Limits...
Selective accumulation of ALA-induced PpIX and photodynamic effect in chemically induced hepatocellular carcinoma.

Otake M, Nishiwaki M, Kobayashi Y, Baba S, Kohno E, Kawasaki T, Fujise Y, Nakamura H - Br. J. Cancer (2003)

Bottom Line: Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for PDT.Based on these results, PDT was performed on HCC at 3 h after 500 mg x kg(-1) ALA administration before laser irradiation of 30 J per tumour.Antitumour effect was more evident in HCC than in the nontumoral tissue surrounding HCC.

View Article: PubMed Central - PubMed

Affiliation: Second Department of Internal Medicine, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, Japan. mamikoo@dream.com

ABSTRACT
The possibility of 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) for liver cancer was investigated using a chemically induced hepatocellular carcinoma (HCC) model. Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for PDT. We determined the fluorescence intensity of PpIX in HCC and nontumoral tissue in the liver. 5-Aminolaevulinic acid was intravenously injected to male Fisher rats with HCC at a dose of 500 mg x kg(-1), and the fluorescence intensity in each tissue sample excised from liver was measured with a spectrofluorometer at 1, 3 and 6 h after administration. Fluorescence intensity was at a peak of 3 h after administration in both HCC and nontumoral tissue. The accumulation of PpIX in HCC was higher than that in the nontumoral tissue at 1 h (P<0.001) and 3 h (P<0.05) after ALA administration. Based on these results, PDT was performed on HCC at 3 h after 500 mg x kg(-1) ALA administration before laser irradiation of 30 J per tumour. Antitumour effect was more evident in HCC than in the nontumoral tissue surrounding HCC. These findings suggest the possibility to detect HCC by fluorescence and to treat HCC by light.

Show MeSH

Related in: MedlinePlus

Fluorescence intensity of PpIX in HCC and in nontumoral tissue at various time intervals after intravenous administration of 500 mg kg−1 ALA. Bars (s.e.) are based on between eight and 19 tissues. ▪ HCC,  nontumoral tissue. Significant difference: *P<0.01.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC2376916&req=5

fig3: Fluorescence intensity of PpIX in HCC and in nontumoral tissue at various time intervals after intravenous administration of 500 mg kg−1 ALA. Bars (s.e.) are based on between eight and 19 tissues. ▪ HCC, nontumoral tissue. Significant difference: *P<0.01.

Mentions: Fluorescence intensity of PpIX in HCC and in nontumoral tissue was compared. The measurements are 16 HCCs and 15 nontumoral tissues at 1 h, 10 HCCs and 19 nontumoral tissues at 3 h, eight HCCs and 13 nontumoral tissues at 6 h. Fluorescence intensity in HCC and nontumoral tissue at various time intervals after 500 mg kg−1 ALA accumulation is shown in Figure 3Figure 3


Selective accumulation of ALA-induced PpIX and photodynamic effect in chemically induced hepatocellular carcinoma.

Otake M, Nishiwaki M, Kobayashi Y, Baba S, Kohno E, Kawasaki T, Fujise Y, Nakamura H - Br. J. Cancer (2003)

Fluorescence intensity of PpIX in HCC and in nontumoral tissue at various time intervals after intravenous administration of 500 mg kg−1 ALA. Bars (s.e.) are based on between eight and 19 tissues. ▪ HCC,  nontumoral tissue. Significant difference: *P<0.01.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376916&req=5

fig3: Fluorescence intensity of PpIX in HCC and in nontumoral tissue at various time intervals after intravenous administration of 500 mg kg−1 ALA. Bars (s.e.) are based on between eight and 19 tissues. ▪ HCC, nontumoral tissue. Significant difference: *P<0.01.
Mentions: Fluorescence intensity of PpIX in HCC and in nontumoral tissue was compared. The measurements are 16 HCCs and 15 nontumoral tissues at 1 h, 10 HCCs and 19 nontumoral tissues at 3 h, eight HCCs and 13 nontumoral tissues at 6 h. Fluorescence intensity in HCC and nontumoral tissue at various time intervals after 500 mg kg−1 ALA accumulation is shown in Figure 3Figure 3

Bottom Line: Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for PDT.Based on these results, PDT was performed on HCC at 3 h after 500 mg x kg(-1) ALA administration before laser irradiation of 30 J per tumour.Antitumour effect was more evident in HCC than in the nontumoral tissue surrounding HCC.

View Article: PubMed Central - PubMed

Affiliation: Second Department of Internal Medicine, Hamamatsu University School of Medicine, Handayama 1-20-1, Hamamatsu, Shizuoka 431-3192, Japan. mamikoo@dream.com

ABSTRACT
The possibility of 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) for liver cancer was investigated using a chemically induced hepatocellular carcinoma (HCC) model. Endogenously synthesised protoporphyrin IX (PpIX) following the administration of ALA is an effective photosensitiser for PDT. We determined the fluorescence intensity of PpIX in HCC and nontumoral tissue in the liver. 5-Aminolaevulinic acid was intravenously injected to male Fisher rats with HCC at a dose of 500 mg x kg(-1), and the fluorescence intensity in each tissue sample excised from liver was measured with a spectrofluorometer at 1, 3 and 6 h after administration. Fluorescence intensity was at a peak of 3 h after administration in both HCC and nontumoral tissue. The accumulation of PpIX in HCC was higher than that in the nontumoral tissue at 1 h (P<0.001) and 3 h (P<0.05) after ALA administration. Based on these results, PDT was performed on HCC at 3 h after 500 mg x kg(-1) ALA administration before laser irradiation of 30 J per tumour. Antitumour effect was more evident in HCC than in the nontumoral tissue surrounding HCC. These findings suggest the possibility to detect HCC by fluorescence and to treat HCC by light.

Show MeSH
Related in: MedlinePlus