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Induction of vasculogenesis in breast cancer models.

Shirakawa K, Furuhata S, Watanabe I, Hayase H, Shimizu A, Ikarashi Y, Yoshida T, Terada M, Hashimoto D, Wakasugi H - Br. J. Cancer (2002)

Bottom Line: The endothelial precursor cell population was 14% or greater in the gated lymphocyte-size fraction of the inflammatory breast cancer xenograft named WIBC-9, which exhibits a hypervascular structure and de novo formation of vascular channels, namely vasculogenic mimicry (Shirakawa et al, 2001).Furthermore, they demonstrated significantly higher levels of murine vascular endothelial growth factor and interleukin-8 in culture supernatant on time course by enzyme-linked immunosorbent assay.These findings constitute direct evidence that breast cancer induces postnatal vasculogenesis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-Ku, Tokyo 104-0045, Japan. hwakasug@gan2.ncc.go.jp

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Related in: MedlinePlus

BM derived CD34++Flk-1+ MNC subset and CD34++Flk-1− MNC subset (1×105 cells well−1) were sorted by using FACS (n=5) and incubated for 14 days at 37°C, and the supernatants were collected every 12 h and stored at −80°C until used. Concentrations of mVEGF and mIL-8 were measured by ELISA in 100 μl samples of supernatant, using Immunoassay Kits (Immuno-Biological Laboratories Co., Ltd., Fujioka, Japan). Each assay was performed in triplicate.
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fig5: BM derived CD34++Flk-1+ MNC subset and CD34++Flk-1− MNC subset (1×105 cells well−1) were sorted by using FACS (n=5) and incubated for 14 days at 37°C, and the supernatants were collected every 12 h and stored at −80°C until used. Concentrations of mVEGF and mIL-8 were measured by ELISA in 100 μl samples of supernatant, using Immunoassay Kits (Immuno-Biological Laboratories Co., Ltd., Fujioka, Japan). Each assay was performed in triplicate.

Mentions: (A) HUVECs and BM-derived EPCs (mCD34+ and mFlt-1+) formed capillary networks. (B) BM-derived EPCs, which incorporated DiI-labelled acLDL, were integrated with HUVECs. Phase contrast photomicrographs showed capillary networks. Each scale bar is 10 μm.


Induction of vasculogenesis in breast cancer models.

Shirakawa K, Furuhata S, Watanabe I, Hayase H, Shimizu A, Ikarashi Y, Yoshida T, Terada M, Hashimoto D, Wakasugi H - Br. J. Cancer (2002)

BM derived CD34++Flk-1+ MNC subset and CD34++Flk-1− MNC subset (1×105 cells well−1) were sorted by using FACS (n=5) and incubated for 14 days at 37°C, and the supernatants were collected every 12 h and stored at −80°C until used. Concentrations of mVEGF and mIL-8 were measured by ELISA in 100 μl samples of supernatant, using Immunoassay Kits (Immuno-Biological Laboratories Co., Ltd., Fujioka, Japan). Each assay was performed in triplicate.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376301&req=5

fig5: BM derived CD34++Flk-1+ MNC subset and CD34++Flk-1− MNC subset (1×105 cells well−1) were sorted by using FACS (n=5) and incubated for 14 days at 37°C, and the supernatants were collected every 12 h and stored at −80°C until used. Concentrations of mVEGF and mIL-8 were measured by ELISA in 100 μl samples of supernatant, using Immunoassay Kits (Immuno-Biological Laboratories Co., Ltd., Fujioka, Japan). Each assay was performed in triplicate.
Mentions: (A) HUVECs and BM-derived EPCs (mCD34+ and mFlt-1+) formed capillary networks. (B) BM-derived EPCs, which incorporated DiI-labelled acLDL, were integrated with HUVECs. Phase contrast photomicrographs showed capillary networks. Each scale bar is 10 μm.

Bottom Line: The endothelial precursor cell population was 14% or greater in the gated lymphocyte-size fraction of the inflammatory breast cancer xenograft named WIBC-9, which exhibits a hypervascular structure and de novo formation of vascular channels, namely vasculogenic mimicry (Shirakawa et al, 2001).Furthermore, they demonstrated significantly higher levels of murine vascular endothelial growth factor and interleukin-8 in culture supernatant on time course by enzyme-linked immunosorbent assay.These findings constitute direct evidence that breast cancer induces postnatal vasculogenesis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-Ku, Tokyo 104-0045, Japan. hwakasug@gan2.ncc.go.jp

Show MeSH
Related in: MedlinePlus