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Uptake and localisation of mTHPC (Foscan) and its 14C-labelled form in normal and tumour tissues of the hamster squamous cell carcinoma model: a comparative study.

Blant SA, Glanzmann TM, Ballini JP, Wagnières G, van den Bergh H, Monnier P - Br. J. Cancer (2002)

Bottom Line: The majority of mTHPC was found at around 48 h in smooth muscle and at 96 h in healthy cheek pouch mucosa and early malignant lesions.Soon after the injection, a significant selectivity was noted for advanced squamous cell carcinoma as compared to healthy cheek pouch mucosa or striated muscle.A significant difference in mTHPC localisation and quantity was also observed between striated and smooth muscle during the first 48 h following the injection.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathology, CHUV-Hospital, Bugnon 21, CH-1011 Lausanne, Switzerland. snezana.andrejevic@chuv.hospvd.ch

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Related in: MedlinePlus

Macroscopic appearance on the left side and histology on the right side of the hamster cheek pouch during various steps of squamous cell carcinogenesis. (A) Smooth and uniform surface of the healthy cheek pouch with thin mucosa; corresponding histology showing the top 3–4 layers of squamous epithelial cells (E) including well-delimited basal membrane (BM); lamina propria (LP) containing stromal cells, fibro-connective tissue and blood vessels and underlying striated muscle (SM). (B) Early dysplastic changes varying from low- to high-grade dysplasia appear between 6 and 9 weeks after DMBA application. Macroscopically, the cheek pouch mucosa is more-or-less thick, showing erythroleukoplastic changes. Histologically, this corresponds to an abnormal proliferation of epithelial cells with large nuclei, abundant cytoplasm and frequent mitotic figures. BM is less well delimited. No isolated tumour cells are visible in the lamina propria. (C) About 10 weeks after DMBA application, erythroleucoplastic changes, irregularity and thickening of the mucosa become much more severe. Histologically, besides the cytonuclear abnormalities, a small island of isolated tumour cells appears in the lamina propria. This stage corresponds to μ-invasive carcinoma often associated with slight peritumoural inflammation. (D) At 16 weeks following DMBA application, the bulky formation appears on the painted cheek pouch surface, corresponding to more-or-less well-differentiated SCC. At this stage, a tumour shows a highly infiltrative pattern and large tumour nests invade the lamina propria and striated muscle. The lesions are highly vascularised and tumour-associate inflammation is abundant. Original magnification ×10 (A,D) and ×20 (B,C).
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fig1: Macroscopic appearance on the left side and histology on the right side of the hamster cheek pouch during various steps of squamous cell carcinogenesis. (A) Smooth and uniform surface of the healthy cheek pouch with thin mucosa; corresponding histology showing the top 3–4 layers of squamous epithelial cells (E) including well-delimited basal membrane (BM); lamina propria (LP) containing stromal cells, fibro-connective tissue and blood vessels and underlying striated muscle (SM). (B) Early dysplastic changes varying from low- to high-grade dysplasia appear between 6 and 9 weeks after DMBA application. Macroscopically, the cheek pouch mucosa is more-or-less thick, showing erythroleukoplastic changes. Histologically, this corresponds to an abnormal proliferation of epithelial cells with large nuclei, abundant cytoplasm and frequent mitotic figures. BM is less well delimited. No isolated tumour cells are visible in the lamina propria. (C) About 10 weeks after DMBA application, erythroleucoplastic changes, irregularity and thickening of the mucosa become much more severe. Histologically, besides the cytonuclear abnormalities, a small island of isolated tumour cells appears in the lamina propria. This stage corresponds to μ-invasive carcinoma often associated with slight peritumoural inflammation. (D) At 16 weeks following DMBA application, the bulky formation appears on the painted cheek pouch surface, corresponding to more-or-less well-differentiated SCC. At this stage, a tumour shows a highly infiltrative pattern and large tumour nests invade the lamina propria and striated muscle. The lesions are highly vascularised and tumour-associate inflammation is abundant. Original magnification ×10 (A,D) and ×20 (B,C).

Mentions: Female Syrian hamsters (BRL, Fuellinsdorf, CH) weighing 140–160 g, housed at room temperature with 12 h light/dark cycle were used in this study. Free access to food and drinking water was allowed throughout the experiments. The animals were divided into two groups (early and advanced SCC) prior to chemically induced carcinogenesis. Early and advanced SCCs were chemically induced by topical application of 5% oily DMBA (Sigma Chemicals, Co., St. Louis, MO, USA) solution in the hamster's left cheek pouch mucosa three times weekly during 10–12 weeks (early SCC) and 14–16 weeks (advanced SCC), respectively. The contralateral buccal pouch mucosa, which was not painted with DMBA, served as the healthy mucosa control (Figure 1AFigure 1


Uptake and localisation of mTHPC (Foscan) and its 14C-labelled form in normal and tumour tissues of the hamster squamous cell carcinoma model: a comparative study.

