC than in the nontumoral tissue surrounding HCC.

View Article: PubMed Central - PubMed

Affiliation: Second Department of Internal Medicine, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, Japan. mamikoo@dream.com

ABSTRACT
The possibility of 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) for liver cancer was investigated using a chemically induced hepatocellular carcinoma (HCC) model. Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for PDT. We determined the fluorescence intensity of PpIX in HCC and nontumoral tissue in the liver. 5-Aminolaevulinic acid was intravenously injected to male Fisher rats with HCC at a dose of 500 mg x kg(-1), and the fluorescence intensity in each tissue sample excised from liver was measured with a spectrofluorometer at 1, 3 and 6 h after administration. Fluorescence intensity was at a peak of 3 h after administration in both HCC and nontumoral tissue. The accumulation of PpIX in HCC was higher than that in the nontumoral tissue at 1 h (P<0.001) and 3 h (P<0.05) after ALA administration. Based on these results, PDT was performed on HCC at 3 h after 500 mg x kg(-1) ALA administration before laser irradiation of 30 J per tumour. Antitumour effect was more evident in HCC than in the nontumoral tissue surrounding HCC. These findings suggest the possibility to detect HCC by fluorescence and to treat HCC by light.

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(A) Fluorescence micrograph of the liver including HCC at 3 h after 500 mg kg−1 ALA administration. The right part of the specimen was illuminated reddish-orange by excitation of blue light. (B) The specimen stained with HE. Histopathological examination revealed the right part to be HCC and the left part to be non-HCC. Scale: a bar represents 0.1 mm.
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fig6: (A) Fluorescence micrograph of the liver including HCC at 3 h after 500 mg kg−1 ALA administration. The right part of the specimen was illuminated reddish-orange by excitation of blue light. (B) The specimen stained with HE. Histopathological examination revealed the right part to be HCC and the left part to be non-HCC. Scale: a bar represents 0.1 mm.

Mentions: (A) Fluorescence photograph of the liver including HCC at 3 h after 500 mg kg−1 ALA administration. The tumour at the edge of the liver (arrow) was illuminated by excitation of blue light. (B) The specimen stained with HE. The tumour was revealed to be HCC. Scale: a bar represents 0.1 mm.


Selective accumulation of ALA-induced PpIX and photodynamic effect in chemically induced hepatocellular carcinoma.

Otake M, Nishiwaki M, Kobayashi Y, Baba S, Kohno E, Kawasaki T, Fujise Y, Nakamura H - Br. J. Cancer (2003)

(A) Fluorescence micrograph of the liver including HCC at 3 h after 500 mg kg−1 ALA administration. The right part of the specimen was illuminated reddish-orange by excitation of blue light. (B) The specimen stained with HE. Histopathological examination revealed the right part to be HCC and the left part to be non-HCC. Scale: a bar represents 0.1 mm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376916&req=5

fig6: (A) Fluorescence micrograph of the liver including HCC at 3 h after 500 mg kg−1 ALA administration. The right part of the specimen was illuminated reddish-orange by excitation of blue light. (B) The specimen stained with HE. Histopathological examination revealed the right part to be HCC and the left part to be non-HCC. Scale: a bar represents 0.1 mm.
Mentions: (A) Fluorescence photograph of the liver including HCC at 3 h after 500 mg kg−1 ALA administration. The tumour at the edge of the liver (arrow) was illuminated by excitation of blue light. (B) The specimen stained with HE. The tumour was revealed to be HCC. Scale: a bar represents 0.1 mm.

Bottom Line: Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for PDT.Based on these results, PDT was performed on HCC at 3 h after 500 mg x kg(-1) ALA administration before laser irradiation of 30 J per tumour.Antitumour effect was more evident in HCC than in the nontumoral tissue surrounding HCC.

View Article: PubMed Central - PubMed

Affiliation: Second Department of Internal Medicine, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, Japan. mamikoo@dream.com

ABSTRACT
The possibility of 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) for liver cancer was investigated using a chemically induced hepatocellular carcinoma (HCC) model. Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for PDT. We determined the fluorescence intensity of PpIX in HCC and nontumoral tissue in the liver. 5-Aminolaevulinic acid was intravenously injected to male Fisher rats with HCC at a dose of 500 mg x kg(-1), and the fluorescence intensity in each tissue sample excised from liver was measured with a spectrofluorometer at 1, 3 and 6 h after administration. Fluorescence intensity was at a peak of 3 h after administration in both HCC and nontumoral tissue. The accumulation of PpIX in HCC was higher than that in the nontumoral tissue at 1 h (P<0.001) and 3 h (P<0.05) after ALA administration. Based on these results, PDT was performed on HCC at 3 h after 500 mg x kg(-1) ALA administration before laser irradiation of 30 J per tumour. Antitumour effect was more evident in HCC than in the nontumoral tissue surrounding HCC. These findings suggest the possibility to detect HCC by fluorescence and to treat HCC by light.

Show MeSH
Related in: MedlinePlus