Induction of vasculogenesis in breast cancer models.
Bottom Line: The endothelial precursor cell population was 14% or greater in the gated lymphocyte-size fraction of the inflammatory breast cancer xenograft named WIBC-9, which exhibits a hypervascular structure and de novo formation of vascular channels, namely vasculogenic mimicry (Shirakawa et al, 2001).Furthermore, they demonstrated significantly higher levels of murine vascular endothelial growth factor and interleukin-8 in culture supernatant on time course by enzyme-linked immunosorbent assay.These findings constitute direct evidence that breast cancer induces postnatal vasculogenesis in vivo.
Affiliation: Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-Ku, Tokyo 104-0045, Japan. email@example.comShow MeSH
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Mentions: On day 4 after plating the CD34+ floating MNCs, spindle-shaped AT cells and cluster formation were clearly visible in WIBC-9 samples. On day 13, WIBC-9 AT cells were found to uptake DiI-labelled acLDL. Immunocytochemistry revealed that these cells were negative for Mac-1, indicating that they were not of a monocyte lineage.
Affiliation: Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-Ku, Tokyo 104-0045, Japan. firstname.lastname@example.org