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Collagen crosslink excretion and staging of oral cancer.

Springer IN, Terheyden H, Dunsche A, Czech N, Suhr MA, Tiemann M, Hedderich J, Açil Y - Br. J. Cancer (2003)

Bottom Line: There was a significant difference in the average urinary levels of LP and HP between groups 1-4 (P<0.001).Presence of tumour tissue could be detected with a sensitivity of 90%.In conclusion, a normal LP concentration in patients with an OSCC strongly suggests that bone invasion by the disease has not taken place.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, University of Kiel, Germany. springer@mkg.uni-kiel.de

ABSTRACT
Lysylpyridinoline (LP) and hydroxylysylpyridinoline (HP) are collagen crosslink residues of which the urinary concentration reflects the level of connective-tissue turnover. HP is ubiquitous in tissue, whereas LP is specific for bone. The purpose of this investigation was to assess the sensitivity and specificity of an increased urinary concentration of both HP and LP in indicating infiltration of mandibular bone by an oral squamous cell carcinoma (OSCC) or recurrence of the disease after successful therapy. We investigated the history and urine levels in 116 adult patients, who were divided into the following groups. Group 1: patients with OSCC with bone infiltration (n=17); group 2: patients with confirmed OSCC (n=12) without evidence of bone infiltration; group 3: patients with recurrence of an OSCC (n=13); group 4: patients without clinical evidence of disease (n=74). The range and upper limit of normal values (HP(max) and LP(max)) were measured from the normal controls in group 4. Levels of LP and HP were measured by HPLC and fluorescence detection. There was a significant difference in the average urinary levels of LP and HP between groups 1-4 (P<0.001). The presence of mandibular bone infiltration could be detected with a sensitivity and specificity of 100% when comparing groups 1 and 2. Presence of tumour tissue could be detected with a sensitivity of 90%. In conclusion, a normal LP concentration in patients with an OSCC strongly suggests that bone invasion by the disease has not taken place. If both urinary HP and LP are elevated, disease recurrence is highly likely.

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(A,B) Scintigraphy of a patient with an OSCC with (A) and without (B) infiltration of the mandibular bone. Technetium 99m MDP bone scans with planar imaging and SPECT were performed in the course of the staging. No increased activity is visible in (A), but a lesion of the right mandible is seen in (B) (arrow). For the chromatograms of these patients see Figure 3A and B.
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fig2: (A,B) Scintigraphy of a patient with an OSCC with (A) and without (B) infiltration of the mandibular bone. Technetium 99m MDP bone scans with planar imaging and SPECT were performed in the course of the staging. No increased activity is visible in (A), but a lesion of the right mandible is seen in (B) (arrow). For the chromatograms of these patients see Figure 3A and B.

Mentions: HPmax was 95 nmol mmol−1 creatinine and LPmax 20 nmol mmol−1 creatinine. Differences between the average concentrations of HP and LP of groups 1–4 were statistically significant (ANOVA: P<0.001, Kruskal–Wallis: P<0.001). The average urinary HP and LP concentrations were significantly elevated in group 1 compared to group 2 (P<0.001 for HP and LP), in group 1 compared to group 4 (P<0.001 for HP and LP) and in group 3 compared to group 4 (P=0.001 for HP and P<0.01 for LP) (Figures 1, 2Figure 2


Collagen crosslink excretion and staging of oral cancer.

Springer IN, Terheyden H, Dunsche A, Czech N, Suhr MA, Tiemann M, Hedderich J, Açil Y - Br. J. Cancer (2003)

(A,B) Scintigraphy of a patient with an OSCC with (A) and without (B) infiltration of the mandibular bone. Technetium 99m MDP bone scans with planar imaging and SPECT were performed in the course of the staging. No increased activity is visible in (A), but a lesion of the right mandible is seen in (B) (arrow). For the chromatograms of these patients see Figure 3A and B.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376365&req=5

fig2: (A,B) Scintigraphy of a patient with an OSCC with (A) and without (B) infiltration of the mandibular bone. Technetium 99m MDP bone scans with planar imaging and SPECT were performed in the course of the staging. No increased activity is visible in (A), but a lesion of the right mandible is seen in (B) (arrow). For the chromatograms of these patients see Figure 3A and B.
Mentions: HPmax was 95 nmol mmol−1 creatinine and LPmax 20 nmol mmol−1 creatinine. Differences between the average concentrations of HP and LP of groups 1–4 were statistically significant (ANOVA: P<0.001, Kruskal–Wallis: P<0.001). The average urinary HP and LP concentrations were significantly elevated in group 1 compared to group 2 (P<0.001 for HP and LP), in group 1 compared to group 4 (P<0.001 for HP and LP) and in group 3 compared to group 4 (P=0.001 for HP and P<0.01 for LP) (Figures 1, 2Figure 2

Bottom Line: There was a significant difference in the average urinary levels of LP and HP between groups 1-4 (P<0.001).Presence of tumour tissue could be detected with a sensitivity of 90%.In conclusion, a normal LP concentration in patients with an OSCC strongly suggests that bone invasion by the disease has not taken place.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, University of Kiel, Germany. springer@mkg.uni-kiel.de

ABSTRACT
Lysylpyridinoline (LP) and hydroxylysylpyridinoline (HP) are collagen crosslink residues of which the urinary concentration reflects the level of connective-tissue turnover. HP is ubiquitous in tissue, whereas LP is specific for bone. The purpose of this investigation was to assess the sensitivity and specificity of an increased urinary concentration of both HP and LP in indicating infiltration of mandibular bone by an oral squamous cell carcinoma (OSCC) or recurrence of the disease after successful therapy. We investigated the history and urine levels in 116 adult patients, who were divided into the following groups. Group 1: patients with OSCC with bone infiltration (n=17); group 2: patients with confirmed OSCC (n=12) without evidence of bone infiltration; group 3: patients with recurrence of an OSCC (n=13); group 4: patients without clinical evidence of disease (n=74). The range and upper limit of normal values (HP(max) and LP(max)) were measured from the normal controls in group 4. Levels of LP and HP were measured by HPLC and fluorescence detection. There was a significant difference in the average urinary levels of LP and HP between groups 1-4 (P<0.001). The presence of mandibular bone infiltration could be detected with a sensitivity and specificity of 100% when comparing groups 1 and 2. Presence of tumour tissue could be detected with a sensitivity of 90%. In conclusion, a normal LP concentration in patients with an OSCC strongly suggests that bone invasion by the disease has not taken place. If both urinary HP and LP are elevated, disease recurrence is highly likely.

Show MeSH
Related in: MedlinePlus