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Micronuclei to detect in vivo chemotherapy damage in a p53 mutated solid tumour.

Driessens G, Harsan L, Robaye B, Waroquier D, Browaeys P, Giannakopoulos X, Velu T, Bruyns C - Br. J. Cancer (2003)

Bottom Line: Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity.In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status.In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Research Institute (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles, 1070 Brussels, Belgium.

ABSTRACT
Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity. In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status. In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.

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In vivo induction of apoptosis or MN formation in 9L tumours after local γ-irradiation or systemic injection of cisplatin. TUNEL assay on tumour section from control nontreated rat (A), cisplatin (1 mg/kg−1) treated rat (B) or locally γ-irradiated rat (C). Total percentages of binucleated (BN) cells with MN (D) in 9L tumours. Percentages of BN cells with MN divided into subcategories depending on the number of MN per cell (E). *Significantly different from the control (P<0.05). Magnification bars=0.1 mm.
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fig2: In vivo induction of apoptosis or MN formation in 9L tumours after local γ-irradiation or systemic injection of cisplatin. TUNEL assay on tumour section from control nontreated rat (A), cisplatin (1 mg/kg−1) treated rat (B) or locally γ-irradiated rat (C). Total percentages of binucleated (BN) cells with MN (D) in 9L tumours. Percentages of BN cells with MN divided into subcategories depending on the number of MN per cell (E). *Significantly different from the control (P<0.05). Magnification bars=0.1 mm.

Mentions: In vivo induction of apoptosis in established 9L tumours was assessed after local tumour irradiation (20 Gy) or systemic injection of a nontoxic dose of cisplatin (1 mg kg−1) (Burger et al, 2002). Data from TUNEL assays (Figure 2A–CFigure 2


Micronuclei to detect in vivo chemotherapy damage in a p53 mutated solid tumour.

Driessens G, Harsan L, Robaye B, Waroquier D, Browaeys P, Giannakopoulos X, Velu T, Bruyns C - Br. J. Cancer (2003)

In vivo induction of apoptosis or MN formation in 9L tumours after local γ-irradiation or systemic injection of cisplatin. TUNEL assay on tumour section from control nontreated rat (A), cisplatin (1 mg/kg−1) treated rat (B) or locally γ-irradiated rat (C). Total percentages of binucleated (BN) cells with MN (D) in 9L tumours. Percentages of BN cells with MN divided into subcategories depending on the number of MN per cell (E). *Significantly different from the control (P<0.05). Magnification bars=0.1 mm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376913&req=5

fig2: In vivo induction of apoptosis or MN formation in 9L tumours after local γ-irradiation or systemic injection of cisplatin. TUNEL assay on tumour section from control nontreated rat (A), cisplatin (1 mg/kg−1) treated rat (B) or locally γ-irradiated rat (C). Total percentages of binucleated (BN) cells with MN (D) in 9L tumours. Percentages of BN cells with MN divided into subcategories depending on the number of MN per cell (E). *Significantly different from the control (P<0.05). Magnification bars=0.1 mm.
Mentions: In vivo induction of apoptosis in established 9L tumours was assessed after local tumour irradiation (20 Gy) or systemic injection of a nontoxic dose of cisplatin (1 mg kg−1) (Burger et al, 2002). Data from TUNEL assays (Figure 2A–CFigure 2

Bottom Line: Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity.In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status.In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Research Institute (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles, 1070 Brussels, Belgium.

ABSTRACT
Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity. In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status. In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.

Show MeSH
Related in: MedlinePlus