Blant SA, Glanzmann TM, Ballini JP, Wagnières G, van den Bergh H, Monnier P - Br. J. Cancer (2002)

Macroscopic appearance on the left side and histology on the right side of the hamster cheek pouch during various steps of squamous cell carcinogenesis. (A) Smooth and uniform surface of the healthy cheek pouch with thin mucosa; corresponding histology showing the top 3–4 layers of squamous epithelial cells (E) including well-delimited basal membrane (BM); lamina propria (LP) containing stromal cells, fibro-connective tissue and blood vessels and underlying striated muscle (SM). (B) Early dysplastic changes varying from low- to high-grade dysplasia appear between 6 and 9 weeks after DMBA application. Macroscopically, the cheek pouch mucosa is more-or-less thick, showing erythroleukoplastic changes. Histologically, this corresponds to an abnormal proliferation of epithelial cells with large nuclei, abundant cytoplasm and frequent mitotic figures. BM is less well delimited. No isolated tumour cells are visible in the lamina propria. (C) About 10 weeks after DMBA application, erythroleucoplastic changes, irregularity and thickening of the mucosa become much more severe. Histologically, besides the cytonuclear abnormalities, a small island of isolated tumour cells appears in the lamina propria. This stage corresponds to μ-invasive carcinoma often associated with slight peritumoural inflammation. (D) At 16 weeks following DMBA application, the bulky formation appears on the painted cheek pouch surface, corresponding to more-or-less well-differentiated SCC. At this stage, a tumour shows a highly infiltrative pattern and large tumour nests invade the lamina propria and striated muscle. The lesions are highly vascularised and tumour-associate inflammation is abundant. Original magnification ×10 (A,D) and ×20 (B,C).
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fig1: Macroscopic appearance on the left side and histology on the right side of the hamster cheek pouch during various steps of squamous cell carcinogenesis. (A) Smooth and uniform surface of the healthy cheek pouch with thin mucosa; corresponding histology showing the top 3–4 layers of squamous epithelial cells (E) including well-delimited basal membrane (BM); lamina propria (LP) containing stromal cells, fibro-connective tissue and blood vessels and underlying striated muscle (SM). (B) Early dysplastic changes varying from low- to high-grade dysplasia appear between 6 and 9 weeks after DMBA application. Macroscopically, the cheek pouch mucosa is more-or-less thick, showing erythroleukoplastic changes. Histologically, this corresponds to an abnormal proliferation of epithelial cells with large nuclei, abundant cytoplasm and frequent mitotic figures. BM is less well delimited. No isolated tumour cells are visible in the lamina propria. (C) About 10 weeks after DMBA application, erythroleucoplastic changes, irregularity and thickening of the mucosa become much more severe. Histologically, besides the cytonuclear abnormalities, a small island of isolated tumour cells appears in the lamina propria. This stage corresponds to μ-invasive carcinoma often associated with slight peritumoural inflammation. (D) At 16 weeks following DMBA application, the bulky formation appears on the painted cheek pouch surface, corresponding to more-or-less well-differentiated SCC. At this stage, a tumour shows a highly infiltrative pattern and large tumour nests invade the lamina propria and striated muscle. The lesions are highly vascularised and tumour-associate inflammation is abundant. Original magnification ×10 (A,D) and ×20 (B,C).
Mentions: Female Syrian hamsters (BRL, Fuellinsdorf, CH) weighing 140–160 g, housed at room temperature with 12 h light/dark cycle were used in this study. Free access to food and drinking water was allowed throughout the experiments. The animals were divided into two groups (early and advanced SCC) prior to chemically induced carcinogenesis. Early and advanced SCCs were chemically induced by topical application of 5% oily DMBA (Sigma Chemicals, Co., St. Louis, MO, USA) solution in the hamster's left cheek pouch mucosa three times weekly during 10–12 weeks (early SCC) and 14–16 weeks (advanced SCC), respectively. The contralateral buccal pouch mucosa, which was not painted with DMBA, served as the healthy mucosa control (Figure 1AFigure 1

Bottom Line: The majority of mTHPC was found at around 48 h in smooth muscle and at 96 h in healthy cheek pouch mucosa and early malignant lesions.Soon after the injection, a significant selectivity was noted for advanced squamous cell carcinoma as compared to healthy cheek pouch mucosa or striated muscle.A significant difference in mTHPC localisation and quantity was also observed between striated and smooth muscle during the first 48 h following the injection.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathology, CHUV-Hospital, Bugnon 21, CH-1011 Lausanne, Switzerland. snezana.andrejevic@chuv.hospvd.ch

Show MeSH
Related in: MedlinePlus