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Induction of vasculogenesis in breast cancer models.

Shirakawa K, Furuhata S, Watanabe I, Hayase H, Shimizu A, Ikarashi Y, Yoshida T, Terada M, Hashimoto D, Wakasugi H - Br. J. Cancer (2002)

Bottom Line: The endothelial precursor cell population was 14% or greater in the gated lymphocyte-size fraction of the inflammatory breast cancer xenograft named WIBC-9, which exhibits a hypervascular structure and de novo formation of vascular channels, namely vasculogenic mimicry (Shirakawa et al, 2001).Furthermore, they demonstrated significantly higher levels of murine vascular endothelial growth factor and interleukin-8 in culture supernatant on time course by enzyme-linked immunosorbent assay.These findings constitute direct evidence that breast cancer induces postnatal vasculogenesis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-Ku, Tokyo 104-0045, Japan. hwakasug@gan2.ncc.go.jp

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Related in: MedlinePlus

(A) AT cell population in the xenografts. AT cells, including ECs were counted. In WIBC-9 samples, the AT cell population was high. AT populations in other cancer lines were higher than in the control, a finding consistent with the size of the CD34+/Flk-1+ MNC population. (B) BM-derived EPC morphogenesis on murine fibronectin. On day 4 after plating CD34+ floating MNCs, spindle-shaped AT cells and cluster formation were evident in WIBC-9 samples. On day 13, all AT cells were found to uptake DiI-labelled acLDL. Immunocytochemistry revealed that all cells were Mac-1–negative. Each scale bar is 50 μm.
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fig3: (A) AT cell population in the xenografts. AT cells, including ECs were counted. In WIBC-9 samples, the AT cell population was high. AT populations in other cancer lines were higher than in the control, a finding consistent with the size of the CD34+/Flk-1+ MNC population. (B) BM-derived EPC morphogenesis on murine fibronectin. On day 4 after plating CD34+ floating MNCs, spindle-shaped AT cells and cluster formation were evident in WIBC-9 samples. On day 13, all AT cells were found to uptake DiI-labelled acLDL. Immunocytochemistry revealed that all cells were Mac-1–negative. Each scale bar is 50 μm.

Mentions: EPCs(mCD34+, mFlk-1+) in the bone marrow and peripheral blood in WIBC-9, included a large number of FLT1+(98.9%/97.5%), and TIE2+(71.8%/64.2%) but include small number of VE-cadherin (23.2%/30.6%) and CD31+(20.9%/20.9%).


Induction of vasculogenesis in breast cancer models.

Shirakawa K, Furuhata S, Watanabe I, Hayase H, Shimizu A, Ikarashi Y, Yoshida T, Terada M, Hashimoto D, Wakasugi H - Br. J. Cancer (2002)

(A) AT cell population in the xenografts. AT cells, including ECs were counted. In WIBC-9 samples, the AT cell population was high. AT populations in other cancer lines were higher than in the control, a finding consistent with the size of the CD34+/Flk-1+ MNC population. (B) BM-derived EPC morphogenesis on murine fibronectin. On day 4 after plating CD34+ floating MNCs, spindle-shaped AT cells and cluster formation were evident in WIBC-9 samples. On day 13, all AT cells were found to uptake DiI-labelled acLDL. Immunocytochemistry revealed that all cells were Mac-1–negative. Each scale bar is 50 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC2376301&req=5

fig3: (A) AT cell population in the xenografts. AT cells, including ECs were counted. In WIBC-9 samples, the AT cell population was high. AT populations in other cancer lines were higher than in the control, a finding consistent with the size of the CD34+/Flk-1+ MNC population. (B) BM-derived EPC morphogenesis on murine fibronectin. On day 4 after plating CD34+ floating MNCs, spindle-shaped AT cells and cluster formation were evident in WIBC-9 samples. On day 13, all AT cells were found to uptake DiI-labelled acLDL. Immunocytochemistry revealed that all cells were Mac-1–negative. Each scale bar is 50 μm.
Mentions: EPCs(mCD34+, mFlk-1+) in the bone marrow and peripheral blood in WIBC-9, included a large number of FLT1+(98.9%/97.5%), and TIE2+(71.8%/64.2%) but include small number of VE-cadherin (23.2%/30.6%) and CD31+(20.9%/20.9%).

Bottom Line: The endothelial precursor cell population was 14% or greater in the gated lymphocyte-size fraction of the inflammatory breast cancer xenograft named WIBC-9, which exhibits a hypervascular structure and de novo formation of vascular channels, namely vasculogenic mimicry (Shirakawa et al, 2001).Furthermore, they demonstrated significantly higher levels of murine vascular endothelial growth factor and interleukin-8 in culture supernatant on time course by enzyme-linked immunosorbent assay.These findings constitute direct evidence that breast cancer induces postnatal vasculogenesis in vivo.

View Article: PubMed Central - PubMed

Affiliation: Pharmacology Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-Ku, Tokyo 104-0045, Japan. hwakasug@gan2.ncc.go.jp

Show MeSH
Related in: MedlinePlus