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Early detection of tumour immune-rejection using magnetic resonance imaging.

Hu DE, Beauregard DA, Bearchell MC, Thomsen LL, Brindle KM - Br. J. Cancer (2003)

Bottom Line: Dynamic contrast agent-enhanced magnetic resonance imaging measurements of the perfusion of an immunogenic murine tumour showed that immune rejection was preceded by an increase in the apparent vascular volume of the tumour.This increase in vascularity, which has been observed previously in other tumours undergoing immune rejection, was confirmed by histological analysis of tumour sections obtained postmortem.Magnetic resonance imaging measurements similar to this could be used in the clinic to monitor the early responses of tumours to immunotherapy, before there is any change in tumour growth rate or volume.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Cambridge, UK.

ABSTRACT
Dynamic contrast agent-enhanced magnetic resonance imaging measurements of the perfusion of an immunogenic murine tumour showed that immune rejection was preceded by an increase in the apparent vascular volume of the tumour. This increase in vascularity, which has been observed previously in other tumours undergoing immune rejection, was confirmed by histological analysis of tumour sections obtained postmortem. Magnetic resonance imaging measurements similar to this could be used in the clinic to monitor the early responses of tumours to immunotherapy, before there is any change in tumour growth rate or volume.

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Histological features of spontaneous regression in E.G7-OVA tumours. Tumour sections (5 μm thick) were stained with Masson's trichrome (A) or carmine red dye counterstained with light green (B). In A (ii and iii) the tumour cells can be seen to be surrounded by fibroblasts and capillaries filled with red blood cells (arrowed). There is also evidence of collagen deposition, which stains green with this dye (arrowed in A iii). A(i) is a representative section from an EL-4 tumour. The tumour sections shown in B (ii and iii) were obtained following injection of the animal with carmine dye. Functional vessels are stained red in these sections (arrowed). B(i) is a representative section from an EL-4 tumour. The bars are 400 μm long.
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fig2: Histological features of spontaneous regression in E.G7-OVA tumours. Tumour sections (5 μm thick) were stained with Masson's trichrome (A) or carmine red dye counterstained with light green (B). In A (ii and iii) the tumour cells can be seen to be surrounded by fibroblasts and capillaries filled with red blood cells (arrowed). There is also evidence of collagen deposition, which stains green with this dye (arrowed in A iii). A(i) is a representative section from an EL-4 tumour. The tumour sections shown in B (ii and iii) were obtained following injection of the animal with carmine dye. Functional vessels are stained red in these sections (arrowed). B(i) is a representative section from an EL-4 tumour. The bars are 400 μm long.

Mentions: In addition to the lymphocytic infiltrate observed in regressing E.G7-OVA tumours, there was also clear evidence of an angiofibroblastic response (Bodurtha et al, 1976; Lynch et al, 1978; Martin et al, 1987; Shaw et al, 1989; Vaage, 1992), with proliferation of fibroblasts and capillary vessels and encapsulation of the tumour in a fibrous, collagen containing, capsule (see Figure 2Figure 2


Early detection of tumour immune-rejection using magnetic resonance imaging.

Hu DE, Beauregard DA, Bearchell MC, Thomsen LL, Brindle KM - Br. J. Cancer (2003)

Histological features of spontaneous regression in E.G7-OVA tumours. Tumour sections (5 μm thick) were stained with Masson's trichrome (A) or carmine red dye counterstained with light green (B). In A (ii and iii) the tumour cells can be seen to be surrounded by fibroblasts and capillaries filled with red blood cells (arrowed). There is also evidence of collagen deposition, which stains green with this dye (arrowed in A iii). A(i) is a representative section from an EL-4 tumour. The tumour sections shown in B (ii and iii) were obtained following injection of the animal with carmine dye. Functional vessels are stained red in these sections (arrowed). B(i) is a representative section from an EL-4 tumour. The bars are 400 μm long.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC2376373&req=5

fig2: Histological features of spontaneous regression in E.G7-OVA tumours. Tumour sections (5 μm thick) were stained with Masson's trichrome (A) or carmine red dye counterstained with light green (B). In A (ii and iii) the tumour cells can be seen to be surrounded by fibroblasts and capillaries filled with red blood cells (arrowed). There is also evidence of collagen deposition, which stains green with this dye (arrowed in A iii). A(i) is a representative section from an EL-4 tumour. The tumour sections shown in B (ii and iii) were obtained following injection of the animal with carmine dye. Functional vessels are stained red in these sections (arrowed). B(i) is a representative section from an EL-4 tumour. The bars are 400 μm long.
Mentions: In addition to the lymphocytic infiltrate observed in regressing E.G7-OVA tumours, there was also clear evidence of an angiofibroblastic response (Bodurtha et al, 1976; Lynch et al, 1978; Martin et al, 1987; Shaw et al, 1989; Vaage, 1992), with proliferation of fibroblasts and capillary vessels and encapsulation of the tumour in a fibrous, collagen containing, capsule (see Figure 2Figure 2

Bottom Line: Dynamic contrast agent-enhanced magnetic resonance imaging measurements of the perfusion of an immunogenic murine tumour showed that immune rejection was preceded by an increase in the apparent vascular volume of the tumour.This increase in vascularity, which has been observed previously in other tumours undergoing immune rejection, was confirmed by histological analysis of tumour sections obtained postmortem.Magnetic resonance imaging measurements similar to this could be used in the clinic to monitor the early responses of tumours to immunotherapy, before there is any change in tumour growth rate or volume.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Cambridge, UK.

ABSTRACT
Dynamic contrast agent-enhanced magnetic resonance imaging measurements of the perfusion of an immunogenic murine tumour showed that immune rejection was preceded by an increase in the apparent vascular volume of the tumour. This increase in vascularity, which has been observed previously in other tumours undergoing immune rejection, was confirmed by histological analysis of tumour sections obtained postmortem. Magnetic resonance imaging measurements similar to this could be used in the clinic to monitor the early responses of tumours to immunotherapy, before there is any change in tumour growth rate or volume.

Show MeSH
Related in: MedlinePlus