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Micronuclei to detect in vivo chemotherapy damage in a p53 mutated solid tumour.

Driessens G, Harsan L, Robaye B, Waroquier D, Browaeys P, Giannakopoulos X, Velu T, Bruyns C - Br. J. Cancer (2003)

Bottom Line: Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity.In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status.In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Research Institute (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles, 1070 Brussels, Belgium.

ABSTRACT
Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity. In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status. In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.

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In vitro induction of apoptosis or MN formation after chemo- or radiotherapy. Measure of caspase-3 activity in 9L (A) and C6 (C) cells. Measure of PS externalisation by AnnexinV–FITC binding assay on 9L (B) and C6 cells (D). Total percentages of 9L (E) and C6 (F) binucleated cells with MN. Significantly different from the control: *P<0.05, **P<0.01.
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fig1: In vitro induction of apoptosis or MN formation after chemo- or radiotherapy. Measure of caspase-3 activity in 9L (A) and C6 (C) cells. Measure of PS externalisation by AnnexinV–FITC binding assay on 9L (B) and C6 cells (D). Total percentages of 9L (E) and C6 (F) binucleated cells with MN. Significantly different from the control: *P<0.05, **P<0.01.

Mentions: We have investigated and compared in vitro apoptosis induction and MN formation in p53 mutated 9L or p53 wild-type C6 cells treated either by irradiation or by chemotherapeutic agents (chosen according to their opposite efficacies on tumour cell viability, data not shown). Apoptosis was quantified by relevant tests addressing different stages of the process: measure of caspase-3 activity (Figure 1A and CFigure 1


Micronuclei to detect in vivo chemotherapy damage in a p53 mutated solid tumour.

Driessens G, Harsan L, Robaye B, Waroquier D, Browaeys P, Giannakopoulos X, Velu T, Bruyns C - Br. J. Cancer (2003)

In vitro induction of apoptosis or MN formation after chemo- or radiotherapy. Measure of caspase-3 activity in 9L (A) and C6 (C) cells. Measure of PS externalisation by AnnexinV–FITC binding assay on 9L (B) and C6 cells (D). Total percentages of 9L (E) and C6 (F) binucleated cells with MN. Significantly different from the control: *P<0.05, **P<0.01.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376913&req=5

fig1: In vitro induction of apoptosis or MN formation after chemo- or radiotherapy. Measure of caspase-3 activity in 9L (A) and C6 (C) cells. Measure of PS externalisation by AnnexinV–FITC binding assay on 9L (B) and C6 cells (D). Total percentages of 9L (E) and C6 (F) binucleated cells with MN. Significantly different from the control: *P<0.05, **P<0.01.
Mentions: We have investigated and compared in vitro apoptosis induction and MN formation in p53 mutated 9L or p53 wild-type C6 cells treated either by irradiation or by chemotherapeutic agents (chosen according to their opposite efficacies on tumour cell viability, data not shown). Apoptosis was quantified by relevant tests addressing different stages of the process: measure of caspase-3 activity (Figure 1A and CFigure 1

Bottom Line: Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity.In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status.In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.

View Article: PubMed Central - PubMed

Affiliation: Interdisciplinary Research Institute (IRIBHM), Faculty of Medicine, Université Libre de Bruxelles, 1070 Brussels, Belgium.

ABSTRACT
Apoptosis induction and micronuclei formation were compared following cytotoxic treatments in two rat glioma differing in p53 integrity. In vitro, micronuclei emergence but not apoptosis was linked to the p53 mutated status. In vivo, micronuclei assays were more sensitive to evaluate DNA damage induced by chemotherapy in a p53-mutated solid tumour.

Show MeSH
Related in: MedlinePlus