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Induction of vasculogenesis in breast cancer models.

Shirakawa K, Furuhata S, Watanabe I, Hayase H, Shimizu A, Ikarashi Y, Yoshida T, Terada M, Hashimoto D, Wakasugi H - Br. J. Cancer (2002)

Bottom Line: The endothelial precursor cell population was 14% or greater in the gated lymphocyte-size fraction of the inflammatory breast cancer xenograft named WIBC-9, which exhibits a hypervascular structure and de novo formation of vascular channels, namely vasculogenic mimicry (Shirakawa et al, 2001).Furthermore, they demonstrated significantly higher levels of murine vascular endothelial growth factor and interleukin-8 in culture supernatant on time course by enzyme-linked immunosorbent assay.These findings constitute direct evidence that breast cancer induces postnatal vasculogenesis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-Ku, Tokyo 104-0045, Japan. hwakasug@gan2.ncc.go.jp

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(A) HUVECs and BM-derived EPCs (mCD34+ and mFlt-1+) formed capillary networks. (B) BM-derived EPCs, which incorporated DiI-labelled acLDL, were integrated with HUVECs. Phase contrast photomicrographs showed capillary networks. Each scale bar is 10 μm.
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fig4: (A) HUVECs and BM-derived EPCs (mCD34+ and mFlt-1+) formed capillary networks. (B) BM-derived EPCs, which incorporated DiI-labelled acLDL, were integrated with HUVECs. Phase contrast photomicrographs showed capillary networks. Each scale bar is 10 μm.

Mentions: On day 4 after plating the CD34+ floating MNCs, spindle-shaped AT cells and cluster formation were clearly visible in WIBC-9 samples. On day 13, WIBC-9 AT cells were found to uptake DiI-labelled acLDL. Immunocytochemistry revealed that these cells were negative for Mac-1, indicating that they were not of a monocyte lineage.


Induction of vasculogenesis in breast cancer models.

Shirakawa K, Furuhata S, Watanabe I, Hayase H, Shimizu A, Ikarashi Y, Yoshida T, Terada M, Hashimoto D, Wakasugi H - Br. J. Cancer (2002)

(A) HUVECs and BM-derived EPCs (mCD34+ and mFlt-1+) formed capillary networks. (B) BM-derived EPCs, which incorporated DiI-labelled acLDL, were integrated with HUVECs. Phase contrast photomicrographs showed capillary networks. Each scale bar is 10 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376301&req=5

fig4: (A) HUVECs and BM-derived EPCs (mCD34+ and mFlt-1+) formed capillary networks. (B) BM-derived EPCs, which incorporated DiI-labelled acLDL, were integrated with HUVECs. Phase contrast photomicrographs showed capillary networks. Each scale bar is 10 μm.
Mentions: On day 4 after plating the CD34+ floating MNCs, spindle-shaped AT cells and cluster formation were clearly visible in WIBC-9 samples. On day 13, WIBC-9 AT cells were found to uptake DiI-labelled acLDL. Immunocytochemistry revealed that these cells were negative for Mac-1, indicating that they were not of a monocyte lineage.

Bottom Line: The endothelial precursor cell population was 14% or greater in the gated lymphocyte-size fraction of the inflammatory breast cancer xenograft named WIBC-9, which exhibits a hypervascular structure and de novo formation of vascular channels, namely vasculogenic mimicry (Shirakawa et al, 2001).Furthermore, they demonstrated significantly higher levels of murine vascular endothelial growth factor and interleukin-8 in culture supernatant on time course by enzyme-linked immunosorbent assay.These findings constitute direct evidence that breast cancer induces postnatal vasculogenesis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-Ku, Tokyo 104-0045, Japan. hwakasug@gan2.ncc.go.jp

Show MeSH
Related in: MedlinePlus