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Tyrosine-kinase expression profiles in human gastric cancer cell lines and their modulations with retinoic acids.

Kao HW, Chen HC, Wu CW, Lin WC - Br. J. Cancer (2003)

Bottom Line: Many protein tyrosine kinases are key regulators involved in cellular growth, differentiation, development, apoptosis and signal transduction pathways.Expression of tyrosine kinases in retionic acid-treated cancer cells was investigated by reverse trancriptase-polymerase chain reaction (RT-PCR) and a novel restriction analysis of gene expression (RAGE) display technique.In cells treated with 9-cis-retinoic acid or all-trans-retinoic acid, we found that two PTKs (Eph and Hek5) appeared to be upregulated.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.

ABSTRACT
Many protein tyrosine kinases are key regulators involved in cellular growth, differentiation, development, apoptosis and signal transduction pathways. Obtaining a comprehensive tyrosine-kinase expression profile in tumour cells is essential to learning more about their oncogenic potentials and responses to various chemotherapeutic reagents - such as retinoic acid, which has been shown to suppress the growth of gastric cancer cells and modulate gene expression. Expression of tyrosine kinases in retionic acid-treated cancer cells was investigated by reverse trancriptase-polymerase chain reaction (RT-PCR) and a novel restriction analysis of gene expression (RAGE) display technique. We first established comprehensive tyrosine-kinase profiles in different human gastric cancer cell lines. In cells treated with 9-cis-retinoic acid or all-trans-retinoic acid, we found that two PTKs (Eph and Hek5) appeared to be upregulated. In the present study, we demonstrate an efficient and simple RAGE approach for examining tyrosine kinases' expression in tumour cells and their alterations following drug treatments.

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Related in: MedlinePlus

Immunoblot showing the expression of yes tyrosine kinase in human gastric cancer cell lines. An equal amount of cell lysate from human gastric cancer cell lines was resolved by SDS–PAGE. After electrophoresis, proteins were transferred to a PVDF nylon membrane and then probed with anti-yes antibody. The anti-β-actin antibody was used a control.
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fig4: Immunoblot showing the expression of yes tyrosine kinase in human gastric cancer cell lines. An equal amount of cell lysate from human gastric cancer cell lines was resolved by SDS–PAGE. After electrophoresis, proteins were transferred to a PVDF nylon membrane and then probed with anti-yes antibody. The anti-β-actin antibody was used a control.

Mentions: RAGE profile and RT–PCR pattern of yes tyrosine kinase in human gastric cancer cell lines. Upper panel: RAGE expression profile of the yes kinase gene. Lower panel: yes kinase expression detected by RT–PCR method with specific primers.


Tyrosine-kinase expression profiles in human gastric cancer cell lines and their modulations with retinoic acids.

Kao HW, Chen HC, Wu CW, Lin WC - Br. J. Cancer (2003)

Immunoblot showing the expression of yes tyrosine kinase in human gastric cancer cell lines. An equal amount of cell lysate from human gastric cancer cell lines was resolved by SDS–PAGE. After electrophoresis, proteins were transferred to a PVDF nylon membrane and then probed with anti-yes antibody. The anti-β-actin antibody was used a control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376380&req=5

fig4: Immunoblot showing the expression of yes tyrosine kinase in human gastric cancer cell lines. An equal amount of cell lysate from human gastric cancer cell lines was resolved by SDS–PAGE. After electrophoresis, proteins were transferred to a PVDF nylon membrane and then probed with anti-yes antibody. The anti-β-actin antibody was used a control.
Mentions: RAGE profile and RT–PCR pattern of yes tyrosine kinase in human gastric cancer cell lines. Upper panel: RAGE expression profile of the yes kinase gene. Lower panel: yes kinase expression detected by RT–PCR method with specific primers.

Bottom Line: Many protein tyrosine kinases are key regulators involved in cellular growth, differentiation, development, apoptosis and signal transduction pathways.Expression of tyrosine kinases in retionic acid-treated cancer cells was investigated by reverse trancriptase-polymerase chain reaction (RT-PCR) and a novel restriction analysis of gene expression (RAGE) display technique.In cells treated with 9-cis-retinoic acid or all-trans-retinoic acid, we found that two PTKs (Eph and Hek5) appeared to be upregulated.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, ROC.

ABSTRACT
Many protein tyrosine kinases are key regulators involved in cellular growth, differentiation, development, apoptosis and signal transduction pathways. Obtaining a comprehensive tyrosine-kinase expression profile in tumour cells is essential to learning more about their oncogenic potentials and responses to various chemotherapeutic reagents - such as retinoic acid, which has been shown to suppress the growth of gastric cancer cells and modulate gene expression. Expression of tyrosine kinases in retionic acid-treated cancer cells was investigated by reverse trancriptase-polymerase chain reaction (RT-PCR) and a novel restriction analysis of gene expression (RAGE) display technique. We first established comprehensive tyrosine-kinase profiles in different human gastric cancer cell lines. In cells treated with 9-cis-retinoic acid or all-trans-retinoic acid, we found that two PTKs (Eph and Hek5) appeared to be upregulated. In the present study, we demonstrate an efficient and simple RAGE approach for examining tyrosine kinases' expression in tumour cells and their alterations following drug treatments.

Show MeSH
Related in: MedlinePlus