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Irradiation differentially affects substratum-dependent survival, adhesion, and invasion of glioblastoma cell lines.

Cordes N, Hansmeier B, Beinke C, Meineke V, van Beuningen D - Br. J. Cancer (2003)

Bottom Line: In particular, the aggressiveness of glioblastoma multiforme has been associated with tumour cell invasion into adjacent normal brain tissue.Major findings of this study include a significantly increased survival of irradiated A-172 but not of irradiated U-138, LN-229, and LN-18 cells on FN or Matrigel compared to cells irradiated on polystyrene or BSA.This integrin induction caused improved cell adhesion to FN or Matrigel.

View Article: PubMed Central - PubMed

Affiliation: Institute of Radiobiology, German Armed Forces, Neuherbergstrasse 11, 80937 Munich, Germany. nils_cordes@web.de

ABSTRACT
Effects of ionising radiation on extracellular matrix (ECM)-modulated cell survival and on adhesion and invasion are not well understood. In particular, the aggressiveness of glioblastoma multiforme has been associated with tumour cell invasion into adjacent normal brain tissue. To examine these effects in more depth, four human glioblastoma cell lines (A-172, U-138, LN-229 and LN-18) were irradiated on fibronectin (FN), Matrigel, BSA or polystyrene. Major findings of this study include a significantly increased survival of irradiated A-172 but not of irradiated U-138, LN-229, and LN-18 cells on FN or Matrigel compared to cells irradiated on polystyrene or BSA. Irradiation induced a dose-dependent increase in functional beta 1- and beta 3-integrins in all four glioma cell lines. This integrin induction caused improved cell adhesion to FN or Matrigel. In contrast to U-138, LN-229 and LN-18 cells, irradiation strongly impaired A-172 cell invasion. Invasion of all cell lines was inhibited by anti-integrin antibodies, the disintegrin echistatin and the MMP-2/-9 inhibitor III. Additionally, beta 1- and beta 3-integrins modulated basal and radiation-altered gelatinolytic activity of MMP-2. Tested glioblastoma cell lines showed a differential cellular susceptibility to FN or Matrigel which affected the cellular radiosensitivity. Three out of four glioma cell lines demonstrated a combination of a substratum-independent survival after irradiation and an invasive potential which was not affected by irradiation. beta 1- and beta 3-integrins were identified to play a substantial, regulatory role in survival, adhesion, invasion and MMP-2 activity. Detailed insights into radioresistance and invasion processes might offer new therapeutic strategies to enhance cell killing of lethal high-grade astrocytoma.

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Double immunofluorescence staining of β1-integrin plus MMP-2 in A-172 and U-138 cells. β1-integrin and MMP-2 were visualised by confocal scanning microscopy after fixing and staining of cells with the appropriate antibodies. Arrows indicate β1-integrin plus MMP-2 colocalisation within focal adhesion sites in the cell membrane. Bar, 10 μm.
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fig6: Double immunofluorescence staining of β1-integrin plus MMP-2 in A-172 and U-138 cells. β1-integrin and MMP-2 were visualised by confocal scanning microscopy after fixing and staining of cells with the appropriate antibodies. Arrows indicate β1-integrin plus MMP-2 colocalisation within focal adhesion sites in the cell membrane. Bar, 10 μm.

Mentions: The fluorescence signals of β1-integrins in combination with MMP-2 colocalised at these specific membrane regions termed focal adhesions and in the cytoplasm (Figure 6Figure 6


Irradiation differentially affects substratum-dependent survival, adhesion, and invasion of glioblastoma cell lines.

Cordes N, Hansmeier B, Beinke C, Meineke V, van Beuningen D - Br. J. Cancer (2003)

Double immunofluorescence staining of β1-integrin plus MMP-2 in A-172 and U-138 cells. β1-integrin and MMP-2 were visualised by confocal scanning microscopy after fixing and staining of cells with the appropriate antibodies. Arrows indicate β1-integrin plus MMP-2 colocalisation within focal adhesion sites in the cell membrane. Bar, 10 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376852&req=5

fig6: Double immunofluorescence staining of β1-integrin plus MMP-2 in A-172 and U-138 cells. β1-integrin and MMP-2 were visualised by confocal scanning microscopy after fixing and staining of cells with the appropriate antibodies. Arrows indicate β1-integrin plus MMP-2 colocalisation within focal adhesion sites in the cell membrane. Bar, 10 μm.
Mentions: The fluorescence signals of β1-integrins in combination with MMP-2 colocalised at these specific membrane regions termed focal adhesions and in the cytoplasm (Figure 6Figure 6

Bottom Line: In particular, the aggressiveness of glioblastoma multiforme has been associated with tumour cell invasion into adjacent normal brain tissue.Major findings of this study include a significantly increased survival of irradiated A-172 but not of irradiated U-138, LN-229, and LN-18 cells on FN or Matrigel compared to cells irradiated on polystyrene or BSA.This integrin induction caused improved cell adhesion to FN or Matrigel.

View Article: PubMed Central - PubMed

Affiliation: Institute of Radiobiology, German Armed Forces, Neuherbergstrasse 11, 80937 Munich, Germany. nils_cordes@web.de

ABSTRACT
Effects of ionising radiation on extracellular matrix (ECM)-modulated cell survival and on adhesion and invasion are not well understood. In particular, the aggressiveness of glioblastoma multiforme has been associated with tumour cell invasion into adjacent normal brain tissue. To examine these effects in more depth, four human glioblastoma cell lines (A-172, U-138, LN-229 and LN-18) were irradiated on fibronectin (FN), Matrigel, BSA or polystyrene. Major findings of this study include a significantly increased survival of irradiated A-172 but not of irradiated U-138, LN-229, and LN-18 cells on FN or Matrigel compared to cells irradiated on polystyrene or BSA. Irradiation induced a dose-dependent increase in functional beta 1- and beta 3-integrins in all four glioma cell lines. This integrin induction caused improved cell adhesion to FN or Matrigel. In contrast to U-138, LN-229 and LN-18 cells, irradiation strongly impaired A-172 cell invasion. Invasion of all cell lines was inhibited by anti-integrin antibodies, the disintegrin echistatin and the MMP-2/-9 inhibitor III. Additionally, beta 1- and beta 3-integrins modulated basal and radiation-altered gelatinolytic activity of MMP-2. Tested glioblastoma cell lines showed a differential cellular susceptibility to FN or Matrigel which affected the cellular radiosensitivity. Three out of four glioma cell lines demonstrated a combination of a substratum-independent survival after irradiation and an invasive potential which was not affected by irradiation. beta 1- and beta 3-integrins were identified to play a substantial, regulatory role in survival, adhesion, invasion and MMP-2 activity. Detailed insights into radioresistance and invasion processes might offer new therapeutic strategies to enhance cell killing of lethal high-grade astrocytoma.

Show MeSH
Related in: MedlinePlus